Click here to switch to the map view.

The map label for this gene is entD

Identifier: 30062040

GI number: 30062040

Start: 508075

End: 508704

Strand: Reverse

Name: entD

Synonym: S0501

Alternate gene names: 30062040

Gene position: 508704-508075 (Counterclockwise)

Preceding gene: 30062041

Following gene: 30062035

Centisome position: 11.06

GC content: 48.57

Gene sequence:

>630_bases
ATGGTCGATATGAAAACTACGCATACCTCCCTCCCCTTTGCCGGACATACGCTGCATTTTGTTGAGTTCGATCCGGCGAA
TTTTTGTGAGCAGGATTTACTCTGGCTGCCGCACTACGCACAACTGCAACACGCTGGACGTAAACGTAAAACAGAGCATT
TAGCCGGACGGATCGCTGCTGTTTATGCCTTGCGGGAATATGGCTATAAATGTGTGCCCGCAATCGGCGAGCTACGCCAA
CCGGTGTGGCCTGCGGAGGTATACGGCAGTATTAGCCACTGCGGGGCTACGGCATTGGCCGTGGTTTCCCGTCAACCGAT
TGGCGTTGATATCGAAGAAATTTTTTCTGCGCAAACAGCGACCGAATTGACAGACAACATTATTACACCAGCAGAACACG
AGCGACTCGCAGACTGCGGTTTAGCCTTTTCTCTGGCGCTGACACTTGCATTTTCCGCCAAAGAGAGCGCATTTAAGGCA
AGTGAGATCCAAACTGATGCAGGTTTTCTGGACTATCAGATAATTAGCTGGAATAAACAGCAGGTCATCATTCATCGTGA
GAATGAGATGTTTGCTGTGCACTGGCAGATAAAAGAAAAAATAGTCATAACTCTGTGCCAACACGATTAA

Upstream 100 bases:

>100_bases
CGGCCTACAAAGATCTTGCCAATTCAATATATTGCAGGATCACGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGC
GTTTGTCATCAGTCTCGAAT

Downstream 100 bases:

>100_bases
TTGACAACATCTGGTACGATTCGCCCGCAGCCATCACTGACTGCGGGCGAAAGTGTAAAGCAGGTGCCTTACCACTCTGA
CCTGACAACCGGATATGCGG

Product: phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex

Products: NA

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKCVPAIGELRQ
PVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKA
SEIQTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD

Sequences:

>Translated_209_residues
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKCVPAIGELRQ
PVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKA
SEIQTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD
>Mature_209_residues
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKCVPAIGELRQ
PVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKA
SEIQTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family

Homologues:

Organism=Escherichia coli, GI1786797, Length=209, Percent_Identity=98.0861244019139, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ENTD_ECO57 (P0A3B9)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85557
- PIR:   F90706
- RefSeq:   NP_286309.1
- RefSeq:   NP_308649.1
- ProteinModelPortal:   P0A3B9
- EnsemblBacteria:   EBESCT00000027745
- EnsemblBacteria:   EBESCT00000058618
- GeneID:   916981
- GeneID:   957588
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0723
- KEGG:   ecs:ECs0622
- GeneTree:   EBGT00050000011324
- HOGENOM:   HBG739719
- OMA:   MKTTHTA
- ProtClustDB:   PRK10251
- BioCyc:   ECOL83334:ECS0622-MONOMER
- InterPro:   IPR008278
- InterPro:   IPR003542
- PRINTS:   PR01399

Pfam domain/function: PF01648 ACPS; SSF56214 4-PPT_transf

EC number: 2.7.8.-

Molecular weight: Translated: 23477; Mature: 23477

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAA
CCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTA
HHHHHHCCCEECCCHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEIQTDAGFLDYQIISWNKQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEECCCE
QVIIHRENEMFAVHWQIKEKIVITLCQHD
EEEEEECCCEEEEEEEECCEEEEEEECCC
>Mature Secondary Structure
MVDMKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGRKRKTEHLAGRIAA
CCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
VYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQPIGVDIEEIFSAQTA
HHHHHHCCCEECCCHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEIQTDAGFLDYQIISWNKQ
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEEEEECCCE
QVIIHRENEMFAVHWQIKEKIVITLCQHD
EEEEEECCCEEEEEEEECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796