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The map label for this gene is puuD

Identifier: 30062818

GI number: 30062818

Start: 1355245

End: 1356021

Strand: Direct

Name: puuD

Synonym: S1385

Alternate gene names: 30062818

Gene position: 1355245-1356021 (Clockwise)

Preceding gene: 30062804

Following gene: 30062819

Centisome position: 29.47

GC content: 52.51

Gene sequence:

>777_bases
GTGAGATTGAGCATGGAAAATATAATGAACAATCCGGTTATCGGTGTCGTAATGTGCAGGAACAGGCTTAAGGGTCATGC
GACCCAGACTCTGCAAGAAAAGTACCTGAATGCCATCATCCACGCAGGCGGCTTGCCAATTGCGCTACCACATGCGCTGG
CGGAACCGTCGTTACTTGAACAACTTTTGCCGAAACTCGATGGCATTTATCTTCCTAGTAGTCCCAGCAATGTGCAGCCG
CACCTATATGGTGAAAACGGCGATGAGCCTGACGCCGATCCCGGGCGTGATCTTCTGAGCATGGCGATAATTAATGCCGC
ACTCGAAAGGCGCATCCCCATTTTCGCCATCTGCCGGGGTTTACAAGAACTGGTGGTGGCAACCGGTGGTTCGTTGCATC
GCAAGCTGTGCGAACAGCCTGAATTGCTGGAACATCGGGAAGATCCCGAACTGCCGGTGGAACAGCAATATGCACCGTCG
CATGAAGTTCAGGTTGAAGAGGGGGGATTACTGTCTGCGTTGTTACCTGAATGTAGCAACTTTTGGGTAAACTCTCTACA
TGGACAAGGGGCGAAGGTCGTTAGCCCACGGTTGCGTGTTGAAGCTCGCTCGCCGGATGGCTTAGTTGAGGCGGTTAGCG
TCATCAATCATCCTTTTGCGCTGGGCGTACAGTGGCACCCGGAATGGAACAGTAGCGAGTACGCGCTTTCGCGTATATTG
TTCGAGGGCTTTATCACCGCTTGGCAGCACCATATCGCTGAAAAACAGCGACTCTGA

Upstream 100 bases:

>100_bases
AAATTGAGTTTGCAAAAATGAAAATCCACTGCTAGATTGAAAAAATATTGAACATAAAGGTCATTTAAAGCGCAGTAATG
GCGATAATTTAGTCCACTTT

Downstream 100 bases:

>100_bases
CCACTACAGTTTAAGGAAATGCAAATATGAGTGATGAGGGACTGGCGCCAGGAAAACGCTTGTCGGAAATCCGCCAGCAG
CAGGGGCTTTCACAACGTCG

Product: gamma-glutamyl-gamma-aminobutyrate hydrolase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRLSMENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPSSPSNVQP
HLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPS
HEVQVEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRIL
FEGFITAWQHHIAEKQRL

Sequences:

>Translated_258_residues
MRLSMENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPSSPSNVQP
HLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPS
HEVQVEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRIL
FEGFITAWQHHIAEKQRL
>Mature_258_residues
MRLSMENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPSSPSNVQP
HLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPS
HEVQVEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRIL
FEGFITAWQHHIAEKQRL

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=254, Percent_Identity=99.2125984251969, Blast_Score=510, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28614; Mature: 28614

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSMENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLE
CCCCHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHH
QLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRG
HHHHHHCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSN
HHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRIL
HHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHH
FEGFITAWQHHIAEKQRL
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRLSMENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLE
CCCCHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHH
QLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRG
HHHHHHCCEECCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSN
HHHHHHHCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
FWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRIL
HHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHCCCEEECEEECCCCCCHHHHHHHHH
FEGFITAWQHHIAEKQRL
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]