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The map label for this gene is artJ [H]

Identifier: 29144652

GI number: 29144652

Start: 4554888

End: 4555607

Strand: Reverse

Name: artJ [H]

Synonym: t4403

Alternate gene names: 29144652

Gene position: 4555607-4554888 (Counterclockwise)

Preceding gene: 29144653

Following gene: 161486770

Centisome position: 95.07

GC content: 49.44

Gene sequence:

>720_bases
ATGCTGTTAGCCAGCCTCTCCGTTCACGCTGCTTCCGTTTCCGCCAGAACATTACATTTTGGCACCTCCGCCACCTATGC
GCCCTATGAGTTTGTCGATGCCGATAATAAAATTGTTGGTTTCGATATTGATGTCGCCAACGCGGTCTGCAAAGAGATGC
AAGCGGAGTGCTCATTCACTAACCAGAGCTTTGATAGTCTGATCCCCGGCCTACGTTTCAAAAAGTTTGATGCGGTGATC
GCCGGTATGGATATGACGCCCAAACGTGAGCAGCAGGTCTCCTTTAGCCAACCCTATTACGAAGGTTTATCGGCGGTAGT
CGTCACGCGTAAAGGGGCTTATCACACTTTTGCCGATCTGAAAGGCAAAAAAGTCGGACTGGAAAACGGTACAACGCATC
AGCGCTATTTGCAGGATAAACAACAAGCTATCACGCCTGTCGCCTATGACAGCTATCTAAACGCTTTTACCGATCTGAAA
AATAACCGTCTTGAGGGCGTGTTTGGCGATGTCGCCGCGATTGGTAAGTGGCTAAAAAACAATCCGGATTACGCCATCAT
GGATGAACGCGCCAGCGATCCTGACTACTACGGAAAAGGGTTAGGCATTGCGGTACGCAAAGGCAACGACGCGCTATTGC
AAGAAATTAATGCCGCGCTCGACAAGGTGAAAGCGTCGCCGGAATATGCGCAAATGCAGGAGAAATGGTTTACGCAGTAA

Upstream 100 bases:

>100_bases
GCACAGAATCAGAATAGCACCCTGCGCCAAAAAAAGAATAGCACGGTGACCACAACATCCAATTGATATCAGGGATCAAG
ATGAAAAAAAACTTATTGTC

Downstream 100 bases:

>100_bases
TTCAGTGGCGGGAGAGCTGCCGGATGAGACGCTCTGGCGTTGCCATCCGGCATGTTACGCCTCACAGCTTAATGAAATGC
TCGCGGTAGTAAGCCAGCTC

Product: arginine-binding periplasmic protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRFKKFDAVI
AGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLK
NNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQEKWFTQ

Sequences:

>Translated_239_residues
MLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRFKKFDAVI
AGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLK
NNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQEKWFTQ
>Mature_239_residues
MLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFTNQSFDSLIPGLRFKKFDAVI
AGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADLKGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLK
NNRLEGVFGDVAAIGKWLKNNPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQEKWFTQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=238, Percent_Identity=57.1428571428571, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1787088, Length=236, Percent_Identity=52.9661016949153, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788649, Length=244, Percent_Identity=35.655737704918, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=32.8947368421053, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1788228, Length=224, Percent_Identity=31.25, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787031, Length=236, Percent_Identity=29.2372881355932, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26479; Mature: 26479

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFT
CEEECCCHHHHHCEEEEEEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHCCC
NQSFDSLIPGLRFKKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADL
CCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCEEEEEECCCCCHHHHHC
KGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLKNNRLEGVFGDVAAIGKWLKN
CCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQEKWFTQ
CCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLLASLSVHAASVSARTLHFGTSATYAPYEFVDADNKIVGFDIDVANAVCKEMQAECSFT
CEEECCCHHHHHCEEEEEEECCCCCCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHCCC
NQSFDSLIPGLRFKKFDAVIAGMDMTPKREQQVSFSQPYYEGLSAVVVTRKGAYHTFADL
CCCHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCEEEEEECCCCCHHHHHC
KGKKVGLENGTTHQRYLQDKQQAITPVAYDSYLNAFTDLKNNRLEGVFGDVAAIGKWLKN
CCCEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
NPDYAIMDERASDPDYYGKGLGIAVRKGNDALLQEINAALDKVKASPEYAQMQEKWFTQ
CCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]