Definition Shigella flexneri 2a str. 2457T, complete genome.
Accession NC_004741
Length 4,599,354

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The map label for this gene is hofC

Identifier: 30061673

GI number: 30061673

Start: 112511

End: 113713

Strand: Reverse

Name: hofC

Synonym: S0105

Alternate gene names: 30061673

Gene position: 113713-112511 (Counterclockwise)

Preceding gene: 30061674

Following gene: 30061672

Centisome position: 2.47

GC content: 52.54

Gene sequence:

>1203_bases
ATGGCAAGTAAGCAACTCTGGCGCTGGCATGGCATAACCGGCGACGGCAATGCGCAAGATGGGATGCTATGGGCAGAGAG
CCGTACTTTACTGCTTATGGCACTACAGCAACAGATGGTTACCCCACTAAGCCTGAAGCGAATCGCCATCAATTCTGCGC
AGTGGCGAGGAGATAAAAGCGCGGAAGTCATTCATCAACTGGCGACGCTACTTAAAGCCGGGTTAACGCTTTCTGAAGGG
CTGGCTCTGCTGGCGGAACAGCATCCCAGTAAGCAATGGCAAGCGTTGCTGCAATCGCTGGCGCACGATCTCGAACAGGG
CATTGCTTTTTCCAATGCCTTATTACCCTGGTCAGAGGCATTTCCGCCGCTCTATCAGGCGATGATCCGCACGGGTGAAC
TGACCGGTAAGCTGGATGAATGCTGCTTTGAACTGGCGCGTCAGCAAAAATCCCAGCGTCAGTTGACCGACAAAGTGAAA
TCAGCGTTGCGTTATCCCATCATCATTTTAGCGATGGCAATCATGGTGGTTGTGGCAATGCTGCATTTTGTTCTGCCGGA
GTTTGCCGCTATCTATAAGACCTTCAACACCCCACTACCGGCACTAACGCAGGGGATCATGACGCTGGCAGACTTTAGTG
GCGAATGGGGCTGGCTGCTGGTGTTGTTCGGCTTTCTGCTGGCGATAGCCAATAAGTTGCTGATGCGCCGCCCGACCTGG
CTTATCGCGCGGCAGAAATTGCTGTTACGCATCCCGATTATGGGTTCACTGATGAGGGGACAAAAACTCACGCAGATCTT
TACGATTCTGGCGCTGACACAAAGTGCAGGCATTACTTTTTTACAGGGCGTAGAGAGCGTCAGAGAAACAATGCGCTGCC
CGTACTGGGTGCAACTTCTGACACAAATCCAGCACGATATCAGTAACGGTCATCCCATCTGGCTGGCGCTAAAAAATGCC
GGTGAGTTTAGCCCGCTCTGTTTGCAATTAGTGAGAACAGGAGAGGCATCCGGCTCGCTGGACCTCATGTTAGACAACCT
CGCCCATCATCATCGGGATAACACAATGGCGCTGGCGGATAACCTCGCAGCCTTACTGGAACCGGCGTTGCTGATCATAA
CGGGAGGAATTATCGGTACGCTGGTGGTGGCAATGTATCTGCCAATTTTCCATTTAGGCGATGCGATGAGTGGGATGGGA
TAA

Upstream 100 bases:

>100_bases
CGACAGGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAAT
CCGCGTACTGGGGATGCCGC

Downstream 100 bases:

>100_bases
CGCTGGCGTGTTGCTCCACGCCAGCGTTGGGAGATTACAGGTTGTTGAAGATGCGGTTTTCTTGTTCCAGCACACGAATA
AACGTGGTGCGCTTGGTCAG

Product: type IV pilin biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEG
LALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGWLLVLFGFLLAIANKLLMRRPTW
LIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNA
GEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG

Sequences:

>Translated_400_residues
MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEG
LALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGWLLVLFGFLLAIANKLLMRRPTW
LIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNA
GEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG
>Mature_399_residues
ASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGL
ALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVKS
ALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGWLLVLFGFLLAIANKLLMRRPTWL
IARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAG
EFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG

Specific function: Unknown

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1786295, Length=400, Percent_Identity=98.25, Blast_Score=796, Evalue=0.0,
Organism=Escherichia coli, GI1789724, Length=400, Percent_Identity=25.5, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44346; Mature: 44215

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKS
CCCCCCHHEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCHHCCCCCH
AEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
FPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVKSALRYPIIILAMAIMVVVAM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGWLLVLFGFLLAIANKLLMRRPTW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
LIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLL
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH
TQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALAD
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
NLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLSLKRIAINSAQWRGDKS
CCCCCHHEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHCHHHHHHHHHCCHHCCCCCH
AEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAHDLEQGIAFSNALLPWSEA
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
FPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVKSALRYPIIILAMAIMVVVAM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGWLLVLFGFLLAIANKLLMRRPTW
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
LIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSAGITFLQGVESVRETMRCPYWVQLL
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHH
TQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTGEASGSLDLMLDNLAHHHRDNTMALAD
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHH
NLAALLEPALLIITGGIIGTLVVAMYLPIFHLGDAMSGMG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7959070; 8202364; 9278503; 2904262 [H]