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The map label for this gene is dcuA [H]

Identifier: 29144625

GI number: 29144625

Start: 4528925

End: 4530226

Strand: Reverse

Name: dcuA [H]

Synonym: t4376

Alternate gene names: 29144625

Gene position: 4530226-4528925 (Counterclockwise)

Preceding gene: 29144626

Following gene: 29144624

Centisome position: 94.54

GC content: 53.92

Gene sequence:

>1302_bases
ATGATAGTTGTCGAACTTATCATTGTTTTGCTGGCAATCTTTTTGGGCGCCAGACTTGGGGGCATCGGTATTGGATTCGC
AGGTGGGCTGGGTGTTCTGGTACTTGCCGCCATCGGCGTGAAGCCAGGGACAATTCCATTCGATGTCATTTCCATCATCA
TGGCGGTTATCGCCGCGATCTCTGCTATGCAGGTCGCGGGCGGTCTGGATTATTTGGTAAACCAGACAGAAAAACTGCTG
CGTAAGAACCCGAAATACATCACTATCCTCGCACCGATTGTGACCTATTTCCTGACTATTTTTGCTGGTACCGGGAACAT
TTCCCTGGCAACGCTGCCGGTTATTGCTGAAGTCGCGAAGGAGCAGGGAATCAAACCTTGCCGTCCGCTCTCAACCGCCG
TGGTATCCGCGCAGATTGCGATTACCGCCTCGCCGATTTCTGCGGCTGTGGTGTATATGTCTTCTGTTATGGAAGGTCAT
GGCATTAGCTACATTCACCTGCTGTCCGTGGTTATCCCCTCTACGCTGCTGGCGGTGCTGGTGATGTCCTTCCTGGTCAC
GATGCTGTTCAACTCCAAACTGTCGGACGACCCGATTTACCGCAAGCGTCTGGAAGAAGGGCTGATTGAACTGCGTGGTG
AAAAGCAGATTGAAATCAAACCGATGGCAAAAAATTCCGTCTGGCTGTTCCTGCTGGGCGTTATCTGCGTGGTTGTTTAT
GCGATTGTCAACAGTCCGAGCCTCGGCCTGGTTGAGAAACCGCTGATGAACACCACCAACGCTATTTTGATCATCATGTT
GAGCGTCGCAACGCTGACCACCATCCTGTGTAAAGTGGAAACTGACGCCATCCTCAACTCCAGTACCTTTAAAGCAGGGA
TGAGCGCCTGTATTTGTATTCTGGGCGTGGCGTGGCTGGGCGATACCTTCGTATCCGCTAACATCGACTGGATCAAAGAC
ACTGCGGGTAGCGTCATCCAGGGGCATCCGTGGCTGCTGGCCGTCATCTTCTTCTTTGCTTCCGCGCTGCTCTACTCTCA
GGCGGCAACGGCGAAAGCGCTGATGCCGATGGCGCTGGCGCTGAACGTCTCTCCGCTGACGGCAGTCGCTTCCTTCGCCG
CCGTTTCCGGCCTGTTCATTTTGCCGACCTACCCGACTCTGGTTGCGGCGGTACAGATGGATGACACCGGTACAACCCGT
ATCGGTAAATTCGTCTTTAACCACCCGTTCTTCATCCCAGGCACCCTGGGCGTCGTTCTGGCTATCTGCTCCGGCTTCCT
GCTGGGCAGCTTTATGCTGTAA

Upstream 100 bases:

>100_bases
ACCAAAGAGGCACGTCACGATGACGTGCCTTTTTTCTTACAAGAAGTTACCAAATAACAACAATTTAATATCAACTTGTT
AAACAACAAGGAAGGCTAAT

Downstream 100 bases:

>100_bases
GTGTTTCACCCGCGGGGCGTATCACGCCCCGCATTCTCCCGCCCTTTGACTAACATCCTGCCCTCGTCCGTTGTATAGTG
ACCGTTCACTTGCGGGTCTT

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGTIPFDVISIIMAVIAAISAMQVAGGLDYLVNQTEKLL
RKNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYIHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLIELRGEKQIEIKPMAKNSVWLFLLGVICVVVY
AIVNSPSLGLVEKPLMNTTNAILIIMLSVATLTTILCKVETDAILNSSTFKAGMSACICILGVAWLGDTFVSANIDWIKD
TAGSVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVVLAICSGFLLGSFML

Sequences:

>Translated_433_residues
MIVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGTIPFDVISIIMAVIAAISAMQVAGGLDYLVNQTEKLL
RKNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYIHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLIELRGEKQIEIKPMAKNSVWLFLLGVICVVVY
AIVNSPSLGLVEKPLMNTTNAILIIMLSVATLTTILCKVETDAILNSSTFKAGMSACICILGVAWLGDTFVSANIDWIKD
TAGSVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVVLAICSGFLLGSFML
>Mature_433_residues
MIVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGTIPFDVISIIMAVIAAISAMQVAGGLDYLVNQTEKLL
RKNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAKEQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
GISYIHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLIELRGEKQIEIKPMAKNSVWLFLLGVICVVVY
AIVNSPSLGLVEKPLMNTTNAILIIMLSVATLTTILCKVETDAILNSSTFKAGMSACICILGVAWLGDTFVSANIDWIKD
TAGSVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTR
IGKFVFNHPFFIPGTLGVVLAICSGFLLGSFML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=433, Percent_Identity=94.2263279445727, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1790565, Length=404, Percent_Identity=36.3861386138614, Blast_Score=236, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45723; Mature: 45723

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGTIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVNQTEKLLRKNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYIHLLSVVIPSTLLAVL
HCCCCCCCCHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLIELRGEKQIEIKPMAKNSVWLFLLGVICVVVY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AIVNSPSLGLVEKPLMNTTNAILIIMLSVATLTTILCKVETDAILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGVAWLGDTFVSANIDWIKDTAGSVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHCCHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVVL
CCCCHHHHHHHHHHHCCEEEECCCHHHEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AICSGFLLGSFML
HHHHHHHHHHHCC
>Mature Secondary Structure
MIVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGTIPFDVISIIMAVIAAI
CHHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
SAMQVAGGLDYLVNQTEKLLRKNPKYITILAPIVTYFLTIFAGTGNISLATLPVIAEVAK
HHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
EQGIKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGHGISYIHLLSVVIPSTLLAVL
HCCCCCCCCHHHHHHHEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMSFLVTMLFNSKLSDDPIYRKRLEEGLIELRGEKQIEIKPMAKNSVWLFLLGVICVVVY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AIVNSPSLGLVEKPLMNTTNAILIIMLSVATLTTILCKVETDAILNSSTFKAGMSACICI
HHHCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGVAWLGDTFVSANIDWIKDTAGSVIQGHPWLLAVIFFFASALLYSQAATAKALMPMALA
HHHHHHCCHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVSPLTAVASFAAVSGLFILPTYPTLVAAVQMDDTGTTRIGKFVFNHPFFIPGTLGVVL
CCCCHHHHHHHHHHHCCEEEECCCHHHEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHH
AICSGFLLGSFML
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]