Click here to switch to the map view.

The map label for this gene is insF1 [H]

Identifier: 30061812

GI number: 30061812

Start: 265695

End: 266507

Strand: Reverse

Name: insF1 [H]

Synonym: S0262

Alternate gene names: 30061812

Gene position: 266507-265695 (Counterclockwise)

Preceding gene: 30061813

Following gene: 30061810

Centisome position: 5.79

GC content: 50.43

Gene sequence:

>813_bases
GTGCTGGATGTTCATCCCAGTGGTTTTTACGCCTGGCTTCAGCAGCCGCATTCACAACGCCATCAGGCAGACCTGAGACT
GACAGGACAGATTAAACAGTTCTGGCTGGAATCGGGATGCGTCTATGGTTATCGCAAAATCCATCTGGATCTGCGTGACA
GCGGACAACAGTGCGGAGTAAACAGAGTCTGGAGACTGATGAAACGTGTCGGAATAAAGGCTCAGGTCGGATACCGAAGC
CCGCGGGCACGTAAAGGCGAGGCCAGTATCGTGTCACCCAACAGGCTCCAGCGACAGTTCAATCCGGATGCTCCTGATGA
GCGTTGGGTAACGGACATAACCTACATCAGGACCCACGAAGGCTGGCTGTATCTTGCCGTTGTTGTTGATCTGTTCTCAC
GCAAAATTATCGGCTGGTCCATGCAATCCCGGATGACAAAGGACATTGTCCTGAACGCACTGCTGATGGCTGTATGGCGG
CGTAATCCCGAAAAACAGGTGCTGGTTCATTCGGATCAGGGCAGTCAGTACACAAGCCATGAGTGGCAGTCGTTCCTGAA
ATCACACGGCCTGGAGGGTAGCATGAGCCGTCGCGGTAACTGCCATGATAATGCGGTTGCAGAAAGTTTTTTCCAGTTGT
TGAAACGTGAACGGATAAAGAAAAAGATCTACGGAACGCGGGAAGAAGCCCGCAGTGATATTTTTGATTACATCGAAATG
TTTTATAACAGTAAGCGTCGGCATGGTTCTAGCGAACAGATGTCACCGACAGAATATGAAAACCAGTATTATCAACGGCT
CGGAAGTGTCTAG

Upstream 100 bases:

>100_bases
TATTAAAAAAAGCCGCGGCGTACTTCGCAAAGCTGTCCGACTGAGGTACGCCTTTATCCGTGACAACTCCTGTTGCTGGC
CTGTTCGCCTGCTCTGTCGG

Downstream 100 bases:

>100_bases
ATTATCCGTGGCGATTCATAGCTTGAGCTACTCTCACTTCAACAACGACCTGTCTGCAACCATGAGCAACAGTACTTACG
TTGACGGTAGCACCAACTCC

Product: ISEhe3 orfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRS
PRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWR
RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEM
FYNSKRRHGSSEQMSPTEYENQYYQRLGSV

Sequences:

>Translated_270_residues
MLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRS
PRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWR
RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEM
FYNSKRRHGSSEQMSPTEYENQYYQRLGSV
>Mature_270_residues
MLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRS
PRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWR
RNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEM
FYNSKRRHGSSEQMSPTEYENQYYQRLGSV

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=226, Percent_Identity=37.1681415929204, Blast_Score=143, Evalue=2e-34,
Organism=Escherichia coli, GI1788405, Length=265, Percent_Identity=41.5094339622642, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1787263, Length=265, Percent_Identity=41.5094339622642, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1786753, Length=265, Percent_Identity=41.5094339622642, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1786570, Length=265, Percent_Identity=41.5094339622642, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1786490, Length=265, Percent_Identity=41.5094339622642, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1789981, Length=258, Percent_Identity=37.5968992248062, Blast_Score=174, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31807; Mature: 31807

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGV
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEEEECCCCCCCCH
NRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHE
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHEEEEECC
GWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYTSH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHH
EWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEM
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FYNSKRRHGSSEQMSPTEYENQYYQRLGSV
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGV
CCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEEEEEECCCCCCCCH
NRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHE
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHEEEEECC
GWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYTSH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHH
EWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEM
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FYNSKRRHGSSEQMSPTEYENQYYQRLGSV
HHHHHHCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]