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The map label for this gene is wcaF

Identifier: 30063505

GI number: 30063505

Start: 2116826

End: 2117374

Strand: Reverse

Name: wcaF

Synonym: S2240

Alternate gene names: 30063505

Gene position: 2117374-2116826 (Counterclockwise)

Preceding gene: 30063508

Following gene: 30063504

Centisome position: 46.04

GC content: 47.72

Gene sequence:

>549_bases
ATGCAAGATTTAAGCGGTTTCTCGGTGCCGAAAGGGTTCCGGGGCGGCAACGCTATTAAAGTGCAATTATGGTGGGCAGT
ACAGGCAACAATATTTGCCTGGTCGCCACAAGTATTGTATCGCTGGCGGGCTTTTTTATTACGTTTATTCGGAGCAAAAA
TAGGAAAAAACGTAGTTATTCGTCCGTCAGTAAAAATTACCTATCCGTGGAAATTAACCTTAGGTGATTACGCGTGGGTT
GGCGATGACGTCAATTTATATACCCTCGGTGAAATAACCATTGGCGCACATTCGGTGATATCGCAAAAAAGTTATTTATG
CACCGGTAGCCACGACCATGCAAGTCAACATTTCACCATTAACGCCACGCCTATTGTGATTGGCGAGAAATGCTGGCTGG
CAACCGATGTCTTTGTTGCCCCAGGCGTCACAATCGGCGACGGCACCGTCGTGGGTGCACGAAGCAGTGTTTTTAAATCG
CTTCCGGCAAATGTGGTTTGCCGGGGGAATCCCGCAGTGGTGATACGCGAACGCGTTGAAACTGAATAA

Upstream 100 bases:

>100_bases
TGTACCTGGCTTCTGGGCAGAATTATCCTGGCATTTACGCCAACGTACGACAGGTAAGACTAAAGCCTTATATAACAAAG
TCTGAATATAAGGAAAACCA

Downstream 100 bases:

>100_bases
ATTCAAAAATACAGAGGAATAAGACATGTCAAAAGTCGCTCTCATCACCGGTGTAACCGGACAAGACGGTTCTTACCTGG
CAGAGTTTCTGCTGGAAAAA

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRERVETE

Sequences:

>Translated_182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRERVETE
>Mature_182_residues
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWV
GDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
LPANVVCRGNPAVVIRERVETE

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1788367, Length=182, Percent_Identity=100, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1786537, Length=138, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788345, Length=118, Percent_Identity=33.8983050847458, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1786664, Length=121, Percent_Identity=29.7520661157025, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=144, Percent_Identity=27.0833333333333, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WCAF_ECOLI (P0ACD2)

Other databases:

- EMBL:   U38473
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   E64971
- RefSeq:   AP_002654.1
- RefSeq:   NP_416558.1
- ProteinModelPortal:   P0ACD2
- SMR:   P0ACD2
- STRING:   P0ACD2
- EnsemblBacteria:   EBESCT00000001102
- EnsemblBacteria:   EBESCT00000016732
- GeneID:   946578
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2039
- KEGG:   eco:b2054
- EchoBASE:   EB3344
- EcoGene:   EG13574
- eggNOG:   COG0110
- GeneTree:   EBGT00050000009072
- HOGENOM:   HBG510560
- OMA:   AWVGDEV
- ProtClustDB:   PRK10502
- BioCyc:   EcoCyc:G7099-MONOMER
- Genevestigator:   P0ACD2
- InterPro:   IPR018357
- InterPro:   IPR011004

Pfam domain/function: SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 19962; Mature: 19962

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00101 HEXAPEP_TRANSFERASES; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTI
CCCEEEEEEEEEEECCEEEECCCCEEEEEEEEEECCHHHHCCCCEEEECCCCCCCCEEEE
NATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVE
ECCEEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCEEEEEEECC
TE
CC
>Mature Secondary Structure
MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRAFLLRLFGAKIGKNVVI
CCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHCCCEEE
RPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQKSYLCTGSHDHASQHFTI
CCCEEEEEEEEEEECCEEEECCCCEEEEEEEEEECCHHHHCCCCEEEECCCCCCCCEEEE
NATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVE
ECCEEEEECCEEEEEEEEECCCEEECCCEEECCHHHHHHHCCCCEEECCCCEEEEEEECC
TE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503