| Definition | Shigella flexneri 2a str. 2457T, complete genome. |
|---|---|
| Accession | NC_004741 |
| Length | 4,599,354 |
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The map label for this gene is imp
Identifier: 30061621
GI number: 30061621
Start: 53559
End: 55913
Strand: Reverse
Name: imp
Synonym: S0053
Alternate gene names: 30061621
Gene position: 55913-53559 (Counterclockwise)
Preceding gene: 30061623
Following gene: 30061620
Centisome position: 1.22
GC content: 51.38
Gene sequence:
>2355_bases ATGAAAAAACGTATCCCCACTCTCCTGGCCACCATGATTGCCACCGCCCTTTATAGTCAACAGGGACTGGCAGCCGACCT CGCCTCACAGTGCATGTTGGGCGTGCCAAGCTATGACCGTCCTCTGGTACAGGGCGATACCAATGACTTACCCGTGACTA TCAATGCTGACCACGCGAAAGGGGACTACCCGGATGACGCCGTGTTTACTGGCAGCGTGGATATCATGCAGGGTAACAGC CGTCTGCAGGCCGACGAAGTGCAGCTCCATCAAAAAGAGGCACCAGGACAACCGGAGCCGGTACGTACCGTTGATGCGCT CGGTAATGTCCATTACGACGATAACCAGGTGATCCTCAAAGGGCCGAAAGGCTGGGCGAATCTGAACACCAAAGATACCA ACGTCTGGGAAGGTGATTACCAGATGGTGGGTCGCCAGGGTCGCGGTAAAGCGGACCTGATGAAACAACGTGGCGAAAAC CGCTATACCATTCTGGATAACGGTAGCTTTACCTCCTGTCTGCCGGGTTCTGACACCTGGAGCGTGGTAGGTAGCGAAAT TATTCATGACCGCGAAGAACAAGTTGCTGAGATCTGGAACGCCCGCTTTAAGGTGGGTCCGGTACCGATCTTTTATAGCC CCTATTTGCAGTTGCCGGTGGGTGACAAACGTCGCTCTGGTTTCTTGATCCCGAACGCCAAGTACACCACCACCAACTAC TTTGAGTTCTACCTGCCATATTACTGGAACATCGCGCCAAATATGGATGCCACCATCACGCCGCATTATATGCATCGTCG TGGCAACATCATGTGGGAGAACGAATTCCGCTACCTCTCCCAGGCGGGCGCTGGCTTGATGGAACTGGACTATCTGCCTT CAGATAAAGTCTATGAAGATGAACACCCGAACGATGACAGTTCACGTCGTTGGTTATTCTACTGGAACCACTCCGGGGTC ATGGATCAGGTGTGGCGTTTCAACGTCGACTACACCAAGGTCAGCGATCCTAGCTACTTCAATGATTTCGATAACAAGTA CGGTTCCAGTACTGACGGCTACGCAACGCAAAAATTCAGCGTTGGCTATGCGGTGCAAAACTTCAATGCCACCGTTTCAA CCAAGCAGTTCCAGGTTTTCAGCGAACAGAACACCAGTAGCTACTCGGCAGAGCCGCAGTTAGACGTTAATTACTACCAG AATGATGTTGGTCCGTTTGATACGCGTATTTACGGCCAGGCAGTGCACTTTGTTAACACCAGAGACGACATGCCTGAAGC AACCCGTGTTCACCTGGAACCGACCATCAATTTGCCGCTCTCTAATAACTGGGGCAGCATCAATACCGAAGCGAAGTTTC TGGCAACCCATTATCAGCAAACCAATCTTGACTGGTATAACTCCAGAAACACGACCAAGCTGGACGAATCCGTTAACCGC GTAATGCCGCAATTCAAAGTTGACGGCAAAATGGTCTTTGAACGCGATATGGAAATGCTGGCTCCGGGTTATACCCAAAC GCTGGAACCGCGCGCGCAGTATTTGTACGTGCCGTATCGCGATCAGAGCGACATCTATAACTACGACTCGTCTCTGCTGC AATCTGACTACTCTGGCCTGTTCCGGGACCGGACTTACGGCGGTCTTGACCGTATTGCCTCCGCTAACCAAGTGACGACC GGTGTCACATCTCGCATATATGATGATGCTGCCGTTGAACGTTTTAATATTTCCGTTGGTCAAATCTACTATTTCACGGA GTCTCGCACTGGCGATGACAACATAACATGGGAGAATGACGACAAAACGGGCTCACTGGTGTGGGCAGGCGACACCTACT GGCGTATCTCCGAGCGTTGGGGATTGCGTGGCGGGATTCAGTACGATACACGTCTGGATAACGTAGCGACCAGTAACTCC AGCATTGAATACCGTCGGGATGAAGACCGTCTGGTACAGCTGAATTACCATTACGCCAGCCCGGAATATATTCAGGCTAC GCTGCCTAAGTACTATTCCACTGCTGAGCAATATAAGAATGGTATTTCGCAGGTAGGTGCTGTCGCCAGCAGGCCAATTG CCGATCGTTGGTCCATTGTTGGGGCCTACTACTACGACACCAATGCGAACAAGCAAGCCGACTCTATGTTAGGTGTGCAA TACAGCTCCTGCTGCTATGCAATTCGCGTCGGTTACGAGCGGAAGCTGAACGGTTGGGATAACGATAAACAACATGCGGT ATATGACAACGCAATCGGCTTTAACATCGAACTTCGCGGCCTGAGCTCCAACTACGGTCTGGGTACGCAAGAGATGCTGC GTTCGAACATTCTGCCGTATCAAAACACTTTGTGA
Upstream 100 bases:
>100_bases TTCGGACAGGATTAACACTAGCGTAGAGATGACGAGTACGTTAGTCTCTGACCGTTTGTCACGCGCAACGTTACCGATGA TGGAACAATAAAATCAACGT
Downstream 100 bases:
>100_bases TTTGTTGATTTACCACGTAATCCGCAGTGCGGTTAATTGAAATGGAAAAAGTATGAAGAACTGGAAAACGCTGCTTCTCG GTATCGCCATGATCGCGAAT
Product: organic solvent tolerance protein
Products: NA
Alternate protein names: Organic solvent tolerance protein [H]
Number of amino acids: Translated: 784; Mature: 784
Protein sequence:
>784_residues MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKFLATHYQQTNLDWYNSRNTTKLDESVNR VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS SIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGISQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQ YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
Sequences:
>Translated_784_residues MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKFLATHYQQTNLDWYNSRNTTKLDESVNR VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS SIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGISQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQ YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL >Mature_784_residues MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNS RLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGV MDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQ NDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTEAKFLATHYQQTNLDWYNSRNTTKLDESVNR VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTT GVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNS SIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGISQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQ YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL
Specific function: Together with lptE, is involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]
COG id: COG1452
COG function: function code M; Organic solvent tolerance protein OstA
Gene ontology:
Cell location: Cell outer membrane [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lptD family [H]
Homologues:
Organism=Escherichia coli, GI1786239, Length=784, Percent_Identity=99.6173469387755, Blast_Score=1620, Evalue=0.0,
Paralogues:
None
Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR020889 - InterPro: IPR005653 - InterPro: IPR007543 [H]
Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]
EC number: NA
Molecular weight: Translated: 89657; Mature: 89657
Theoretical pI: Translated: 4.76; Mature: 4.76
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAK CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEECCCCCCCCEEEECCCCC GDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILK CCCCCCCEEECEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEE GPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTW CCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH SVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY HHHHHHHHHCHHHHHHHHHCCCEEECCEEEEECCEEECCCCCCCCCCEECCCCCEEECCE FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYED EEEEEEEEEEECCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCC EHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFS CCCCCCCCCEEEEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEE VGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNT EEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC RDDMPEATRVHLEPTINLPLSNNWGSINTEAKFLATHYQQTNLDWYNSRNTTKLDESVNR CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHH VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGL HCCCEECCCEEEEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH FRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWEND HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCC DKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYAS CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECC PEYIQATLPKYYSTAEQYKNGISQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHHCCC YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPY HHCEEEEEEECCEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCC QNTL CCCC >Mature Secondary Structure MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQGDTNDLPVTINADHAK CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEECCCCCCCCEEEECCCCC GDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVRTVDALGNVHYDDNQVILK CCCCCCCEEECEEEEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHCCCEECCCEEEEE GPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGENRYTILDNGSFTSCLPGSDTW CCCCCCCCCCCCCEEECCHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCCCCCCHH SVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQLPVGDKRRSGFLIPNAKYTTTNY HHHHHHHHHCHHHHHHHHHCCCEEECCEEEEECCEEECCCCCCCCCCEECCCCCEEECCE FEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEFRYLSQAGAGLMELDYLPSDKVYED EEEEEEEEEEECCCCCCEECHHHHHHCCCEEEEHHHHHHHHCCCCEEEEECCCCCCCCCC EHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKVSDPSYFNDFDNKYGSSTDGYATQKFS CCCCCCCCCEEEEEECCCCHHHHHHEECCCEEECCCCCHHCHHHHHCCCCCCCCEEEEEE VGYAVQNFNATVSTKQFQVFSEQNTSSYSAEPQLDVNYYQNDVGPFDTRIYGQAVHFVNT EEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEEECC RDDMPEATRVHLEPTINLPLSNNWGSINTEAKFLATHYQQTNLDWYNSRNTTKLDESVNR CCCCCCCEEEEEEEEEECCCCCCCCCCCCCCEEEEEHHHHCCCCEECCCCCCHHHHHHHH VMPQFKVDGKMVFERDMEMLAPGYTQTLEPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGL HCCCEECCCEEEEHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH FRDRTYGGLDRIASANQVTTGVTSRIYDDAAVERFNISVGQIYYFTESRTGDDNITWEND HHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEEECEEEEEEEEECCCCCCCCCEECCC DKTGSLVWAGDTYWRISERWGLRGGIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYAS CCCCCEEECCCCEEEEECCCCCCCCCEECCCCCCEECCCCCEEEECCCCEEEEEEEEECC PEYIQATLPKYYSTAEQYKNGISQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQ CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHHCCC YSSCCYAIRVGYERKLNGWDNDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPY HHCEEEEEEECCEEECCCCCCCCCCEEECCCEEEEEEEEECCCCCCCCHHHHHHHCCCCC QNTL CCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA