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The map label for this gene is ybdG

Identifier: 30062031

GI number: 30062031

Start: 501433

End: 502680

Strand: Reverse

Name: ybdG

Synonym: S0492

Alternate gene names: 30062031

Gene position: 502680-501433 (Counterclockwise)

Preceding gene: 30062032

Following gene: 30062028

Centisome position: 10.93

GC content: 47.52

Gene sequence:

>1248_bases
ATGCAGGATTTAATATCCCAGGTTGAAGATTTAGCGGGTATTGAGATCGATCACACCACCTCGATGGTGATGATTTTCGG
TATTATTTTTCTGACCGCCGTCGTGGTGCATATTATTTTGCATTGGGTGGTACTGCGGACCTTCGAAAAACGTGCCATCG
CCAGTTCACGGCTTTGGTTGCAAATCATTACCCAGAATAAACTCTTCCACCGTTTAGCTTTTACCCTGCAGGGGATTATC
GTCAATATTCAGGCGGTATTCTGGCTGCAAAAAGGCACCGAAGCGGCAGATATTCTGACTACCTGCGCGCAGTTGTGGAT
CATGATGTATGCGCTGCTTTCAGTCTTCTCGTTGCTGGATGTTATTTTGAATCTGGCGCAGAAATTCCCGGCGGCATCTC
AGTTACCGCTGAAAGGGATATTTCAGGGGATTAAACTGATCGGCGCAATCCTGGTCGGCATTTTGATGATCTCGCTGCTG
ATTGGTCAGTCGCCAGCGATTCTAATCAGCGGTCTTGGTGCAATGGCTGCCGTGCTGATGTTGGTATTTAAAGATCCGAT
TCTTGGTCTGGTGGCAGGTATTCAGCTTTCCGCGAACGATATGCTGAAACTGGGCGACTGGCTGGAAATGCCGAAATACG
GCGCGGATGGCGCGGTGATCGATATTGGGTTAACCACCGTCAAAGTGCGTAACTGGGACAATACCATTACCACTATTCCC
ACCTGGTCTCTGGTTTCTGACTCCTTTAAAAACTGGAGCGGGATGTCAGCATCTGGCGGGCGACGTATTAAGCGCAGTAT
CAGTATTGATGTCACCAGTATTCGTTTTCTTGATGAAGACGAAATGCAACGTCTGAATAAAGCGCATTTGTTAAAGCCTT
ATTTAACCAGCCGCCATCAGGAAATTAATGAGTGGAATCGCCAGCAAGGTTCTACGGAGTCGGTATTAAACCTGCGCCGA
ATGACCAATATTGGAACCTTTCGTGCCTATCTGAACGAATATCTGCGTAACCATCCGCGGATTCGTAAAGATATGACCTT
AATGGTACGCCAACTGGCTCCAGGTGATAACGGTTTACCGCTCGAGATCTACGCGTTTACTAACACCGTGGTGTGGCTGG
AATATGAAAGCATTCAGGCTGATATATTCGATCACATATTTGCGATTGTCGAAGAGTTTGGTCTGCGACTTCATCAGTCG
CCAACCGGCAATGATATTCGCTCTCTGGCGGGTGCATTTAAGCAGTAA

Upstream 100 bases:

>100_bases
TTGCCGGGCATCTGCCCGGCTATTTCCCCTCAGATTCTCCTGATTTGCATAACCCTGTTTCAGCCGTCATCATAGGCTGC
TGTTGTATAAAGGAGACGTT

Downstream 100 bases:

>100_bases
TTAAAAAAACCGCTCTCATCGAATGGATGAGAGCGGACATCGTTTTGTCGGATGTAGCGTGAATGCCTTATTTCCGACGC
AGCGTTTTAAATGCCACAAA

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGII
VNIQAVFWLQKGTEAADILTTCAQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIP
TWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRR
MTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEEFGLRLHQS
PTGNDIRSLAGAFKQ

Sequences:

>Translated_415_residues
MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGII
VNIQAVFWLQKGTEAADILTTCAQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIP
TWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRR
MTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEEFGLRLHQS
PTGNDIRSLAGAFKQ
>Mature_415_residues
MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGII
VNIQAVFWLQKGTEAADILTTCAQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIP
TWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRR
MTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEEFGLRLHQS
PTGNDIRSLAGAFKQ

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family

Homologues:

Organism=Escherichia coli, GI1786791, Length=415, Percent_Identity=100, Blast_Score=842, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCM_ECOLI (P0AAT4)

Other databases:

- EMBL:   U82598
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   U07860
- EMBL:   D25414
- PIR:   G64790
- RefSeq:   AP_001222.1
- RefSeq:   NP_415109.1
- ProteinModelPortal:   P0AAT4
- STRING:   P0AAT4
- EnsemblBacteria:   EBESCT00000002760
- EnsemblBacteria:   EBESCT00000014593
- GeneID:   946243
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0566
- KEGG:   eco:b0577
- EchoBASE:   EB2504
- EcoGene:   EG12620
- eggNOG:   COG0668
- GeneTree:   EBGT00050000009774
- HOGENOM:   HBG664382
- OMA:   LPLRGIF
- ProtClustDB:   CLSK873917
- BioCyc:   EcoCyc:G6323-MONOMER
- Genevestigator:   P0AAT4
- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685

Pfam domain/function: PF00924 MS_channel; SSF82689 MscC_channel_C; SSF50182 Sm_like_riboprot

EC number: NA

Molecular weight: Translated: 46603; Mature: 46603

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfbedcf0)-; HASH(0x10c8111c)-; HASH(0xfa8953c)-; HASH(0xf7bd5b0)-; HASH(0xf698168)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTCAQLWIMMYALLSVFSLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLLIGQSPAILISGLGAMAAVLM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIP
HHHHCHHHHHHHCCEECCCHHHHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCCEEECC
TWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQ
CHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
EINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LEIYAFTNTVVWLEYESIQADIFDHIFAIVEEFGLRLHQSPTGNDIRSLAGAFKQ
EEEEEEECEEEEEEEHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCH
>Mature Secondary Structure
MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVVLRTFEKRAIASSRLWL
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTCAQLWIMMYALLSVFSLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLLIGQSPAILISGLGAMAAVLM
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGAVIDIGLTTVKVRNWDNTITTIP
HHHHCHHHHHHHCCEECCCHHHHHHHHHHCCCCCCCCCEEEECEEEEEEECCCCCEEECC
TWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRFLDEDEMQRLNKAHLLKPYLTSRHQ
CHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
EINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEYLRNHPRIRKDMTLMVRQLAPGDNGLP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCC
LEIYAFTNTVVWLEYESIQADIFDHIFAIVEEFGLRLHQSPTGNDIRSLAGAFKQ
EEEEEEECEEEEEEEHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889