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The map label for this gene is sbcD

Identifier: 30061894

GI number: 30061894

Start: 344392

End: 345594

Strand: Reverse

Name: sbcD

Synonym: S0343

Alternate gene names: 30061894

Gene position: 345594-344392 (Counterclockwise)

Preceding gene: 30061906

Following gene: 30061893

Centisome position: 7.51

GC content: 53.2

Gene sequence:

>1203_bases
ATGCGCATCCTTCACACCTCAGACTGGCATCTCGGCCAGAACTTCTACAGTAAAAGCCGCGAAGCTGAACATCAGGCTTT
TCTTGACTGGCTGCTGGAGACAGCACAAACCCATCAGGTGGATGCGATTATTGTTGCTGGTGATGTTTTCGATACCGGCT
CGCCGCCCAGTTACGCCCGCACGTTATACAACCGTTTTGTTGTCAATTTACAGCAAACTGGCTGTCATCTGGTGGTACTG
GCAGGAAACCATGACTCGGTCGCCACGCTGAATGAATCGCGCGATATCATGGCGTTCCTCAATACAACCGTGGTCGCCAG
CGCCGGACATGCGCCGCAAATCTTGCCTCGTCGCGACGGGACGCCAGGCGCAGTGCTGTGCCCCATTCCGTTTTTACGCC
CGCGTGACATTATTTCCAGCCAGGCGGGGCTTAACGGTATTGAAAAACAGCAGCATTTACTGGCAGCGATTACCGATTAT
TACCAACAACACTATGCCGATGCCTGCAAACTGCGCGGCGATCAGCCTCTGCCCATCATCGCCACGGGACATTTAACGAC
CGTGGGTTCCAGTAAAAGTGACGCCGTGCGTGACATTTATATTGGCACGCTGGACGCGTTTCCGGCACAAAACTTTCCAC
CAGCCGACTACATCGCGCTCGGGCATATTCACCGCGCACAGATTATTGGCGGCATGGAACATGTTCGCTATTGCGGTTCC
CCCATTCCACTGAGTTTTGATGAATGCGGTAAGAGTAAATATGTCCATCTGGTGACATTTTCAAACGGCAAATTAGAGAG
CGTGGAAAACCTGAACGTACCGGTAACTCAACCCATGGCGGTGCTGAAAGGCGATCTGGCGTCGATTACCGCACAGCTGG
AACAGTGGCGCGATGTATCGCAGGAGCCACCTGTCTGGCTGGATATCGAAATCACTACTGATGAGTATCTGCATGATATT
CAGCGCAAAATCCAGGCATTAACCGAATCATTGCCTGTCGAAGTATTGCTGGTACGTCGGAGTCGTGAACAGCGCGAGCG
TGTGTTAGCCAGCCAACAGCGTGAAACCCTCAGCGAACTCAGCGTCGAAGAGGTGTTCAATCGCCGTCTGGCACTGGAAG
ACCTGGATGAATCGCAGCAGCAACGTCTGCAGCATCTTTTCACCACGACGTTGCGTACCCTCGCCGGAGAACACGAAGCA
TGA

Upstream 100 bases:

>100_bases
CACATATCTTCAGGTTATTGATTTCCGTGGCGCAGAAAAAAGCAAATGGCACATCTGTTTGGGTATAATCGCGACCATGC
TTTTTCGCCAGGGAACCGTT

Downstream 100 bases:

>100_bases
AAATTCTCAGCCTGCGCCTGAAAAACCTGAACTCATTAAAAGGCGAATGGAAGATTGATTTCACCCGCGAGCCGTTCGCC
AGCAACGGGCTGTTTGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLRTLAGEHEA

Sequences:

>Translated_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLRTLAGEHEA
>Mature_400_residues
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVL
AGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDI
QRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLRTLAGEHEA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=400, Percent_Identity=99, Blast_Score=821, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 44721; Mature: 44721

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEECCCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEDLDESQQQRLQHLFTTTLRTLAGEHEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHH
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
HHHHHHHHEEECCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHEECCCCCCCCCCCCCC
TPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
CCCEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
ATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
EECCEEECCCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCC
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVS
CCCCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
CCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
SVEEVFNRRLALEDLDESQQQRLQHLFTTTLRTLAGEHEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]