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The map label for this gene is pmbA [H]

Identifier: 29144717

GI number: 29144717

Start: 4620793

End: 4622145

Strand: Direct

Name: pmbA [H]

Synonym: t4472

Alternate gene names: 29144717

Gene position: 4620793-4622145 (Clockwise)

Preceding gene: 29144715

Following gene: 29144718

Centisome position: 96.43

GC content: 55.73

Gene sequence:

>1353_bases
ATGGCATTTGCAATGAAAGTAATCTCACAAGTTGAAGCGCAGCGTAAGATTCTGGAAGAAGCAGTTTCGACAGCGCTGGA
GTTAGCCTCAGGCAAATCGGATGGGGCGGAAGTCGCCGTCAGCAAGACTACCGGCATTAGCGTCAGCACCCGTTATGGTG
AAGTAGAGAATGTCGAATTTAATAGCGACGGCGCGCTGGGAATTACGGTGTATCACCAGAACCGCAAAGGCAGCGCCTCT
TCGACGGATTTAAGCCCGCAGGCGATTGCCCGTACCGTGCAGGCCGCGCTGGATATCGCGCGTTACACGTCGCCGGACCC
GTGCGCAGGCGTGGCGGACAAAGAACTGCTGGCGTTTGACGCGCCGGATCTGGATTTGTTCCACCCGGCAGAGGTTACGC
CAGACGAAGCCATTGAACTGGCCGCGCGTGCGGAACAGGCTTCTCTAAAGGCGGATAAACGCATCACCAATACCGAAGGC
GGTAGCTTTAACAGCCATTACGGCATTAAAGTGTTCGGCAACAGCCACGGTATGTTGCAGGGATATTGCTCCACGCGCCA
TTCGCTCTCAAGCTGCGTCATCGCCGAAGAGAACGGCGATATGGAGCGTGATTACGCTTACACCATCGGCCGGGCAATGG
CGGATCTGCAAGCGCCGGAATGGGTCGGGGCCGAGTGCGCGCGCCGTACCTTATCCCGCCTGTCGCCGCGCAAGCTCTCC
ACCATGAAAGCGCCGGTCATTTTCGCCAATGAAGTGGCGACAGGGCTATTTGGTCATCTGGTCGGGGCGATTGCCGGCGG
CGCGGTATACCGTAAATCCACTTTTCTGCTCGACTCGCTGGGCAAACAAATTCTGCCGGAATGGTTAACCATCGAAGAAC
ACCCGCATCTGCTAAAAGGGCTGGCGTCTTCACCGTTCGACAGCGAAGGCGTGCGTACGGAGCGCCGGGACATTGTGAAG
GATGGCGTACTGACGCAATGGTTGCTGACCAACTATTCCGCCCGCAAACTGGGGCTGAAAAGTACCGGTCATGCTGGCGG
CATTCATAACTGGCGTATCGCCGGGCAGGGGCTGAACTTTGAGCAGTTACTGAAAGAGATGGGTACCGGCCTGGTGGTGA
CGGAACTGATGGGCCAGGGCGTCAGCGGTATTACCGGCGACTACTCCCGCGGCGCTGCGGGATTCTGGGTTGAAAATGGT
GAAATCCAGTACCCGGTAAGTGAAATTACCATTGCCGGTAATTTAAAAGAGATGTGGCGTAATATTGTCACCGTAGGCGA
TGATATTGAAACGCGCAGTAATATACAATGTGGTTCGGTATTGTTACCGGAGATGAAAATCGCCGGACAATAA

Upstream 100 bases:

>100_bases
GTCATGTCAGGCTCCTTAAAAAAAGAGGCTAATGTTACCAGTTAAGATGCGCACTGAAAAACGGTTCTCTGTTAGACTTC
AGAGAAACTCTCTCTACATT

Downstream 100 bases:

>100_bases
TACCCGTCATACTTCAGGCTGCAGGCGCGTTGGCTGCCTGCAACTCGAATTATTCAGGGTATTACACTTATCGTTAATAA
TAAAAAGGAAGTGAGCAATG

Product: peptidase PmbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAFAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEG
GSFNSHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVK
DGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENG
EIQYPVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ

Sequences:

>Translated_450_residues
MAFAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSAS
STDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEG
GSFNSHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLS
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVK
DGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENG
EIQYPVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ
>Mature_449_residues
AFAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASS
TDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFDAPDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGG
SFNSHYGIKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLST
MKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKGLASSPFDSEGVRTERRDIVKD
GVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNFEQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGE
IQYPVSEITIAGNLKEMWRNIVTVGDDIETRSNIQCGSVLLPEMKIAGQ

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=450, Percent_Identity=95.7777777777778, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI1789640, Length=408, Percent_Identity=20.0980392156863, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48409; Mature: 48278

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEE
NSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFNSHYGIKVFGNSHGMLQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEE
GYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLS
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
LASSPFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCH
EQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKEMWR
HHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHH
NIVTVGDDIETRSNIQCGSVLLPEMKIAGQ
HHHCCCCCCCCCCCCCCCCEECCCHHCCCC
>Mature Secondary Structure 
AFAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTTGISVSTRYGEVENVEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEE
NSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARYTSPDPCAGVADKELLAFD
CCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
APDLDLFHPAEVTPDEAIELAARAEQASLKADKRITNTEGGSFNSHYGIKVFGNSHGMLQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCEE
GYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMADLQAPEWVGAECARRTLSRLSPRKLS
HHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
TMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFLLDSLGKQILPEWLTIEEHPHLLKG
HHCCCEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHH
LASSPFDSEGVRTERRDIVKDGVLTQWLLTNYSARKLGLKSTGHAGGIHNWRIAGQGLNF
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCEEEECCCCCH
EQLLKEMGTGLVVTELMGQGVSGITGDYSRGAAGFWVENGEIQYPVSEITIAGNLKEMWR
HHHHHHHCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEECCEEEECHHHEEEECCHHHHHH
NIVTVGDDIETRSNIQCGSVLLPEMKIAGQ
HHHCCCCCCCCCCCCCCCCEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]