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The map label for this gene is nucD [H]

Identifier: 29144567

GI number: 29144567

Start: 4472612

End: 4473082

Strand: Reverse

Name: nucD [H]

Synonym: t4314

Alternate gene names: 29144567

Gene position: 4473082-4472612 (Counterclockwise)

Preceding gene: 29144568

Following gene: 29144566

Centisome position: 93.35

GC content: 60.3

Gene sequence:

>471_bases
GTGCTGGCTATCGCAGCCACGCTGCCCGGATTTCAGTCGCTTCATACCTCCGTTGAGGGGCTGAAACTGATCGCCGATTA
CGAGGGATGCCGCCTGCAGCCTTATCAGTGTAGCGCGGGCGTCTGGACTGACGGGATCGGCAATACGTCCGGTGTGGTGC
CTGGAAAAACCATCACGGAACGGCAGGCGGCGCAGGGACTTATCACCAATGTGCTGCGCGTGGAGCGGGCGCTGGAAAAA
TGTGTGGTGCAGCCGATGCCGCAAAAGGTCTATGACGCGGTGGTGTCGTTTGCTTTCAACGTGGGCACCGGCAACGCCTG
CAGCTCCACGCTGGTTAAGTTGCTGAACCAGCGGCGCTGGGCGGATGCCTGCCATCAGCTGCCGCGCTGGGTATATGTCA
AAGGTGTGTTTAATCAGGGGCTGGACAACCGCCGCGCGCGGGAAATGGCCTGGTGCTTAAAAGGAGCCTAA

Upstream 100 bases:

>100_bases
TGGTACTACAAACACCAGTCTTTCAAATTGTTACGTGGCGGCAAGATTTCGCGGGGGGAATATGAATACTTCAATCGTTA
AGTGCTGCCTTGTCGGGGCA

Downstream 100 bases:

>100_bases
CGGAATGAAAAAGAAAGTCATGAGCGTTTTTTTCCAGCTGGCATGGGCTGCGCTGTTGGTAATCAGCCTGCTGTATCCGC
GCAGCGGTGCGCCGGTTCTG

Product: lysozyme

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MLAIAATLPGFQSLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVVPGKTITERQAAQGLITNVLRVERALEK
CVVQPMPQKVYDAVVSFAFNVGTGNACSSTLVKLLNQRRWADACHQLPRWVYVKGVFNQGLDNRRAREMAWCLKGA

Sequences:

>Translated_156_residues
MLAIAATLPGFQSLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVVPGKTITERQAAQGLITNVLRVERALEK
CVVQPMPQKVYDAVVSFAFNVGTGNACSSTLVKLLNQRRWADACHQLPRWVYVKGVFNQGLDNRRAREMAWCLKGA
>Mature_156_residues
MLAIAATLPGFQSLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVVPGKTITERQAAQGLITNVLRVERALEK
CVVQPMPQKVYDAVVSFAFNVGTGNACSSTLVKLLNQRRWADACHQLPRWVYVKGVFNQGLDNRRAREMAWCLKGA

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=129, Percent_Identity=36.4341085271318, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 17068; Mature: 17068

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAIAATLPGFQSLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVVPGKTITE
CEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQAAQGLITNVLRVERALEKCVVQPMPQKVYDAVVSFAFNVGTGNACSSTLVKLLNQRRW
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ADACHQLPRWVYVKGVFNQGLDNRRAREMAWCLKGA
HHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MLAIAATLPGFQSLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVVPGKTITE
CEEEECCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQAAQGLITNVLRVERALEKCVVQPMPQKVYDAVVSFAFNVGTGNACSSTLVKLLNQRRW
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ADACHQLPRWVYVKGVFNQGLDNRRAREMAWCLKGA
HHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]