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The map label for this gene is insF1 [C]

Identifier: 30062217

GI number: 30062217

Start: 710430

End: 711116

Strand: Reverse

Name: insF1 [C]

Synonym: S0715

Alternate gene names: 30062217

Gene position: 711116-710430 (Counterclockwise)

Preceding gene: 30062218

Following gene: 30062209

Centisome position: 15.46

GC content: 52.11

Gene sequence:

>687_bases
GTGGCACATATCCGCACTCGCGAAACATATGGAACCCGGCGGCTCCAGACGGAGCTGGCAGAGAATGGCATCATCGTTGG
TCGTGACCGACTGGCACGTCTTCGTAAGGAGCTGAGGCTACGCTGTAAGCAGAAACGCAAGTTCAGAGCGACTACGAACC
CGAACCACAATCTGCCAGTTGCGCCAAATCTGCTGAACCAGACGTTCGCTCCTACAGCACCAAATCAGGTCTGGGTGGCG
GACCTGACGTATGTTGCTGGCATCAAAGATGTTTATACGTGCGAAATTGTCGGCTACGCCATGGGAGAGCGCATGACAAA
AGAGCTGACAGGTAAAGCCCTGTTTATGGCGCTCAGGAGCCAGCGCCCACCTGCCGGGCTAATCCACCACTCTGATCGAG
GTTCACAGTACTGCGCATACGATTACCGGGTCATACAGGAGCAGTCTGGTCTGAAAACATCAATGTCGCGTAAAGGTAAC
TGTTACGACAACGCTCCGATGGAAAGCTTCTGGGGAACGCTGAAAAATGAGAGCCTGAGCCACTATCGTTTTAATAACCG
GGATGAAGCCATCTCAGTAATACGGGAATACATTGAGATTTTCTACAATCGTCAGCGTCGTCACTCTCGTCTGGGGAATA
TCTCCCCGGCAGCCTTCAGGGAAAAATATCATCAGATGGCTGCTTAA

Upstream 100 bases:

>100_bases
TCAGGTATTTGGTGTATCCAGGAGCGGTTATTACAACTGGGTACAGCATGAACCCTCAGACAGAAAACAAAGTGATGAGC
GGCTAAAACTGGAGATTAAG

Downstream 100 bases:

>100_bases
AAAAAGAACAAATGGTAGTGTCCGCTATTGCCAGTACACCTCACTGATCCAACCACTGCAAAAACCAGTAGCCGGCACTG
CCGGCGGAGGTGCCGTAGGC

Product: IS600 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGN
CYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Sequences:

>Translated_228_residues
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
DLTYVAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGN
CYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
>Mature_227_residues
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVAD
LTYVAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNC
YDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=194, Percent_Identity=31.9587628865979, Blast_Score=104, Evalue=6e-23,
Organism=Escherichia coli, GI1788405, Length=230, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787263, Length=230, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786753, Length=230, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786570, Length=230, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786490, Length=230, Percent_Identity=39.1304347826087, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789981, Length=227, Percent_Identity=33.4801762114537, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 26418; Mature: 26286

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCCHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRS
CCHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLS
CCCCCCCEEECCCCCCEEHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
HYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPV
CCCCCCHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCC
APNLLNQTFAPTAPNQVWVADLTYVAGIKDVYTCEIVGYAMGERMTKELTGKALFMALRS
CCHHHHHCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGTLKNESLS
CCCCCCCEEECCCCCCEEHHHHHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCC
HYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]