Click here to switch to the map view.

The map label for this gene is infC

Identifier: 30063015

GI number: 30063015

Start: 1584854

End: 1585288

Strand: Direct

Name: infC

Synonym: S1631

Alternate gene names: 30063015

Gene position: 1584854-1585288 (Clockwise)

Preceding gene: 30063014

Following gene: 30063016

Centisome position: 34.46

GC content: 47.36

Gene sequence:

>435_bases
GTGAGTCTGAGAGAAGCTCTGGAGAAAGCAGAAGAAGCCGGAGTAGACTTAGTCGAGATCAGCCCTAACGCCGAGCCGCC
GGTTTGTCGTATAATGGATTACGGCAAATTCCTCTATGAAAAGAGCAAGTCTTCTAAGGAACAGAAGAAAAAGCAAAAAG
TTATCCAGGTTAAGGAAATTAAATTCCGTCCTAGTACAGATGAAGGCGACTATCAGGTAAAACTCCGCAGCCTGATTCGC
TTTCTCGAAGAGGGTGATAAAGCCAAAATCACGCTGCGTTTCCGCGGTCGTGAGATGGCGCACCAGCAAATCGGTATGGA
AGTGCTTAATCGCGTGAAAGACGATTTGCAAGAACTGGCAGTAGTCGAATCCTTCCCAACGAAGATCGAAGGCCGCCAGA
TGATCATGGTGCTCGCTCCTAAGAAGAAACAGTAA

Upstream 100 bases:

>100_bases
CGGAAAACGAGTTCAAACGGCGCGCCCTAACCGTATCAATGGCGAAATTCGCGCCCAGGAAGTTCGCTTAACAGGTCTGG
AAGGCGAGCAGCTTGGTATT

Downstream 100 bases:

>100_bases
GGCCTTCAAGTAGCACTATCTGTGGAGCCTTTGGGCTTCACAGGCATTGTTCGCCTTTGTTTCGTTTATTAACAATGCGA
AGTGGAAGTTATTAAAATGC

Product: translation initiation factor IF-3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 144; Mature: 143

Protein sequence:

>144_residues
MSLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPSTDEGDYQVKLRSLIR
FLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ

Sequences:

>Translated_144_residues
MSLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPSTDEGDYQVKLRSLIR
FLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ
>Mature_143_residues
SLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPSTDEGDYQVKLRSLIRF
LEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVESFPTKIEGRQMIMVLAPKKKQ

Specific function: IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins

COG id: COG0290

COG function: function code J; Translation initiation factor 3 (IF-3)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-3 family

Homologues:

Organism=Escherichia coli, GI1788012, Length=144, Percent_Identity=98.6111111111111, Blast_Score=286, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IF3_SHIFL (Q83L38)

Other databases:

- EMBL:   AE005674
- EMBL:   AE014073
- RefSeq:   NP_707396.1
- RefSeq:   NP_837186.1
- ProteinModelPortal:   Q83L38
- EnsemblBacteria:   EBESCT00000086193
- EnsemblBacteria:   EBESCT00000092920
- GeneID:   1026392
- GeneID:   1077906
- GenomeReviews:   AE005674_GR
- GenomeReviews:   AE014073_GR
- KEGG:   sfl:SF1513
- KEGG:   sfx:S1631
- GeneTree:   EBGT00050000012022
- HOGENOM:   HBG744268
- ProtClustDB:   PRK00028
- BioCyc:   SFLE198214:AAN43103.1-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00080
- InterPro:   IPR019813
- InterPro:   IPR001288
- InterPro:   IPR019815
- InterPro:   IPR019814
- Gene3D:   G3DSA:3.10.20.80
- Gene3D:   G3DSA:3.30.110.10
- PANTHER:   PTHR10938
- TIGRFAMs:   TIGR00168

Pfam domain/function: PF00707 IF3_C; PF05198 IF3_N; SSF54364 IF3; SSF55200 IF3

EC number: NA

Molecular weight: Translated: 16668; Mature: 16537

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00938 IF3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEI
CCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHE
KFRPSTDEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELA
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VVESFPTKIEGRQMIMVLAPKKKQ
HHHHCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SLREALEKAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEI
CHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHE
KFRPSTDEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELA
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
VVESFPTKIEGRQMIMVLAPKKKQ
HHHHCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152