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The map label for this gene is speE

Identifier: 30061686

GI number: 30061686

Start: 133335

End: 134201

Strand: Reverse

Name: speE

Synonym: S0120

Alternate gene names: 30061686

Gene position: 134201-133335 (Counterclockwise)

Preceding gene: 30061687

Following gene: 30061685

Centisome position: 2.92

GC content: 50.98

Gene sequence:

>867_bases
ATGGCCGAAAAAAAACAGTGGCATGAAACGCTACACGACCAGTTTGGGCAGTACTTTGCGGTAGATAACGTTCTGTATCA
TGAAAAGACCGATCACCAGGATCTGATCATTTTTGAGAACGCTGCATTTGGTCGCGTAATGGCGCTGGATGGCGTAGTAC
AAACCACCGAGCGCGACGAGTTTATCTATCATGAGATGATGACCCATGTTCCGCTACTGGCCCACGGTCACGCGAAACAT
GTGCTGATTATCGGCGGCGGCGACGGTGCCATGCTGCGTGAAGTAACCCGACATAAAAACGTTGAGTCAATCACGATGGT
GGAAATCGATGCGGGTGTCGTGTCGTTCTGCCGTCAGTATCTGCCCAACCATAACGCCGGTAGCTACGACGATCCGCGCT
TTCAACTAGTGATCGACGATGGCGTCAATTTCGTTAATCAAACCAGCCAGACCTTTGATGTCATTATCTCCGACTGCACC
GATCCTATCGGTCCCGGCGAAAGCCTGTTCACCTCAGCATTTTATGAAGGCTGCAAACGTTGCCTGAATCCTGGCGGTAT
CTTCGTCGCACAAAACGGCGTCTGCTTTTTACAGCAGGAAGAAGCCATCGACAGCCATCGCAAACTCAGCCATTACTTCA
GCGACGTTGGCTTTTATCAGGCGGCGATCCCGACCTATTACGGCGGTATCATGACTTTTGCATGGGCGACAGATAACGAC
GCCTTACGCCATCTCTCAACCGAAATTATTCAGGCGCGTTTTCTCGCTTCTGGCCTGAAATGCCGTTATTACAATCCGGC
AATCCATACGGCAGCTTTTGCCTTACCTCAGTATCTGCAAGACGCACTGGCTTCACAGCCGTCCTAA

Upstream 100 bases:

>100_bases
GATACCTTTTTGTAGAAATATTGACCGTGCATCCGCGGTCAATGTTAGCTATTATGTTGCGCCCTTTTTTTACGGGTGTT
AACAAAGGAGGTATCAACCC

Downstream 100 bases:

>100_bases
GGAGAAGATAAGAAATTGAAAAAACTGAAACTGCATGGCTTTAATAATCTGACCAAAAGTCTGAGTTTTTGTATTTACGA
TATCTGCTACGCCAAAACTG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALPQYLQDALASQPS

Sequences:

>Translated_288_residues
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKH
VLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
ALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALPQYLQDALASQPS
>Mature_287_residues
AEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHV
LIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCTD
PIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDNDA
LRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALPQYLQDALASQPS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=286, Percent_Identity=36.3636363636364, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1786312, Length=288, Percent_Identity=99.6527777777778, Blast_Score=603, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI25154106, Length=279, Percent_Identity=40.5017921146953, Blast_Score=194, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI212645616, Length=223, Percent_Identity=42.6008968609865, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=38.8111888111888, Blast_Score=209, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6325326, Length=271, Percent_Identity=36.5313653136531, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24645443, Length=267, Percent_Identity=37.4531835205993, Blast_Score=191, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=35.3488372093023, Blast_Score=145, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32322; Mature: 32190

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALPQYLQDALASQPS
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFGRVMALDGVVQTTERDE
CCHHHHHHHHHHHCCCEEECCCEEECCCCCCCCEEEEECCCCCEEEECCCHHHCCCCHH
FIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVESITMVEIDAGVVSFCRQY
HHHHHHHHHCCCEECCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCTDPIGPGESLFTSAFYEGCKR
CCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGFYQAAIPTYYGGIMTFAWATDND
HCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALPQYLQDALASQPS
HHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA