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The map label for this gene is flgM

Identifier: 30062609

GI number: 30062609

Start: 1120488

End: 1120781

Strand: Reverse

Name: flgM

Synonym: S1155

Alternate gene names: 30062609

Gene position: 1120781-1120488 (Counterclockwise)

Preceding gene: 30062610

Following gene: 30062608

Centisome position: 24.37

GC content: 53.06

Gene sequence:

>294_bases
ATGAGTATTGATCGCACTTCGCCTCTGAAGCCTGTAAGCACCGTTCAACCGCGCGAAACCACCGACGCGCCGGTAACGAA
CAGCCGGGCGGCAAAAACAACCGCCTCCACCAGCACCAGTGTGACGTTAAGCGACGCGCAAGCAAAACTGATGCAACCCG
GCAGCAGTGATATCAATCTTGAACGTGTCGAAGCGTTAAAACTGGCGATTCGTAACGGTGAACTAAAAATGGACACCGGC
AAAATTGCCGATGCGCTGATCAACGAAGCGCAGCAAGACTTGCAGAGTAACTGA

Upstream 100 bases:

>100_bases
TGGGAATATTCTTATAAACCTGTAAGCTGTTAAAGATTACCCGTCCCTTGCCGATAAATAAGCAACACATGATAAAAGCG
CCCTCAATGAGGAATAAACC

Downstream 100 bases:

>100_bases
TCGTATGACACGTCTTGCAGAGATCCTCGACCAGATGTCCGCTGTGCTTAACGATCTCAAAACGGTAATGGATCAAGAGC
AGCAACATCTCTCTATGGGG

Product: anti-sigma28 factor FlgM

Products: NA

Alternate protein names: Anti-sigma-28 factor

Number of amino acids: Translated: 97; Mature: 96

Protein sequence:

>97_residues
MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTG
KIADALINEAQQDLQSN

Sequences:

>Translated_97_residues
MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTG
KIADALINEAQQDLQSN
>Mature_96_residues
SIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGK
IADALINEAQQDLQSN

Specific function: Responsible for the coupling of flagellin expression to flagellar assembly by preventing expression of the flagellin genes when a component of the middle class of proteins is defective. It negatively regulates flagellar genes by inhibiting the activity of

COG id: COG2747

COG function: function code KNU; Negative regulator of flagellin synthesis (anti-sigma28 factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flgM family

Homologues:

Organism=Escherichia coli, GI1787311, Length=97, Percent_Identity=100, Blast_Score=192, Evalue=4e-51,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): FLGM_ECOLI (P0AEM4)

Other databases:

- EMBL:   U19773
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64850
- RefSeq:   AP_001697.1
- RefSeq:   NP_415589.1
- ProteinModelPortal:   P0AEM4
- IntAct:   P0AEM4
- STRING:   P0AEM4
- EnsemblBacteria:   EBESCT00000000745
- EnsemblBacteria:   EBESCT00000000746
- EnsemblBacteria:   EBESCT00000017251
- GeneID:   946684
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1058
- KEGG:   eco:b1071
- EchoBASE:   EB2530
- EcoGene:   EG12664
- eggNOG:   COG2747
- GeneTree:   EBGT00050000010052
- HOGENOM:   HBG648451
- OMA:   MSIDRTQ
- ProtClustDB:   PRK10810
- BioCyc:   EcoCyc:G369-MONOMER
- Genevestigator:   P0AEM4
- GO:   GO:0019861
- GO:   GO:0006350
- InterPro:   IPR007412
- TIGRFAMs:   TIGR03824

Pfam domain/function: PF04316 FlgM

EC number: NA

Molecular weight: Translated: 10341; Mature: 10209

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLSDAQAKLMQPGSSDINL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCH
ERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLSDAQAKLMQPGSSDINL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCH
ERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN
HHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 9095196