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The map label for this gene is citE

Identifier: 30062073

GI number: 30062073

Start: 551318

End: 552226

Strand: Reverse

Name: citE

Synonym: S0540

Alternate gene names: 30062073

Gene position: 552226-551318 (Counterclockwise)

Preceding gene: 30062074

Following gene: 30062072

Centisome position: 12.01

GC content: 57.32

Gene sequence:

>909_bases
ATGATTTCCGCTTCGCTGCAACAACGTAAAACTCGCACCCGCCGCAGCATGTTGTTTGTGCCTGGTGCCAATGCCGCGAT
GGTCAGCAACTCCTTCATCTACCCGGCTGATGCCCTGATGTTTGACCTCGAAGACTCCGTGGCATTACGTGAAAAAGACA
CCGCCCGCCGCATGGTTTATCACGCGCTGCAACATCCGCTGTATCGCGATATTGAAACCATTGTGCGTGTCAACGCGCTG
GATTCCGAATGGGGTGTTAACGACCTGGAAGCCGTCGTTCGCGGTGGTGCGGACGTTGTGCGTCTGCCGAAAACCGATAC
CGCTCAGGATGTTCTGGATATTGAAAAAGAGATCCTGCGTATAGAAAAAGCCTGTGGTCGTGAACCCGGCAGCACCGGCC
TGCTGGCGGCGATTGAATCTCCGCTGGGCATTACCCGCGCAGTGGAAATCGCTCACGCTTCCGAGCGTTTGATCGGTATC
GCCCTCGGTGCAGAAGACTATGTGCGCAACCTGCGTACAGAACGCTCCCCGGAAGGAACTGAACTGCTGTTCGCACGCTG
TTCCATTTTGCAGGCCGCGCGCTCTGCGGGTATTCAGGCGTTCGATACCGTCTATTCCGACGCTAACAACGAAGCCGGAT
TCCTGCAAGAAGCCGCCCACATCAAACAGCTGGGCTTTGACGGCAAATCGCTGATCAACCCGCGTCAGATTGATCTGCTG
CACAACCTCTACGCACCGACCCAGAAAGAAGTGGATCACGCCCACCGCGTCGTAGAAGCCGCTGAAGCCGCCGCTCGCGA
AGGCCTCGGCGTGGTTTCCCTGAACGGCAAGATGGTGGACGGTCCGGTTATCGATCGCGCCCGTCTGGTGCTCTCCCGTG
CAGAACTTTCCGGCATCCGCGAAGAATAA

Upstream 100 bases:

>100_bases
TCGATGACAAAGGCGCACTGGACTGCATTTTACATGCACGACTGGAAGCCCTGCTGGCACGCGCCAGCGGTATCCCGGCT
CTGCCATGGGAGGATTGCCA

Downstream 100 bases:

>100_bases
GGCAATCAAAATGACGCAGAAAATTGAACAATCTCAACGACAAGAACGGGTAGCGGCCTGGAATCGTCGCACTGAATGCG
ATCTTGCCGCTTTCCAGAAC

Product: citrate lyase beta chain (acyl lyase subunit)

Products: NA

Alternate protein names: Citrase beta chain; Citrate (pro-3S)-lyase subunit beta; Citryl-CoA lyase subunit [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Sequences:

>Translated_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE
>Mature_302_residues
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVYHALQHPLYRDIETIVRVNAL
DSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIREE

Specific function: Represents a citryl-ACP lyase [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=310, Percent_Identity=25.1612903225806, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI87081764, Length=302, Percent_Identity=99.6688741721854, Blast_Score=608, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI17557458, Length=301, Percent_Identity=27.906976744186, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR006475
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.3.6; =4.1.3.34 [H]

Molecular weight: Translated: 33091; Mature: 33091

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC
>Mature Secondary Structure
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
CCCCHHHHHHHHHHHCEEEECCCCHHHHCCCCCCCCHHHEEECCCCHHHHHHHHHHHHHH
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
HHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHH
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
HHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHEEEEEECHHHHHHHHHHCCCCCHH
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCCHHHHHH
HNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
HHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHHHHHHCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]