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The map label for this gene is ybiK

Identifier: 30062311

GI number: 30062311

Start: 806889

End: 807854

Strand: Direct

Name: ybiK

Synonym: S0821

Alternate gene names: 30062311

Gene position: 806889-807854 (Clockwise)

Preceding gene: 161486526

Following gene: 30062312

Centisome position: 17.54

GC content: 56.42

Gene sequence:

>966_bases
ATGGGCAAAGCAGTCATTGCAATTCATGGTGGCGCAGGTGCAATTAGCCGCGCGCAGATGAGTCTGCAACAGGAATTACG
CTACATCGAGGCGTTGTCTGCCATTGTTGAAACCGGGCAGAAAATGCTGGAAGCCGGCGAAAGTGCGCTGGATGTGGTGA
CGGAAGCGGTGCGTCTGCTGGAAGAGTGTCCACTGTTTAACGCCGGAATTGGCGCTGTCTTTACGCGTGATGAAACCCAT
GAACTGGACGCCTGTGTGATGGATGGTAACACCCTGAAAGCCGGTGCGGTGGCGGGCGTTAGTCATCTGCGTAATCCGGT
TCTTGCCGCCCGGCTGGTGATGGAGCAAAGCCCGCATGTGATGATGATTGGCGAAGGGGCAGAAAATTTTGCGTTTGCTC
ATGGCATGGAGCGCGTCTCACCGGAGATTTTCTCCACGCCTTTGCGTTATGAACAACTACTGGCAGCGCGCGAGGAAGGG
GCAACAGTCCTCGACCATAGCGGTGCGCCACTGGATGAAAAACAGAAAATGGGCACCGTGGGGGCCGTGGCGTTGGATTT
AGACGGCAATCTGGCGGCAGCCACGTCCACAGGCGGAATGACCAATAAATTACCCGGACGAGTTGGCGATAGTCCCTTAG
TGGGTGCCGGATGCTACGCCAATAACGCCAGTGTGGCGGTTTCTTGTACCGGCACAGGCGAAGTCTTCATCCGCGCGCTG
GCGGCATATGACATCGCCGCGTTAATGGATTACGGCGGATTAAGTCTCGCGGAAGCCTGCGAGCGGGTAGTAATGGAAAA
ACTCCCTGCGCTTGGCGGTAGCGGTGGCTTAATCGCTATCGACCATGAAGGGAATGTCGCGCTACCGTTTAACACCGAAG
GAATGTATCGCGCCTGGGGCTACGCAGGCGATACGCCAACCACCGGTATCTACCGTGAAAAAGGGGACACCGTTGCCACA
CAGTGA

Upstream 100 bases:

>100_bases
GCCATATCCATCTTTCTATATTCAAAAATTGAATGAGTAATTCATAAAAATTCTGATATTTATAGCAAAAGTGGCGAGCC
ACCCTTAATGGACGAATACT

Downstream 100 bases:

>100_bases
TGAACTTGATGCCGGTAATGTGCTGGCGGTTGAAAATCTTAATATTGCCTTTATGCAGGACCAGCAGAAAATAGCTGCGG
TCCGCAATCTCTCTTTTAGT

Product: L-asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETH
ELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVAT
Q

Sequences:

>Translated_321_residues
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETH
ELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVAT
Q
>Mature_320_residues
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHE
LDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGA
TVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRALA
AYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=301, Percent_Identity=37.5415282392027, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI145275200, Length=301, Percent_Identity=37.5415282392027, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI98991769, Length=269, Percent_Identity=33.0855018587361, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI285002251, Length=256, Percent_Identity=28.90625, Blast_Score=88, Evalue=9e-18,
Organism=Escherichia coli, GI1787050, Length=321, Percent_Identity=99.0654205607477, Blast_Score=643, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569239, Length=252, Percent_Identity=27.7777777777778, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32564649, Length=311, Percent_Identity=26.3665594855305, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI18921183, Length=322, Percent_Identity=36.3354037267081, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21357893, Length=268, Percent_Identity=30.9701492537313, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI221330102, Length=236, Percent_Identity=33.4745762711864, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24652135, Length=236, Percent_Identity=33.4745762711864, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33386; Mature: 33255

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLL
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGATVLDHSGAPLDEKQKMGTV
EEECCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYREKGDTVATQ
CCCCCCCCCEEECCCCCCCCC
>Mature Secondary Structure 
GKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
EECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNPVLAARLVMEQSPHV
HHCCCCCCCCCEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCE
MMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEGATVLDHSGAPLDEKQKMGTV
EEECCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCE
GAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRAL
EEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCHHHHHHHH
AAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAIDHEGNVALPFNTEGMYRAWG
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCCCEEECC
YAGDTPTTGIYREKGDTVATQ
CCCCCCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]