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The map label for this gene is proC

Identifier: 30061882

GI number: 30061882

Start: 333212

End: 334021

Strand: Reverse

Name: proC

Synonym: S0330

Alternate gene names: 30061882

Gene position: 334021-333212 (Counterclockwise)

Preceding gene: 30061890

Following gene: 30061880

Centisome position: 7.26

GC content: 54.81

Gene sequence:

>810_bases
ATGGAAAAGAAAATCGGTTTTATTGGCTGCGGCAATATGGGAAAAGCCATTCTCGGCGGTCTGATTGCCAGCGGTCAGGT
GCTTCCAGGGCAAATCTGGGTATACACCCCCTCCCCGGATAAAGTCGCCGCCCTGCATGACCAGTTCGGCATCAACGCCG
CAGAATCGGCGCAAGAAGTGGCGCAAATCGCCGACATCATTTTTGCTGCCGTTAAACCTGGCATCATGATTAAAGTGCTT
AGCGAAATCACCTCCAGCCTGAATAAAGACTCTCTGGTCGTTTCTATTGCTGCAGGTGTCACGCTCGACCAGCTTGCCCG
CGCGCTGGGCCATGACCGGAAAATTATCCGCGCCATGCCGAACACTCCCGCGCTGGTTAATGCCGGGATGACCTCCGTAA
CGCCAAACGCGCTGGTAACCCCAGAAGATACCGCTGATGTGCTGAATATTTTCCGCTGCTTTGGCGAAGCGGAAGTAATT
GCTGAGCCGATGATCCACCCGGTGGTCGGTGTGAGCGGTTCTTCGCCAGCCTACGTATTTATGTTTATCGAAGCGATGGC
CGACGCCGCCGTGCTGGGCGGGATGCCACGCGCCCAGGCGTATAAATTTGCCGCTCAGGCGGTAATGGGTTCCGCAAAAA
TGGTGCTGGAAACGGGAGAACATCCGGGGGCACTGAAAGATATGGTCTGCTCACCGGGAGGCACCACCATTGAAGCGGTA
CGCGTACTGGAAGAGAAAGGCTTCCGTGCCGCAGTGATCGAAGCGATGACGAAGTGTATGGAAAAATCAGAAAAACTCAG
CAAATCCTGA

Upstream 100 bases:

>100_bases
TAATCCTCTATTGTGTCGCGCTTTTGCCTTCCGGCATAGTTCTGTTTATGCTTCTGCCAGCGATTATCAAAACAATGAAT
TTCACGGCAGGAGTGAGGCA

Downstream 100 bases:

>100_bases
TGACTTTCGCCGGACGTCAGGCCGCCACTTCGGTGCGGTTACGTCCGGCTTTCTTTGCTTTGTAAAGCGCCAAATCTGCC
GATTTCAACCACTCACGATA

Product: pyrroline-5-carboxylate reductase

Products: NA

Alternate protein names: P5C reductase; P5CR

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Sequences:

>Translated_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
>Mature_269_residues
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVL
SEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS

Specific function: Proline biosynthesis; third (last) step. [C]

COG id: COG0345

COG function: function code E; Pyrroline-5-carboxylate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrroline-5-carboxylate reductase family

Homologues:

Organism=Homo sapiens, GI24797097, Length=266, Percent_Identity=37.9699248120301, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI24797095, Length=266, Percent_Identity=37.9699248120301, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI21361454, Length=266, Percent_Identity=37.9699248120301, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI198041662, Length=266, Percent_Identity=34.5864661654135, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1786585, Length=269, Percent_Identity=100, Blast_Score=545, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI17569021, Length=264, Percent_Identity=40.1515151515151, Blast_Score=185, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17540664, Length=285, Percent_Identity=37.1929824561403, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6320861, Length=272, Percent_Identity=29.0441176470588, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648116, Length=277, Percent_Identity=38.2671480144404, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21358587, Length=265, Percent_Identity=39.2452830188679, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24647700, Length=169, Percent_Identity=41.4201183431953, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): P5CR_ECOLI (P0A9L8)

Other databases:

- EMBL:   J01665
- EMBL:   U73857
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   A00385
- RefSeq:   AP_001037.1
- RefSeq:   NP_414920.1
- ProteinModelPortal:   P0A9L8
- SMR:   P0A9L8
- DIP:   DIP-47863N
- STRING:   P0A9L8
- EnsemblBacteria:   EBESCT00000003053
- EnsemblBacteria:   EBESCT00000014492
- GeneID:   945034
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0377
- KEGG:   eco:b0386
- EchoBASE:   EB0762
- EcoGene:   EG10769
- eggNOG:   COG0345
- GeneTree:   EBGT00050000011553
- HOGENOM:   HBG726602
- OMA:   RTFNEHQ
- ProtClustDB:   PRK11880
- BioCyc:   EcoCyc:PYRROLINECARBREDUCT-MONOMER
- BioCyc:   MetaCyc:PYRROLINECARBREDUCT-MONOMER
- Genevestigator:   P0A9L8
- GO:   GO:0005737
- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR004455
- InterPro:   IPR000304
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR11645
- PIRSF:   PIRSF000193
- TIGRFAMs:   TIGR00112

Pfam domain/function: PF03807 F420_oxidored; SSF48179 6DGDH_C_like

EC number: =1.5.1.2

Molecular weight: Translated: 28145; Mature: 28145

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00521 P5CR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAALHDQFGINAAESAQEV
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHH
AQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTLDQLARALGHDRKIIRAMP
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCHHHHHCC
NTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVIAEPMIHPVVGVSGSSPAYVF
CCCCEEECCCCCCCCCCEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHH
MFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLETGEHPGALKDMVCSPGGTTIEAV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHEEECCCCCCHHHHHHCCCCCCHHHHH
RVLEEKGFRAAVIEAMTKCMEKSEKLSKS
HHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6296787; 9278503