Click here to switch to the map view.

The map label for this gene is cmr

Identifier: 30062324

GI number: 30062324

Start: 825252

End: 826478

Strand: Direct

Name: cmr

Synonym: S0838

Alternate gene names: 30062324

Gene position: 825252-826478 (Clockwise)

Preceding gene: 30062321

Following gene: 30062328

Centisome position: 17.94

GC content: 52.16

Gene sequence:

>1227_bases
ATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATT
TTCAACCTATATCGGCAACGATATGATCCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGG
TTCCTACTTCGATGACCGCGTATCTGGCGGGCGGGATGTTTTTACAATGGCTCCTGGGGCCGCTGTCGGATCGTATTGGT
CGCCGTCCGGTGATGCTGGCGGGTGTGGTGTGGTTTATCGTCACCTGTCTGGCAATATTACTGGCGCAAAACATTGAACA
ATTCACCCTGTTGCGCTTCTTGCAGGGCATAAGCTTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCT
TCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTG
GGCGCGGCGTGGATCCATGTGCTGCCTTGGGAGGGGATGTTTGTTTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGG
TCTGCAACGAGCTATGCCTGAAACCGCCATGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGC
TGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGGATTCGTTAGCCTGCCATTGCTGGCGTGGATCGCCCAGTCG
CCGATTATCATCATTACCGGGGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGCGTTAAT
TGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGCCGATTA
TGATTGGTCTGTTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACCGCCGGGTTAAGTATT
TATGCTTTCGGTATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGGTACGGT
TTCTGCGGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTCGGTATTGAAATCAGCAAACATGCCTGGCTGAACGGGG
GCAACGGACTGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAAGATAAA
CAGATGGGAAATTCTCACGAAGGGTAA

Upstream 100 bases:

>100_bases
GTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGCGCTTTTATTAAACTCTGCGCGATT
ATTATTGGCGAAGAAATTGC

Downstream 100 bases:

>100_bases
AAAAATGCCTGATTGATTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATC
CCTTCACCTACGCTGGCAAG

Product: chloramphenicol resistance pump Cmr

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKNGRFVAGALGFVSLPLLAWIAQS
PIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSI
YAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDK
QMGNSHEG

Sequences:

>Translated_408_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKNGRFVAGALGFVSLPLLAWIAQS
PIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSI
YAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDK
QMGNSHEG
>Mature_408_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKNGRFVAGALGFVSLPLLAWIAQS
PIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSI
YAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDK
QMGNSHEG

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=410, Percent_Identity=99.0243902439024, Blast_Score=801, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=376, Percent_Identity=41.4893617021277, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI48994889, Length=217, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790146, Length=145, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788509, Length=304, Percent_Identity=24.3421052631579, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44202; Mature: 44202

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
YLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISFC
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKNGRFVAGALGFVSLPLLAWIAQS
HHHHHHHHHHHCHHHHHHHCCCCCHHHHCCCCEEHEECCCEEEHHHHHHHHHHHHHHHCC
PIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVA
CEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFT
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHHHH
VGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
YLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLAQNIEQFTLLRFLQGISFC
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKNGRFVAGALGFVSLPLLAWIAQS
HHHHHHHHHHHCHHHHHHHCCCCCHHHHCCCCEEHEECCCEEEHHHHHHHHHHHHHHHCC
PIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVA
CEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFT
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHHHH
VGIEISKHAWLNGGNGLFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]