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The map label for this gene is ybeM

Identifier: 30062185

GI number: 30062185

Start: 687935

End: 688498

Strand: Reverse

Name: ybeM

Synonym: S0677

Alternate gene names: 30062185

Gene position: 688498-687935 (Counterclockwise)

Preceding gene: 30062187

Following gene: 30062184

Centisome position: 14.97

GC content: 54.08

Gene sequence:

>564_bases
ATGATGACGACAATTCTGACGATTCATCTTCCTTCAACGCCGGGGCGCGCATGGAATATGCTGGTGGCACTTCAGGCAGG
AAACATCGTCGCCCGTTATGCCAAACTGCATCTCTATGATGCATTTGCCATTCAGGAATCACGCCATGTTGATGCTGGTA
ATGAAATAGCTCCGTTACTGGAGGTGGAAGGGATGAAGGTCGGTCTGATGACCTGTTATGACTTACGCTTTCCAGAGCTG
GCGCTGGCACAGGCTTTACAGGGAGCTGAAATCCTGGTACTTCCTGCCGCCTGGGTTCGTGGGCCGCTCAAAGAGCATCA
CTGGTCAACGTTGCTTGCCGCTCGTGCGCTGGATACCACCTGTTATATGGTGGCGGCGGGGGAGTGCGGGAACAAAAATA
TCGGGCAAAGCCGGATTATAGATCCCTTTGGCGTCACCATTGCGGCAGCGTCAGAAATGCCTGCACTCATTATGGCGGAA
GTGACGCCCGAACGTGTGCGTCAGGTGCGCGCGCAACTGCCCATCTTAAACAACCGTCGCTTTGCGCCGCCGCAATTATT
ATGA

Upstream 100 bases:

>100_bases
GCTGGCGCGCGATGATCATGATGCAGATCTATCGGTTAAATCAGCACAGCTGCTGGAAGGCGAATTCCTCGGACGTTTAC
GGCGAGAAAGTAAACGTAAC

Downstream 100 bases:

>100_bases
TGTTTTTTTACTCGGCGCTTGATTCACCTTGTTACAGATTGCTATTGTGTGCGCGCGTCGAATGACCGTTAATATTCTCT
GGTTTTTAAGGCGCGTTCTG

Product: putative amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MMTTILTIHLPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPILNNRRFAPPQLL

Sequences:

>Translated_187_residues
MMTTILTIHLPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPILNNRRFAPPQLL
>Mature_187_residues
MMTTILTIHLPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL
ALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAE
VTPERVRQVRAQLPILNNRRFAPPQLL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=178, Percent_Identity=39.3258426966292, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI297632348, Length=178, Percent_Identity=39.3258426966292, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI297632350, Length=178, Percent_Identity=39.3258426966292, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI9910460, Length=179, Percent_Identity=28.4916201117318, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1786412, Length=162, Percent_Identity=30.8641975308642, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=181, Percent_Identity=34.8066298342541, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322335, Length=214, Percent_Identity=32.2429906542056, Blast_Score=104, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323383, Length=183, Percent_Identity=31.1475409836066, Blast_Score=96, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355835, Length=167, Percent_Identity=31.7365269461078, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17933642, Length=166, Percent_Identity=33.1325301204819, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 20491; Mature: 20491

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTTILTIHLPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRHVDAGNEIAPLL
CCEEEEEEEECCCCCCCEEEEEEEECCCEEEHHHHHHEEHHEEECCCCCCCCCCCCCCEE
EVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTT
EECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHCCE
CYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRR
EEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
FAPPQLL
CCCCCCC
>Mature Secondary Structure
MMTTILTIHLPSTPGRAWNMLVALQAGNIVARYAKLHLYDAFAIQESRHVDAGNEIAPLL
CCEEEEEEEECCCCCCCEEEEEEEECCCEEEHHHHHHEEHHEEECCCCCCCCCCCCCCEE
EVEGMKVGLMTCYDLRFPELALAQALQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTT
EECCCEEEEEEEECCCCCHHHHHHHHCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHCCE
CYMVAAGECGNKNIGQSRIIDPFGVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRR
EEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECCHHHHHHHHHHCCCCCCCC
FAPPQLL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]