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The map label for this gene is insF1 [C]

Identifier: 30062214

GI number: 30062214

Start: 708293

End: 708667

Strand: Direct

Name: insF1 [C]

Synonym: S0708

Alternate gene names: 30062214

Gene position: 708293-708667 (Clockwise)

Preceding gene: 30062213

Following gene: 30062215

Centisome position: 15.4

GC content: 51.47

Gene sequence:

>375_bases
ATGAAAGCGCTGGAAATGGCATGGGAAACCCGTGGTAAGCCCGGCGGGGTGATGTTCCACAGCGATCAGGGCAGTCATTA
TACGAGCAGGCAGTTCCGGCAGTTATTGTGGCGATACCAGATCAGACAGAGTATGAGCCGGCGCGGAAACTGCTGGGATA
ACAGCCCAATGGAACGCTTCTTCAGGAGTCTGAAGAACGAATGGATGCCGGTGGTGGGTTACGTAAGCTTCAGCGAGGCA
GCTCACGCCATAACGGACTATATCGTTGGATATTACAGTGCACTAAGACCGCACGAATATAACGGTGGGTTACCCCCAAA
CGAATCGGAAAATCGATACTGGAAAAACTCTAACTCGGTGGCCAGTTTTTGTTGA

Upstream 100 bases:

>100_bases
GGACGGGTAAGCGCTGGGCGTACCTCCCGTTGTTCTCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCT
CGCCGGACAGCAGGCTCACC

Downstream 100 bases:

>100_bases
CCACTTCAGTGGCGGTAAGACGGGGAATATGTTTAACCTGATGTTGACCTCTAAAAAGCTGACAAAAACGGCAATTAACG
AGGCGCTCCGGAGGATGAAA

Product: IS911 orfB

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEA
AHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC

Sequences:

>Translated_124_residues
MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEA
AHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC
>Mature_124_residues
MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEA
AHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVASFC

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1788405, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1787263, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1786753, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1786570, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1786490, Length=110, Percent_Identity=37.2727272727273, Blast_Score=77, Evalue=4e-16,
Organism=Escherichia coli, GI1789981, Length=111, Percent_Identity=36.9369369369369, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 14571; Mature: 14571

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERF
CCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC
ASFC
CCCC
>Mature Secondary Structure
MKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERF
CCHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
FRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSV
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCC
ASFC
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]