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The map label for this gene is rfbB

Identifier: 30063492

GI number: 30063492

Start: 2100470

End: 2101555

Strand: Reverse

Name: rfbB

Synonym: S2227

Alternate gene names: 30063492

Gene position: 2101555-2100470 (Counterclockwise)

Preceding gene: 30063493

Following gene: 30063491

Centisome position: 45.69

GC content: 44.2

Gene sequence:

>1086_bases
GTGAAGATACTTGTTACTGGTGGCGCAGGGTTTATTGGTTCTGCTGTTGTTCGTCACATAATAAATAATACGCAAGATAG
TGTTGTTAATGTCGATAAATTAACATACGCCGGGAACCTGGAATCACTCGCTGATGTTTCTGATTCTGAACGTTATGCTT
TTGAGCATGCAGATATCTGCGATGCTGTAGCCATGTCGCGTATTTTCGCACAGCACCAGCCAGACGCGGTGATGCACCTG
GCGGCAGAGAGCCACGTTGACCGTTCAATAACGGGCCCTGCGGCATTTATTGAAACCAATATTGTGGGGACTTATGTTCT
TTTAGAAGCGGCGCGCAATTACTGGTCTGCTCTGAATGATGAAAAGAAAAAAAGTTTCCGCTTTCATCATATTTCTACTG
ATGAAGTTTATGGTGACTTACCCCATCCGGATGAAGCAAATAATAACGAAGCGTTACCGCTATTTACGGAAACGACAGCT
TACGCGCCAAGTAGCCCGTATTCTGCGTCTAAAGCTTCCAGCGATCATTTGGTACGTGCCTGGAAGCGGACTTATGGTTT
ACCAACCATTGTGACTAATTGCTCGAACAATTATGGTCCTTATCACTTCCCGGAAAAACTTATTCCATTGGTTATTCTGA
ATGCTCTGGAAGGTAAGGCATTACCTATTTATGGTAAAGGGGATCAAATTCGCGACTGGTTGTATGTAGAGGATCATGCT
CGTGCGTTATATACCGTCGTAACCGAAGGTAAAGCGGGTGAAACTTATAACATTGGTGGACATAACGAAAAGAAAAACAT
CGACGTAGTGCTCACTATTTGTGATTTGTTGGATGAGATTGTACCGAAAGAGAAATCTTATCGTGAGCAAATTACTTATG
TTGCCGATCGCCCGGGGCATGACCGCCGTTACGCCATTGATGCCGATAAAATTAGCCGCGAATTGGGCTGGAAACCGCAA
GAAACGTTTGAGAGCGGGATTCGTAAAACGGTGGAATGGTATCTGGCTAATACAAATTGGGTTGAGAATGTGAAAAGCGG
TACTTATCAGTCGTGGATTGAACAAAACTATGAGGGCCGTCAGTAA

Upstream 100 bases:

>100_bases
GCTGTTAAGCCAGGGGCGGTAGCGTGTATTAATACCTCTATTAATCAAATCTGGAGCAGTCTATTTCACAGCATGCTCTA
TAGCTATATGGAATAAAAAA

Downstream 100 bases:

>100_bases
TGAATATCCTGCTTTTCGGCAAAACAGGGCAGGTGGGTTGGGAACTGCAGCGTGCTCTGGCTCCTCTGGGTAACTTGATT
GCGCTCGATGTTCACTCCAC

Product: dTDP-glucose 4,6 dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQ
ETFESGIRKTVEWYLANTNWVENVKSGTYQSWIEQNYEGRQ

Sequences:

>Translated_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQ
ETFESGIRKTVEWYLANTNWVENVKSGTYQSWIEQNYEGRQ
>Mature_361_residues
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHL
AAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQ
ETFESGIRKTVEWYLANTNWVENVKSGTYQSWIEQNYEGRQ

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=35.1906158357771, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=23.5119047619048, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI56237023, Length=347, Percent_Identity=28.2420749279539, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI56118217, Length=347, Percent_Identity=28.2420749279539, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI189083684, Length=347, Percent_Identity=28.2420749279539, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=95.0138504155125, Blast_Score=717, Evalue=0.0,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=74.2209631728045, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1786974, Length=356, Percent_Identity=25, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788366, Length=373, Percent_Identity=24.9329758713137, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788365, Length=353, Percent_Identity=22.6628895184136, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=335, Percent_Identity=35.2238805970149, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=337, Percent_Identity=34.1246290801187, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI71982035, Length=373, Percent_Identity=26.8096514745308, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=375, Percent_Identity=26.6666666666667, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=23.2352941176471, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=374, Percent_Identity=26.4705882352941, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19923002, Length=373, Percent_Identity=25.7372654155496, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=23.6686390532544, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24158427, Length=290, Percent_Identity=25.5172413793103, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40576; Mature: 40576

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYAFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCHHHHHHHHHH
DAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALND
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
EKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTAYAPSSPYSASKASSDHLVRA
HHHHCEEEEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHEEECCCCC
DRRYAIDADKISRELGWKPQETFESGIRKTVEWYLANTNWVENVKSGTYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCCC
Q
C
>Mature Secondary Structure
MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLADVSDSERYAFEHADIC
CEEEEECCCCHHHHHHHHHHHCCCCHHEEEHHHEEECCCHHHHHCCCCCCHHHHHHHHHH
DAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGTYVLLEAARNYWSALND
HHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCH
EKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTAYAPSSPYSASKASSDHLVRA
HHHHCEEEEECCHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHH
WKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALPIYGKGDQIRDWLYVEDHA
HHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEHHC
RALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIVPKEKSYREQITYVADRPGH
CEEEEEEECCCCCCEEECCCCCCCCCCHHEEHHHHHHHHHCCCCHHHHHHHHEEECCCCC
DRRYAIDADKISRELGWKPQETFESGIRKTVEWYLANTNWVENVKSGTYQSWIEQNYEGR
CCEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHCCCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503 [H]