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The map label for this gene is papL

Identifier: 30024049

GI number: 30024049

Start: 1602114

End: 1603310

Strand: Reverse

Name: papL

Synonym: L0324

Alternate gene names: 30024049

Gene position: 1603310-1602114 (Counterclockwise)

Preceding gene: 15673542

Following gene: 15673540

Centisome position: 67.78

GC content: 36.17

Gene sequence:

>1197_bases
ATGAAATTAGAAAAATTACCCGAGGAGTTTGTACAGGCCAAGCCGGTTTTAGAAAAAATAATCGAGCATGGGTTTGAAGC
TTATTTTGTGGGAGGTTCTGTACGTGATATATTGCTTGGGCGAGAGATTCACGATGTGGATATTGCAACAAGTGCATATC
CAGAGGAAATTAAAGATATTTTTCCTTATACCATTGATGTGGGAATCGAACATGGAACAGTACTTGTTTTAGCTGGAAAA
TCAGAAGCTGAGCATTATGAAATTACTACTTTTCGGACAGAATCAAAATATACAGATTACAGACGGCCAGATCATGTGGA
TTTCGTCAGAGATTTACGAGAGGATTTAAAACGTCGTGACTTCACGATCAATGCCTTTGCTTGTGATGTTAATGGGCAAA
TTATTGATTTGTTTGATGGACTGACGGATTTGAAGGAACGCAGGCTAACAGCGGTTGGTAGCGCTTTGGAACGTTTTAAT
GAAGATGCTTTACGGATTATGCGGGCCATGCGATTCGCAGCAACTTTAGACTTTCAAATTGAAAATAAAACTTTCTTAGC
CATGTGTGAGTCGGCTCACTTACTGGAAAAAATCTCAGTTGAACGAATTTTTATTGAATTTGATAAGCTGTTGTTAGGTC
AGGATTGGCGAAATGGTTTGACCTTACTGCTTAAATCAGGGGCTTATAAATATTTACCAGATTTGCAAGATTCGGCCTTG
AAAAAAGTACTGACAGACTTGTCAGTAGATTTCCATTTTCAGAATTCTGAGCAAGCATGGGCAGCTTTACTGACCAGATT
TTCCAATGTTGATGTGAAAACATTTTTGCGAAAATGGAAAGTGTCCAATGAATTTGCAAAATTTGTAGCAGACCTTGTAT
CAGCTTATGAACTTGACTCTTGGGATTTGATGAGTCTTTATCATTTTGGTTTAGAAAAAGTGCTTTTGGTTGATGAATTG
AAAGTAGCTTATGGTTTGAAGATTAATAGAGAACAAGCTGTCACTATTAATAACCAACTTCAAATTCATGATAAATCAGA
GATTGTCATTGCTGGAAAAGATTTAATGGAGGAATTTTCGCTTGAACCTGGTCCAGAACTTGGTAAAATTTTAAAAATTA
TTGAAGAAAAAATTGTCAAAAATGAATTGAAAAATGAACAGGCTGCTATTTTTGCAGAAGTTAAAAAAATGCTCTGA

Upstream 100 bases:

>100_bases
AAGTGCTGACAGGTCTGTATAAACTTTCTGTCAGTAAAATTTTAAGGTGTACAATGGGGCTATGCCCCGTTTTTGTTTAA
AAAATGATAAAATAATAGAT

Downstream 100 bases:

>100_bases
TTAAATTTTACTGACAGAAATTTTGGCAAATAACCAATTGAAATTTAGTATTTATAATAAATACAATTTCTTGGTTTCTA
AGTAATGTATTTTATAGATT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKLEKLPEEFVQAKPVLEKIIEHGFEAYFVGGSVRDILLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTINAFACDVNGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFAATLDFQIENKTFLAMCESAHLLEKISVERIFIEFDKLLLGQDWRNGLTLLLKSGAYKYLPDLQDSAL
KKVLTDLSVDFHFQNSEQAWAALLTRFSNVDVKTFLRKWKVSNEFAKFVADLVSAYELDSWDLMSLYHFGLEKVLLVDEL
KVAYGLKINREQAVTINNQLQIHDKSEIVIAGKDLMEEFSLEPGPELGKILKIIEEKIVKNELKNEQAAIFAEVKKML

Sequences:

>Translated_398_residues
MKLEKLPEEFVQAKPVLEKIIEHGFEAYFVGGSVRDILLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTINAFACDVNGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFAATLDFQIENKTFLAMCESAHLLEKISVERIFIEFDKLLLGQDWRNGLTLLLKSGAYKYLPDLQDSAL
KKVLTDLSVDFHFQNSEQAWAALLTRFSNVDVKTFLRKWKVSNEFAKFVADLVSAYELDSWDLMSLYHFGLEKVLLVDEL
KVAYGLKINREQAVTINNQLQIHDKSEIVIAGKDLMEEFSLEPGPELGKILKIIEEKIVKNELKNEQAAIFAEVKKML
>Mature_398_residues
MKLEKLPEEFVQAKPVLEKIIEHGFEAYFVGGSVRDILLGREIHDVDIATSAYPEEIKDIFPYTIDVGIEHGTVLVLAGK
SEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRDFTINAFACDVNGQIIDLFDGLTDLKERRLTAVGSALERFN
EDALRIMRAMRFAATLDFQIENKTFLAMCESAHLLEKISVERIFIEFDKLLLGQDWRNGLTLLLKSGAYKYLPDLQDSAL
KKVLTDLSVDFHFQNSEQAWAALLTRFSNVDVKTFLRKWKVSNEFAKFVADLVSAYELDSWDLMSLYHFGLEKVLLVDEL
KVAYGLKINREQAVTINNQLQIHDKSEIVIAGKDLMEEFSLEPGPELGKILKIIEEKIVKNELKNEQAAIFAEVKKML

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=208, Percent_Identity=35.5769230769231, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI87081691, Length=207, Percent_Identity=35.2657004830918, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=36.2385321100917, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71995920, Length=200, Percent_Identity=35, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321016, Length=208, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281360112, Length=201, Percent_Identity=36.81592039801, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21357337, Length=201, Percent_Identity=36.81592039801, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_LACLA (Q9CFC2)

Other databases:

- EMBL:   AE005176
- PIR:   G86819
- RefSeq:   NP_267715.2
- ProteinModelPortal:   Q9CFC2
- SMR:   Q9CFC2
- GeneID:   1115219
- GenomeReviews:   AE005176_GR
- KEGG:   lla:L0324
- NMPDR:   fig|272623.1.peg.1601
- HOGENOM:   HBG635063
- OMA:   DHGNGYE
- ProtClustDB:   PRK13299
- BioCyc:   LLAC272623:L0324-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01263
- InterPro:   IPR023068
- InterPro:   IPR002646

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45781; Mature: 45781

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 35-35 BINDING 119-119 BINDING 162-162 BINDING 165-165 BINDING 168-168 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEKLPEEFVQAKPVLEKIIEHGFEAYFVGGSVRDILLGREIHDVDIATSAYPEEIKDI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FPYTIDVGIEHGTVLVLAGKSEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRD
CCEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
FTINAFACDVNGQIIDLFDGLTDLKERRLTAVGSALERFNEDALRIMRAMRFAATLDFQI
CEEEEEEEECCCCEEHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEE
ENKTFLAMCESAHLLEKISVERIFIEFDKLLLGQDWRNGLTLLLKSGAYKYLPDLQDSAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
KKVLTDLSVDFHFQNSEQAWAALLTRFSNVDVKTFLRKWKVSNEFAKFVADLVSAYELDS
HHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WDLMSLYHFGLEKVLLVDELKVAYGLKINREQAVTINNQLQIHDKSEIVIAGKDLMEEFS
HHHHHHHHHCHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEECCCCEEEECHHHHHHCC
LEPGPELGKILKIIEEKIVKNELKNEQAAIFAEVKKML
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
>Mature Secondary Structure
MKLEKLPEEFVQAKPVLEKIIEHGFEAYFVGGSVRDILLGREIHDVDIATSAYPEEIKDI
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCHHHHHHH
FPYTIDVGIEHGTVLVLAGKSEAEHYEITTFRTESKYTDYRRPDHVDFVRDLREDLKRRD
CCEEEEEECCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
FTINAFACDVNGQIIDLFDGLTDLKERRLTAVGSALERFNEDALRIMRAMRFAATLDFQI
CEEEEEEEECCCCEEHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEE
ENKTFLAMCESAHLLEKISVERIFIEFDKLLLGQDWRNGLTLLLKSGAYKYLPDLQDSAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
KKVLTDLSVDFHFQNSEQAWAALLTRFSNVDVKTFLRKWKVSNEFAKFVADLVSAYELDS
HHHHHHCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
WDLMSLYHFGLEKVLLVDELKVAYGLKINREQAVTINNQLQIHDKSEIVIAGKDLMEEFS
HHHHHHHHHCHHHHHHHHHHHHHHCEEECCCCEEEECCEEEEECCCCEEEECHHHHHHCC
LEPGPELGKILKIIEEKIVKNELKNEQAAIFAEVKKML
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471