Click here to switch to the map view.

The map label for this gene is miaE [H]

Identifier: 29144750

GI number: 29144750

Start: 4652359

End: 4653123

Strand: Direct

Name: miaE [H]

Synonym: t4505

Alternate gene names: 29144750

Gene position: 4652359-4653123 (Clockwise)

Preceding gene: 29144749

Following gene: 29144754

Centisome position: 97.09

GC content: 55.03

Gene sequence:

>765_bases
ATGAATTACCCGCAAATACTCTCTCCGGTGCTTAATTTCCTGCATTGCCCGACGCCGCAGGCGTGGATTGTCCAGGCCCG
CGATCCGCAAAATCTTCCTCTTCTGCTTACCGATCACTTGATCTGCGAACTGAAGGCTGCGCAAACCGCGTTGCTGTTGG
TGCGTAAATATGTCGCCGATAAATCGGGCGCCGACGCGCTGCTCGCCTGGCTGCAACCTTACGAGGCGTTCGCGTTTCGT
CAGGGGCCGGAGCCGGATTTTGTTGCCCTGCATAAACAGATTAGCAAAAGCGCCATGCCGCAAACTGATGACCCCTGGGG
TCGTCAGCTTATCGACCGTATGGTGTTATTGATTAAAGAGGAGTTGCACCATTTTTGGCAGGTACGCGAGGTCATGCAGG
CCAGAAACATTCCTTATGTGAAAATCACCGCCAGCCGCTACGCAAAAGGAATGTTGAAAGCGGTACGCACGCACGAACCG
CTCACCTTAATTGATAAGCTGATTTGCGGGGCGTATATCGAAGCCCGCTCCTGCGAACGTTTTGCCGCTCTGGCCCCCTG
GCTGGATGAGGATTTACAGACGTTTTATCTCTCGCTTCTGCGCTCCGAAGCGCGGCATTATCAGGATTACCTGGCGCTGG
CGCAGCAGATTTCCGCCGAGGACATTTCTGCGCGCGTGCGCTATTTTGGCGACGTGGAAGCTGACCTTATCTTGTCGCCA
GACCGCGAGTTTCGTTTTCATAGCGGCGTGCCAGCCGCCGGATAA

Upstream 100 bases:

>100_bases
GACGATTATGTCGATGAAGATGACGACGGCGTGCGTCACTAAAGCTGCATAGTATGACGGTACGGCAGCGTCTGCTGTCG
TATTTTTTTACAGGTTACGT

Downstream 100 bases:

>100_bases
AGCGTAAAATTAAAGATTAGCGTTTTTATCTTTTACGCTGTCGCCGTCTGCAAAATTTGTGGTGGTCATCCGCCCGGCAT
CAAGCTGCTGGCGAGCAGGA

Product: tRNA hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGADALLAWLQPYEAFAFR
QGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEP
LTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGDVEADLILSP
DREFRFHSGVPAAG

Sequences:

>Translated_254_residues
MNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGADALLAWLQPYEAFAFR
QGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEP
LTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGDVEADLILSP
DREFRFHSGVPAAG
>Mature_254_residues
MNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVADKSGADALLAWLQPYEAFAFR
QGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKEELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEP
LTLIDKLICGAYIEARSCERFAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGDVEADLILSP
DREFRFHSGVPAAG

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 29028; Mature: 29028

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVAD
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
KSGADALLAWLQPYEAFAFRQGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCER
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGDVEADLILSP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DREFRFHSGVPAAG
CCCCCCCCCCCCCC
>Mature Secondary Structure
MNYPQILSPVLNFLHCPTPQAWIVQARDPQNLPLLLTDHLICELKAAQTALLLVRKYVAD
CCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
KSGADALLAWLQPYEAFAFRQGPEPDFVALHKQISKSAMPQTDDPWGRQLIDRMVLLIKE
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
ELHHFWQVREVMQARNIPYVKITASRYAKGMLKAVRTHEPLTLIDKLICGAYIEARSCER
HHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAALAPWLDEDLQTFYLSLLRSEARHYQDYLALAQQISAEDISARVRYFGDVEADLILSP
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DREFRFHSGVPAAG
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]