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The map label for this gene is pnuC

Identifier: 30062093

GI number: 30062093

Start: 570181

End: 570900

Strand: Reverse

Name: pnuC

Synonym: S0561

Alternate gene names: 30062093

Gene position: 570900-570181 (Counterclockwise)

Preceding gene: 30062094

Following gene: 30062090

Centisome position: 12.41

GC content: 48.33

Gene sequence:

>720_bases
ATGGATTTTTTTAGTGTGCAGAATATCCTGGTACATATACCAATAGGGGCGGGCGGTTATGATCTCTCATGGATCGAAGC
GGTAGGCACGATCGCCGGGTTGCTGTGTATTGGCCTTGCCAGTCTGGAGAAGATCAGCAACTACTTCTTTGGCCTGATCA
ACGTTACCTTGTTTGGCATTATTTTCTTTCAGATTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCG
AATATTTACGGCTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCC
GAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTT
TGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCCCG
TTCTGGGATTCATGCATGATGGTGTTATCTATCGTGGCAATGATTCTGATGACGCGTAAGTATGTGGAAAACTGGCTGTT
GTGGGTGATTATTAACGTGATTAGCGTCGTTATTTTTGCACTTCAGGGCGTTTACGCCATGTCTCTGGAGTACATCATCC
TGACCTTTATTGCGCTCAACGGCAGCCGGATGTGGATCAACAGCGCACGTGAAAGAGGCTCACGCGCGCTGTCCCATTAA

Upstream 100 bases:

>100_bases
CGAGAGAGGGCGCTGGTGCCGCTCAATCGTATGCTGGATTTTGCGGCTACACTACGTGGATAACGAATAATAAGGCGTAA
CGTTACGCTTTGGGGGAAAG

Downstream 100 bases:

>100_bases
TGGTGATGATGTGAATGGCCAGACACCCCTTCATTCAGGTGGCAGTCTGGCCTATGACAAGGTTGATATTCCATCTGAAT
GGTGGTGTGCTCAATCTGAT

Product: PnuC protein

Products: nicotinamide nucleotide [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEKISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAA
NIYGWYAWSRQTSQNEAELKIRWLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIALNGSRMWINSARERGSRALSH

Sequences:

>Translated_239_residues
MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEKISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAA
NIYGWYAWSRQTSQNEAELKIRWLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIALNGSRMWINSARERGSRALSH
>Mature_239_residues
MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEKISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAA
NIYGWYAWSRQTSQNEAELKIRWLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIALNGSRMWINSARERGSRALSH

Specific function: Required for nicotinamide riboside transport across the inner membrane

COG id: COG3201

COG function: function code H; Nicotinamide mononucleotide transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family

Homologues:

Organism=Escherichia coli, GI1786965, Length=239, Percent_Identity=100, Blast_Score=479, Evalue=1e-137,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNUC_ECOLI (P0AFK2)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X12713
- PIR:   G64810
- RefSeq:   AP_001382.1
- RefSeq:   NP_415272.1
- ProteinModelPortal:   P0AFK2
- STRING:   P0AFK2
- EnsemblBacteria:   EBESCT00000003559
- EnsemblBacteria:   EBESCT00000015591
- GeneID:   945350
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0734
- KEGG:   eco:b0751
- EchoBASE:   EB1651
- EcoGene:   EG11700
- eggNOG:   COG3201
- GeneTree:   EBGT00050000011679
- HOGENOM:   HBG678458
- OMA:   GGGYAMS
- ProtClustDB:   PRK15397
- BioCyc:   EcoCyc:PNUC-MONOMER
- Genevestigator:   P0AFK2
- GO:   GO:0006810
- InterPro:   IPR006419
- TIGRFAMs:   TIGR01528

Pfam domain/function: PF04973 NMN_transporter

EC number: NA

Molecular weight: Translated: 26997; Mature: 26997

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10da646c)-; HASH(0x10e2e4f4)-; HASH(0x111b05a8)-; HASH(0x11142088)-; HASH(0x10c865b8)-; HASH(0x1101b254)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEKISNYFFGLINVTLFGI
CCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIRWLPLPKALSWLAVCVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
GLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFPFWDSCMMVLSIVAMILMTRK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIALNGSRMWINSARERGSRALSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEKISNYFFGLINVTLFGI
CCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIRWLPLPKALSWLAVCVVSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHH
GLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFPFWDSCMMVLSIVAMILMTRK
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIALNGSRMWINSARERGSRALSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide nucleotide [Periplasm] [C]

Specific reaction: nicotinamide nucleotide [Periplasm] = nicotinamide nucleotide [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2841129; 2198247