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The map label for this gene is insD1 [C]

Identifier: 30062027

GI number: 30062027

Start: 494699

End: 495490

Strand: Reverse

Name: insD1 [C]

Synonym: S0487

Alternate gene names: 30062027

Gene position: 495490-494699 (Counterclockwise)

Preceding gene: 30062028

Following gene: 30062026

Centisome position: 10.77

GC content: 54.29

Gene sequence:

>792_bases
ATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTCTCCGTATACACCATGTTATCGGAGAGCTGCCCAC
GTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAACTTGATGGTATGCCTGCGATCAATGCCAAACGTG
TTTACCGGCTCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACCTGCTGTATCACCATCGAAACGGGCACATACAGGC
AGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGTTCGAGTTCTGCTGTGATAACGGAGAGAGACTGCG
TGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGGGCGAGGACTACCGGCGGCTTCAACAGTGAAACAG
TACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGATCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGAT
AATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGATGTTGGGACTTGAACCGAAGAACACGGCGGTGCG
GAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAG
ACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTAT
CGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAG

Upstream 100 bases:

>100_bases
CGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCAGTTGCACGTCATTC
TCAGACGAACCGATGACTGA

Downstream 100 bases:

>100_bases
GGGCAAATCCATCACCAGTTAACGTACAACCACTTACAAAGACGAAGATCGCCGACTGTTCTATCGCTATACTTTGATTG
TTTGCAGCCGTGTTTTTGTT

Product: insertion element IS2 transposase InsD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVSPSKRAHTG
RVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWARTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTD
NGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
RSPREYLRQRACNGLSDNRCLEI

Sequences:

>Translated_263_residues
MDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVSPSKRAHTG
RVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWARTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTD
NGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
RSPREYLRQRACNGLSDNRCLEI
>Mature_263_residues
MDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQNALLLERKPAVSPSKRAHTG
RVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWARTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTD
NGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
RSPREYLRQRACNGLSDNRCLEI

Specific function: Involved in the transposition of the insertion sequence is2 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790724, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1789424, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1789224, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1788305, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1787669, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1786558, Length=263, Percent_Identity=98.8593155893536, Blast_Score=544, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 30098; Mature: 30098

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
NALLLERKPAVSPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREAL
CHHHEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHH
HWARTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEECHHHHHHHHHHCCC
PKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RSPREYLRQRACNGLSDNRCLEI
CCHHHHHHHHHCCCCCCCCEECC
>Mature Secondary Structure
MDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRLMRQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
NALLLERKPAVSPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREAL
CHHHEECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCCCHHHH
HWARTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLE
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCEEECHHHHHHHHHHCCC
PKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
RSPREYLRQRACNGLSDNRCLEI
CCHHHHHHHHHCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA