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The map label for this gene is insF1 [C]

Identifier: 29349698

GI number: 29349698

Start: 5651292

End: 5652011

Strand: Reverse

Name: insF1 [C]

Synonym: BT_4290

Alternate gene names: 29349698

Gene position: 5652011-5651292 (Counterclockwise)

Preceding gene: 29349699

Following gene: 29349695

Centisome position: 90.28

GC content: 44.44

Gene sequence:

>720_bases
ATGCCCAAAGCTGGTATGCGCGAGTTATACGCCTGCTGCGTGAGTAAGTTTGGACCAAAGATGGTTATCGGTCGTGATCG
TTGTTATGACATTTTTCGCTCCAATGGTTTGTGTCAACGTACAAGTCGCAAGCGTCCTAAAACAACGAATTCGAACCATA
ATTACTATATCTACCCCGATCTGTTGAATGTTGCACCCAAATTTGTCGCTACAAGATTGGGCGCTATGGTAGTGGCGGAT
ATAACCTACGTAAACACCGGTCAGGGCTGGGCTTACCTCTCGTTGCTTACCGATGCGTCAAGTCGTGCCATCGTGGGATA
TGCGCTTTACAAAACTCTTGAGACGGAGGGGCCCTTGAAAGCTTTGGAGATGGCAATATCATTCTATGAGAAGTATCACA
TAGACATGAGCACTCTTATTCATCATTCCGACCGGGGTGTCCAATATTGCTCAAATAAATATGTAGAGAGGCTTAAAGAG
CATCAAATCAACATCAGTATGACGCAGTGTGGTGATCCTTTACATAATGCATTGGCTGAAAGAATGAACAACACCATTAA
AAACGGTTGGCTATTCGACTGTGATGATGAGAGCTTCGAGCAAGTAAGCAAGCGCATTGAAGATGCTGTATATGTATATA
ATCATGTGCGGCCTCATCAAGGGATAAACATGAGGACACCTATGGAAGTGGTCAGCGAAACGGGAGGATTAACAGCATAA

Upstream 100 bases:

>100_bases
CATAACCCGACAAGGTTATTACAAGCATGTGAACCGACATTTGGAGGTTGATATCCTTACCACAAGCATCGTGTTGTACT
GCAAAGAACTGTTGGAACTC

Downstream 100 bases:

>100_bases
TACCCGCTCGTCGGGACGGGTGGTCCCGCCCCTTGCCGCTAGGGCGATCCCCGACCGAAGAGTTTTTGGTTGTTGGCAAC
ATTACATTTTCCTGAAGAAG

Product: transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MPKAGMRELYACCVSKFGPKMVIGRDRCYDIFRSNGLCQRTSRKRPKTTNSNHNYYIYPDLLNVAPKFVATRLGAMVVAD
ITYVNTGQGWAYLSLLTDASSRAIVGYALYKTLETEGPLKALEMAISFYEKYHIDMSTLIHHSDRGVQYCSNKYVERLKE
HQINISMTQCGDPLHNALAERMNNTIKNGWLFDCDDESFEQVSKRIEDAVYVYNHVRPHQGINMRTPMEVVSETGGLTA

Sequences:

>Translated_239_residues
MPKAGMRELYACCVSKFGPKMVIGRDRCYDIFRSNGLCQRTSRKRPKTTNSNHNYYIYPDLLNVAPKFVATRLGAMVVAD
ITYVNTGQGWAYLSLLTDASSRAIVGYALYKTLETEGPLKALEMAISFYEKYHIDMSTLIHHSDRGVQYCSNKYVERLKE
HQINISMTQCGDPLHNALAERMNNTIKNGWLFDCDDESFEQVSKRIEDAVYVYNHVRPHQGINMRTPMEVVSETGGLTA
>Mature_238_residues
PKAGMRELYACCVSKFGPKMVIGRDRCYDIFRSNGLCQRTSRKRPKTTNSNHNYYIYPDLLNVAPKFVATRLGAMVVADI
TYVNTGQGWAYLSLLTDASSRAIVGYALYKTLETEGPLKALEMAISFYEKYHIDMSTLIHHSDRGVQYCSNKYVERLKEH
QINISMTQCGDPLHNALAERMNNTIKNGWLFDCDDESFEQVSKRIEDAVYVYNHVRPHQGINMRTPMEVVSETGGLTA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=150, Percent_Identity=37.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788405, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787263, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786753, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786570, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786490, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 27199; Mature: 27068

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKAGMRELYACCVSKFGPKMVIGRDRCYDIFRSNGLCQRTSRKRPKTTNSNHNYYIYPD
CCCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCC
LLNVAPKFVATRLGAMVVADITYVNTGQGWAYLSLLTDASSRAIVGYALYKTLETEGPLK
HHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHH
ALEMAISFYEKYHIDMSTLIHHSDRGVQYCSNKYVERLKEHQINISMTQCGDPLHNALAE
HHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHH
RMNNTIKNGWLFDCDDESFEQVSKRIEDAVYVYNHVRPHQGINMRTPMEVVSETGGLTA
HHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCC
>Mature Secondary Structure 
PKAGMRELYACCVSKFGPKMVIGRDRCYDIFRSNGLCQRTSRKRPKTTNSNHNYYIYPD
CCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCC
LLNVAPKFVATRLGAMVVADITYVNTGQGWAYLSLLTDASSRAIVGYALYKTLETEGPLK
HHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCHHH
ALEMAISFYEKYHIDMSTLIHHSDRGVQYCSNKYVERLKEHQINISMTQCGDPLHNALAE
HHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEHHHCCCHHHHHHHH
RMNNTIKNGWLFDCDDESFEQVSKRIEDAVYVYNHVRPHQGINMRTPMEVVSETGGLTA
HHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]