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The map label for this gene is hpaIIR [H]

Identifier: 29346559

GI number: 29346559

Start: 1433635

End: 1434720

Strand: Reverse

Name: hpaIIR [H]

Synonym: BT_1149

Alternate gene names: 29346559

Gene position: 1434720-1433635 (Counterclockwise)

Preceding gene: 29346560

Following gene: 29346558

Centisome position: 22.92

GC content: 45.12

Gene sequence:

>1086_bases
ATGGCATTTGAAGCAACAAAAAGAGAATGGTGTGAACTGTATACTTTCTTCCGTCTGCTGACAGAAGGAAAAGTAACGTT
GGGTACGGCTAAGGCAAAGAAAGGAGAAATCGACTGGCCTATTGCAATGATTCAACGGGAGGAACATGACGGAACACGCT
GCTACTATATAGAAAAAGAGATGGTCCGCATCAAAGGAGAAAATAGTGAGAAATTCGTTCCCCGGGAAGATTTTGGGATT
GTGGCAGATTTGATTTTGCAGGCTGTCAAGTCCTCTTCGGAGGATGAAGTCACTTCTCCGGATGGAGTGGAAGAGTTTTT
GGATGAAGTCGCAATTTTCGATCTGGAAGCGAAGACGGAAGATCGTACCGATTTTTATATTGCCTTCTGGCATCCGGAGG
CACCGTTGAGCGGGTTCAGCGTTCGTTCGCGATTGGGTGCTATGAATCCGCTGTTGGACGGAGGCCGTGCGGCTAACTTG
AAACTGGAACAGAGCGGAGTCAAATTTGCAACACCTACGGTGAATAAGATTAATGCGTTGCCGGAAGCCCCTAATGAAGT
AGCAGAACGAATGTTGCTGATTGAGCGCTTGGGTGGTGTACTCAAATACTCGGACGTGGCAGATCGGGTATTCCGTAGCA
ATCTTTTAATGATTGACTTACACTTTCCTCGTGTGCTGACAGAAATGGTGCGCATCATGCACTTGGACGACATCACCCGT
ATCAGTGAACTGACGGAAGTAATCAAACAGATGAACCCGTTGAAAATAAAAGATGAATTGGTAAACAAACATGGTTTCTA
TGAGTTTAAAGTGAAGCAATTCCTGATGGCATTAGCCTTGGGTATGCGTCCGGCAAAGATATATACTGGACAGGATTCGG
CTGTTGAAGGTATCCTGTTGATGGATGGGAGTGGGGAAGTTCTTTGCTATCACAAGTCAGAGAAACCGGTTATGGAGGAC
TTCCTGTTCTTGAATACCCGTTTGGAGAAAGGTTCTCTGGATAAGGACAAATACGGCTTTTTGGAAAGAGAGAACGGTAC
GTATTACTTCAAACTGAATGCAAAGATCGGATTGGTAAAACGATAA

Upstream 100 bases:

>100_bases
TATTCGTCGTTCCATGATGCCCAAACTGCGTGCACGATTGGAGAAAGTGGAGACCGCAAGGATTCTTGATGAAGAGTAAA
ATAAAGAAAAATAAGAATAT

Downstream 100 bases:

>100_bases
ATGCAAGTGGTGAACGAGTTGTAAGTATAGTAAATATAGTATATGAAAACGAATGAAGTTTTAGAAAATATCAAAGCACG
TCGTAGTGTACGGGCTTATA

Product: typeII restriction enzyme HpaII

Products: NA

Alternate protein names: R.HpaII; Endonuclease HpaII; Type II restriction enzyme HpaII [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAFEATKREWCELYTFFRLLTEGKVTLGTAKAKKGEIDWPIAMIQREEHDGTRCYYIEKEMVRIKGENSEKFVPREDFGI
VADLILQAVKSSSEDEVTSPDGVEEFLDEVAIFDLEAKTEDRTDFYIAFWHPEAPLSGFSVRSRLGAMNPLLDGGRAANL
KLEQSGVKFATPTVNKINALPEAPNEVAERMLLIERLGGVLKYSDVADRVFRSNLLMIDLHFPRVLTEMVRIMHLDDITR
ISELTEVIKQMNPLKIKDELVNKHGFYEFKVKQFLMALALGMRPAKIYTGQDSAVEGILLMDGSGEVLCYHKSEKPVMED
FLFLNTRLEKGSLDKDKYGFLERENGTYYFKLNAKIGLVKR

Sequences:

>Translated_361_residues
MAFEATKREWCELYTFFRLLTEGKVTLGTAKAKKGEIDWPIAMIQREEHDGTRCYYIEKEMVRIKGENSEKFVPREDFGI
VADLILQAVKSSSEDEVTSPDGVEEFLDEVAIFDLEAKTEDRTDFYIAFWHPEAPLSGFSVRSRLGAMNPLLDGGRAANL
KLEQSGVKFATPTVNKINALPEAPNEVAERMLLIERLGGVLKYSDVADRVFRSNLLMIDLHFPRVLTEMVRIMHLDDITR
ISELTEVIKQMNPLKIKDELVNKHGFYEFKVKQFLMALALGMRPAKIYTGQDSAVEGILLMDGSGEVLCYHKSEKPVMED
FLFLNTRLEKGSLDKDKYGFLERENGTYYFKLNAKIGLVKR
>Mature_360_residues
AFEATKREWCELYTFFRLLTEGKVTLGTAKAKKGEIDWPIAMIQREEHDGTRCYYIEKEMVRIKGENSEKFVPREDFGIV
ADLILQAVKSSSEDEVTSPDGVEEFLDEVAIFDLEAKTEDRTDFYIAFWHPEAPLSGFSVRSRLGAMNPLLDGGRAANLK
LEQSGVKFATPTVNKINALPEAPNEVAERMLLIERLGGVLKYSDVADRVFRSNLLMIDLHFPRVLTEMVRIMHLDDITRI
SELTEVIKQMNPLKIKDELVNKHGFYEFKVKQFLMALALGMRPAKIYTGQDSAVEGILLMDGSGEVLCYHKSEKPVMEDF
LFLNTRLEKGSLDKDKYGFLERENGTYYFKLNAKIGLVKR

Specific function: Recognizes the double-stranded sequence CCGG and cleaves after C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019062 [H]

Pfam domain/function: PF09561 RE_HpaII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 41216; Mature: 41085

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEATKREWCELYTFFRLLTEGKVTLGTAKAKKGEIDWPIAMIQREEHDGTRCYYIEKE
CCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEEEE
MVRIKGENSEKFVPREDFGIVADLILQAVKSSSEDEVTSPDGVEEFLDEVAIFDLEAKTE
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEECCCC
DRTDFYIAFWHPEAPLSGFSVRSRLGAMNPLLDGGRAANLKLEQSGVKFATPTVNKINAL
CCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCEECEEEECCCCEEECCCHHHHHCC
PEAPNEVAERMLLIERLGGVLKYSDVADRVFRSNLLMIDLHFPRVLTEMVRIMHLDDITR
CCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHH
ISELTEVIKQMNPLKIKDELVNKHGFYEFKVKQFLMALALGMRPAKIYTGQDSAVEGILL
HHHHHHHHHHCCCCEEHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
MDGSGEVLCYHKSEKPVMEDFLFLNTRLEKGSLDKDKYGFLERENGTYYFKLNAKIGLVK
ECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEEECEEEECC
R
C
>Mature Secondary Structure 
AFEATKREWCELYTFFRLLTEGKVTLGTAKAKKGEIDWPIAMIQREEHDGTRCYYIEKE
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEEEE
MVRIKGENSEKFVPREDFGIVADLILQAVKSSSEDEVTSPDGVEEFLDEVAIFDLEAKTE
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHEEEEECCCC
DRTDFYIAFWHPEAPLSGFSVRSRLGAMNPLLDGGRAANLKLEQSGVKFATPTVNKINAL
CCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCEECEEEECCCCEEECCCHHHHHCC
PEAPNEVAERMLLIERLGGVLKYSDVADRVFRSNLLMIDLHFPRVLTEMVRIMHLDDITR
CCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHH
ISELTEVIKQMNPLKIKDELVNKHGFYEFKVKQFLMALALGMRPAKIYTGQDSAVEGILL
HHHHHHHHHHCCCCEEHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
MDGSGEVLCYHKSEKPVMEDFLFLNTRLEKGSLDKDKYGFLERENGTYYFKLNAKIGLVK
ECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCEEEEEEECEEEECC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7514149 [H]