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The map label for this gene is amiB [H]

Identifier: 29144656

GI number: 29144656

Start: 4559615

End: 4560934

Strand: Direct

Name: amiB [H]

Synonym: t4409

Alternate gene names: 29144656

Gene position: 4559615-4560934 (Clockwise)

Preceding gene: 29144655

Following gene: 29144657

Centisome position: 95.15

GC content: 57.65

Gene sequence:

>1320_bases
ATGATTTATCGCATCAAAAATGCCGTAATAGCAGCCTTAATCCTGCTGTGCGCGCAGGCGGGCGCCGCCAGTCTCTCTGA
TATTCAGGTGTCCAACGGCGAGCAGCAGGCGCGCATTACGCTGAGTTTTATCGGCGAGCCGGAATATGCTTACTCACAGG
ACGGTAAACGGACCGTGGCGCTGGATATCAGGCAGACCGGCGTGATTCAGGGGCTGCCGCTTCAGTTTAGCGGTAACAAT
CTGGTAAAAACGATCCGTGCCGGAACGCCGAAAGATGCGCAATCTTTACGGCTGCTTGTCGATCTGACGGAAAATGGTAA
AACCGAGGCGGTGAAGCGGCAAAACGGCGGCAACTATACGGTCATTTTCACCATTAATGCCGATGTTCCGCCGCCGCCAC
CGCCTGTCGTCGCGAAACGGGTTGAAAGCGCGCCCCGCCCGACGGAGCCTGCCCGTAATCCGTTTAAATCTTCTGATGAT
CGCCTGACCGGCGTAACCAGCAGCAACACCGTTACGCGGCCTGCGGCGCGCGCGTCGGCAGGCGCTGGCGATAAAGTGGT
GATTGCTATTGATGCCGGACATGGAGGCCAGGACCCCGGTGCGATAGGCCCGGGCGGTACGCGCGAGAAAAATGTCACCA
TCGCCATCGCGCGTAAGTTACGCACATTGCTGAATAACGATCCGATGTTTAAAGGCGTGCTGACGCGTGACGGCGACTAT
TTTATTTCGGTGATGGGGCGGTCTGATGTGGCGCGTAAGCAAAACGCCAACTTCCTTGTCTCTATTCATGCGGATGCCGC
GCCAAATCGCGACGCCACCGGCGCCTCCGTCTGGGTATTGTCCAATCGTCGCGCGAATAGCGAAATGGCAAACTGGCTTG
AGCAGCATGAGAAGCAATCCGAACTGTTAGGCGGCGCTGGCGATGTGCTGACGAACAGTCAGTCAGACCCTTACCTGAGC
CAGGCGGTACTGGATTTGCAATTCGGTCATTCGCAGCGGGTAGGGTATGATGTGGCGACGAACGTACTAAGCCAACTCGA
CGGCGTGGGGTCGCTGCATAAACGTCGCCCGGAACACGCGAGCCTGGGCGTGCTGCGTTCGCCGGATATCCCGTCCATTT
TGGTGGAGACGGGTTTTATCAGTAATCACGGCGAAGAACGATTGCTGGCGAGCGACCGCTATCAGCAGCAGATTGCTGAC
GCTATATACCGGGGGTTGCGTAAGTACTTTGCAGCGCATCCGATCCAGTCTGCGCCGCAGGGCAGCCCAGGCCAGACGGC
CAGTACCAACCAGCCTGGCGCTATAACCGCCGCGAATTAA

Upstream 100 bases:

>100_bases
GATTACCAGGCGCAGGGGCGTGAGGCTCGCGTAAGCGCGGTATCCTCATCGGGTCATTCGTTACTGGCGCGTTTAGCCGA
TTAGCTATAAAGGTGGCGGG

Downstream 100 bases:

>100_bases
GGAGATTTCATGCCGATTCAGGTTCTGCCGCCGCAGCTTGCGAACCAAATCGCCGCTGGCGAAGTGGTGGAACGCCCTGC
GTCGGTTGTTAAAGAGCTGG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLTNSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGSPGQTASTNQPGAITAAN

Sequences:

>Translated_439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLTNSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGSPGQTASTNQPGAITAAN
>Mature_439_residues
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVALDIRQTGVIQGLPLQFSGNN
LVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYTVIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDD
RLTGVTSSNTVTRPAARASAGAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQSELLGGAGDVLTNSQSDPYLS
QAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHASLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIAD
AIYRGLRKYFAAHPIQSAPQGSPGQTASTNQPGAITAAN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=435, Percent_Identity=83.2183908045977, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI87082163, Length=232, Percent_Identity=41.3793103448276, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788776, Length=246, Percent_Identity=40.2439024390244, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46850; Mature: 46850

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVA
CEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIRQTGVIQGLPLQFSGNNLVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYT
EEEECCCCEECCCEEECCCHHEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCEE
VIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASA
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCC
GAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCE
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQS
EEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ELLGGAGDVLTNSQSDPYLSQAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHA
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
SLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIADAIYRGLRKYFAAHPIQSAPQ
CCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSPGQTASTNQPGAITAAN
CCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MIYRIKNAVIAALILLCAQAGAASLSDIQVSNGEQQARITLSFIGEPEYAYSQDGKRTVA
CEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEEEECCCCCCCCCCCCEEEE
LDIRQTGVIQGLPLQFSGNNLVKTIRAGTPKDAQSLRLLVDLTENGKTEAVKRQNGGNYT
EEEECCCCEECCCEEECCCHHEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCEE
VIFTINADVPPPPPPVVAKRVESAPRPTEPARNPFKSSDDRLTGVTSSNTVTRPAARASA
EEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHCC
GAGDKVVIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFKGVLTRDGDY
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEEEEEECCCCE
FISVMGRSDVARKQNANFLVSIHADAAPNRDATGASVWVLSNRRANSEMANWLEQHEKQS
EEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ELLGGAGDVLTNSQSDPYLSQAVLDLQFGHSQRVGYDVATNVLSQLDGVGSLHKRRPEHA
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCCC
SLGVLRSPDIPSILVETGFISNHGEERLLASDRYQQQIADAIYRGLRKYFAAHPIQSAPQ
CCCEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GSPGQTASTNQPGAITAAN
CCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]