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The map label for this gene is potD [H]

Identifier: 29346698

GI number: 29346698

Start: 1604650

End: 1605999

Strand: Reverse

Name: potD [H]

Synonym: BT_1288

Alternate gene names: 29346698

Gene position: 1605999-1604650 (Counterclockwise)

Preceding gene: 29346699

Following gene: 29346679

Centisome position: 25.65

GC content: 44.67

Gene sequence:

>1350_bases
ATGAAAAGAATAGTTGCTGTTAACTTGTTTTTGCTGGTGCTGGCTGGTTTTCTGAGCGGTTGCTACAATTCCGGCGAGCC
ACGCGAGAGAGTTCTTAAGATCTATAACTGGGCAGATTATATAGGAGAAGGTGTACTCGAAGATTTCCAGGCGTACTATA
AAGAACAGACAGGTGAAGATATCCGCATCGTCTATCAGACATTCGACATCAACGAGATCATGTTGACGAAAATAGAAAAA
GGACATGAGGATTTTGATGTGGTTTGTCCTTCGGAATATATCATTGAGAGAATGCTGAAAAAGCATCTGTTGTTGCCTAT
TGACACCACTTTCGCCCATTCCCCTAATTACATGAATAATGTGTCACCATTTATTCGCGAACAGATTAATAAACTGAGTC
AGCCGGGTGAAGAAGCAAGCCGTTACGCTGTCTGCTATATGTGGGGGACCGCAGGTATTCTTTATAACCGGGCATACGTT
CCCGATTCCGACGCTTTCTCCTGGGAATGTCTTTGGGATAAGAAATATGCAGGAAAGATTCTGATGAAAGACAGTTACCG
TGACGCTTATGGAACAGCTGTTATCTATGCCCATGCCAAAGAGCTGGAAGAAGGGACGGTGACGGTGGAAGACCTGATGA
ATGATTACTCGCCACGTGCTATGGAGGTAGCCGAAAAGTATCTGAAAGCTATGAAACCGAATATTGCCGGTTGGGAAGCG
GACTTCGGTAAGGAGATGATGACGAAGAATAAAGCATGGCTGAATATGACCTGGAGTGGCGATGCGATATGGGCGATTGA
AGAGGCGAATGCGGTAGGAGTGGACCTTGACTACGTGGTTCCGAAAGAAGGAAGTAATATCTGGTATGATGGCTGGGTGA
TTCCTAAATATGCGAAGAATCCGGTGGCTGCCAGCTACTTTATCAACTTTATGTGCCGTCCGGACATTGCGTTGCGTAAT
ATGGATTTCTGTGGATATGTGAGTTCTATCGCTACACCGGAGATTCTGGAAGAGAAAGTGGATACGACTCTGGATTATTA
TGCGGATTTAAGTTATTTCTTCGATCCGGATGCCGACAGCATACAGATTGATAAGATTCAATATCCCGACCGTAAGGTGG
TGGAACGCTGCGCTATGATTCGTGACTTCGGAGATAAAACCAAAGAGGTACTGGATATCTGGTCACGCATCAAAGGTGAC
AACCTGGGGGTAGGTATTACTATTCTGATTTTTGTTGTCGTAGCTTTGATGAGCGGATGGATGATTTATAAGAGATGGCA
ACGCTACAGCCGTCGGAAACAACAAAATCGCAGAAGCAGAAGAAAGAGAAAAAAGAATGTGAAGCGGTAA

Upstream 100 bases:

>100_bases
TTATTTTTGTGGTGGTACTGGCTTTACTGATAGTAATTAATTACCGTGCCGGAAAAACGAAGAAGAAAGAATCATGAACA
TGTACTGCTACGTAAATAAA

Downstream 100 bases:

>100_bases
GTTAAAAACACTCGGTAATAAGCGGTGGATGATAAACGAATGATACGGTTAAAACCGGGCATCGTTTGTCATCCACCGTT
TTTTATCCTACTCTTTTCAT

Product: spermidine/putrescine ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MKRIVAVNLFLLVLAGFLSGCYNSGEPRERVLKIYNWADYIGEGVLEDFQAYYKEQTGEDIRIVYQTFDINEIMLTKIEK
GHEDFDVVCPSEYIIERMLKKHLLLPIDTTFAHSPNYMNNVSPFIREQINKLSQPGEEASRYAVCYMWGTAGILYNRAYV
PDSDAFSWECLWDKKYAGKILMKDSYRDAYGTAVIYAHAKELEEGTVTVEDLMNDYSPRAMEVAEKYLKAMKPNIAGWEA
DFGKEMMTKNKAWLNMTWSGDAIWAIEEANAVGVDLDYVVPKEGSNIWYDGWVIPKYAKNPVAASYFINFMCRPDIALRN
MDFCGYVSSIATPEILEEKVDTTLDYYADLSYFFDPDADSIQIDKIQYPDRKVVERCAMIRDFGDKTKEVLDIWSRIKGD
NLGVGITILIFVVVALMSGWMIYKRWQRYSRRKQQNRRSRRKRKKNVKR

Sequences:

>Translated_449_residues
MKRIVAVNLFLLVLAGFLSGCYNSGEPRERVLKIYNWADYIGEGVLEDFQAYYKEQTGEDIRIVYQTFDINEIMLTKIEK
GHEDFDVVCPSEYIIERMLKKHLLLPIDTTFAHSPNYMNNVSPFIREQINKLSQPGEEASRYAVCYMWGTAGILYNRAYV
PDSDAFSWECLWDKKYAGKILMKDSYRDAYGTAVIYAHAKELEEGTVTVEDLMNDYSPRAMEVAEKYLKAMKPNIAGWEA
DFGKEMMTKNKAWLNMTWSGDAIWAIEEANAVGVDLDYVVPKEGSNIWYDGWVIPKYAKNPVAASYFINFMCRPDIALRN
MDFCGYVSSIATPEILEEKVDTTLDYYADLSYFFDPDADSIQIDKIQYPDRKVVERCAMIRDFGDKTKEVLDIWSRIKGD
NLGVGITILIFVVVALMSGWMIYKRWQRYSRRKQQNRRSRRKRKKNVKR
>Mature_449_residues
MKRIVAVNLFLLVLAGFLSGCYNSGEPRERVLKIYNWADYIGEGVLEDFQAYYKEQTGEDIRIVYQTFDINEIMLTKIEK
GHEDFDVVCPSEYIIERMLKKHLLLPIDTTFAHSPNYMNNVSPFIREQINKLSQPGEEASRYAVCYMWGTAGILYNRAYV
PDSDAFSWECLWDKKYAGKILMKDSYRDAYGTAVIYAHAKELEEGTVTVEDLMNDYSPRAMEVAEKYLKAMKPNIAGWEA
DFGKEMMTKNKAWLNMTWSGDAIWAIEEANAVGVDLDYVVPKEGSNIWYDGWVIPKYAKNPVAASYFINFMCRPDIALRN
MDFCGYVSSIATPEILEEKVDTTLDYYADLSYFFDPDADSIQIDKIQYPDRKVVERCAMIRDFGDKTKEVLDIWSRIKGD
NLGVGITILIFVVVALMSGWMIYKRWQRYSRRKQQNRRSRRKRKKNVKR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=372, Percent_Identity=27.9569892473118, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1787078, Length=341, Percent_Identity=25.8064516129032, Blast_Score=112, Evalue=7e-26,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 52169; Mature: 52169

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIVAVNLFLLVLAGFLSGCYNSGEPRERVLKIYNWADYIGEGVLEDFQAYYKEQTGED
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IRIVYQTFDINEIMLTKIEKGHEDFDVVCPSEYIIERMLKKHLLLPIDTTFAHSPNYMNN
EEEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VSPFIREQINKLSQPGEEASRYAVCYMWGTAGILYNRAYVPDSDAFSWECLWDKKYAGKI
CCHHHHHHHHHHCCCCCHHHCEEEEEEECCCHHHCCCCCCCCCCCCEEEEEECCCCCCEE
LMKDSYRDAYGTAVIYAHAKELEEGTVTVEDLMNDYSPRAMEVAEKYLKAMKPNIAGWEA
EEECCCCHHHCCEEEEEEHHHHCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
DFGKEMMTKNKAWLNMTWSGDAIWAIEEANAVGVDLDYVVPKEGSNIWYDGWVIPKYAKN
HHHHHHHHCCCCEEEEEECCCEEEEEECCCEEECEEEEECCCCCCCEEECCEECCCCCCC
PVAASYFINFMCRPDIALRNMDFCGYVSSIATPEILEEKVDTTLDYYADLSYFFDPDADS
CCHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCC
IQIDKIQYPDRKVVERCAMIRDFGDKTKEVLDIWSRIKGDNLGVGITILIFVVVALMSGW
EEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCH
MIYKRWQRYSRRKQQNRRSRRKRKKNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRIVAVNLFLLVLAGFLSGCYNSGEPRERVLKIYNWADYIGEGVLEDFQAYYKEQTGED
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IRIVYQTFDINEIMLTKIEKGHEDFDVVCPSEYIIERMLKKHLLLPIDTTFAHSPNYMNN
EEEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VSPFIREQINKLSQPGEEASRYAVCYMWGTAGILYNRAYVPDSDAFSWECLWDKKYAGKI
CCHHHHHHHHHHCCCCCHHHCEEEEEEECCCHHHCCCCCCCCCCCCEEEEEECCCCCCEE
LMKDSYRDAYGTAVIYAHAKELEEGTVTVEDLMNDYSPRAMEVAEKYLKAMKPNIAGWEA
EEECCCCHHHCCEEEEEEHHHHCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
DFGKEMMTKNKAWLNMTWSGDAIWAIEEANAVGVDLDYVVPKEGSNIWYDGWVIPKYAKN
HHHHHHHHCCCCEEEEEECCCEEEEEECCCEEECEEEEECCCCCCCEEECCEECCCCCCC
PVAASYFINFMCRPDIALRNMDFCGYVSSIATPEILEEKVDTTLDYYADLSYFFDPDADS
CCHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCCCCC
IQIDKIQYPDRKVVERCAMIRDFGDKTKEVLDIWSRIKGDNLGVGITILIFVVVALMSGW
EEEEEEECCCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCH
MIYKRWQRYSRRKQQNRRSRRKRKKNVKR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]