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The map label for this gene is ydhE

Identifier: 30063179

GI number: 30063179

Start: 1762736

End: 1764109

Strand: Direct

Name: ydhE

Synonym: S1823

Alternate gene names: 30063179

Gene position: 1762736-1764109 (Clockwise)

Preceding gene: 30063177

Following gene: 30063181

Centisome position: 38.33

GC content: 52.98

Gene sequence:

>1374_bases
GTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGC
GATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCT
GGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGCATTAACGCCGGTTATCGCGCAATTAAATGGTTCCGGTCGA
CGTGAGCGCATTGCGCATCAGGTGCGACAAGGTTTCTGGCTGGCAGGTTTTGTTTCCGTTCTCATTATGCTGGTGCTGTG
GAATGCAGGTTACATTATCCGCTACATGGAAAACATCGATCCGGCTCTGGCGGACAAAGCCGTGGGTTATCTGCGTGCGT
TGTTGTGGGGCGCGCCGGGATATCTGTTCTTCCAGGTTGCCCGTAACCAATGTGAAGGTCTGGCAAAAACCAAGCCGGGT
ATGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGA
GCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAAC
GCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCTAAGCCCGAGCCTGCGGTTATGAAACGACTGATTCAA
CTCGGTTTGCCGATTGCGCTGGCACTGTTCTTTGAAGTGACACTGTTTGCCGTCGTAGCTCTGTTAGTGTCTCCGCTCGG
TATTGTTGATGTCGCAGGACACCAGATTGCCCTGAACTTTAGTTCACTGATGTTTGTGCTTCCAATGTCGCTGGCGGCAG
CGGTAACTATCCGCGTAGGTTATCGTCTGGGTCAGGGCTCAACGCTGGATGCGCAAACCGCTGCGCGGACCGGGCTTATG
GTGGGTGTCTGTATGGCAACCCTGACGGCCATTTTCACGGTTTCACTGCGGGAGCAAATCGCCCTGTTGTACAACGACAA
TCCCGAGGTTGTAACGCTGGCTGCGCATTTGATGTTGCTGGCGGCGGTATATCAGATTTCTGACTCAATCCAGGTGATTG
GCAGTGGGATTTTGCGTGGTTATAAAGATACGCGTTCCATTTTCTATATCACCTTTACAGCTTACTGGGTGCTGGGCTTG
CCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTAT
CGGCCTGACATCGGCAGCCATTATGATGATGCTGCGTATGCGGTTCCTGCAACGTATGCCGTCAGCCATCATTCTGCAAC
GAGCGTCCCGCTAA

Upstream 100 bases:

>100_bases
TCCTCGCTACAATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAAC
TACAAATAAAAGGTGTTCAC

Downstream 100 bases:

>100_bases
TAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTGTTTTTACGCAGGCTGGAGCGTTGCGCCAATCCCGTCTTCGTCT
GGCTGTAATTTCAGAGCGTT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPEPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAIILQRASR

Sequences:

>Translated_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPEPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAIILQRASR
>Mature_457_residues
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGR
RERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPEPAVMKRLIQ
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLM
VGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGL
PSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAIILQRASR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=442, Percent_Identity=21.4932126696833, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI153792564, Length=424, Percent_Identity=20.0471698113208, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI38261960, Length=460, Percent_Identity=19.7826086956522, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=99.3435448577681, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=459, Percent_Identity=23.7472766884532, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321821, Length=385, Percent_Identity=20.7792207792208, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320545, Length=435, Percent_Identity=20.6896551724138, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49556; Mature: 49556

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRYMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPEPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRFLQRMPSAIILQRASR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC
>Mature Secondary Structure
MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDMAAVAIGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLIMLVLWNAGYIIRYMENID
HCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHCC
PALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPGMVMGFIGLLVNIPVNYIFIY
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHCCEEEEEEE
GHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSMRDIRNEKGTAKPEPAVMKRLIQ
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQIALNFSSLMFVLPMSLAAAVTIRVG
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHC
YRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTVSLREQIALLYNDNPEVVTLAAHLMLL
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
AAVYQISDSIQVIGSGILRGYKDTRSIFYITFTAYWVLGLPSGYILALTDLVVEPMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCHH
FWIGFIIGLTSAAIMMMLRMRFLQRMPSAIILQRASR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA