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The map label for this gene is pilO

Identifier: 29144500

GI number: 29144500

Start: 4411887

End: 4413182

Strand: Direct

Name: pilO

Synonym: t4243

Alternate gene names: NA

Gene position: 4411887-4413182 (Clockwise)

Preceding gene: 29144499

Following gene: 29144501

Centisome position: 92.07

GC content: 56.56

Gene sequence:

>1296_bases
GTGGCTGATATGGCAGATATTCCGTTATCCGGCAAGCCCCGTACGCAGGTTCTGCGCTGGCAGCGCCAGAACTGGGTAGC
GGGGCTTCGCTGGGAAGAAAAGCAAAAGTCGGTACGTCGAGCCCCTGTACAACGTCGGGGTGTTCTGAACGTTGTGTTGA
CGGTGAAGCAGGGCAAGAGCCGGTTGACCGCGTTGGGGCAAATTGAGGCCGGCTGGCGTCACCAGAAGCTGTATTCCCTG
GCGGCGCTGTTCAGCCAGCAGTGTACACCCAATGGCTATGGGGTATACCAGCTTGATGATGGCAGACAGGTCTTTCTGGC
CACGGTGAAGGGGATGCCATCTCCGATGGCAGATGTGATCGGAACTCCAGATCAAATCATCCGGGCGAGAGAGCTCTTTC
TGTCCTTCAACCCTGCTCCTGAGGACGGATGGACGATACCTTCATCACCGGAATTCCCCTTGTCCTGGAACACTTTGGTC
GGTCAGGTATCTTCGGCACAGCTCCGGTTTGCCAGAGTGGCGGGCGTGTCTAATCCTTTGCGGCCATACGGGGCGGGAGT
GCTGTCAGTGGCTGCCCTGCTTGGTGGTGCAGTCTGGTGGAGCAGGTTACCCGAACCTCCGGTCGCGCCTGTCGTGCAGG
AGTTACCGGCACCGGTGCAGGACGTGTTTAAAAAAACACCTGAACCTGTTTACCTTCCACATCCCTGGGCAGATATGCCT
GTGGCCAGACACTTTCTGAACCGCTGTCAGCGCTGGCGCGCCGCAGTTCCTGTAAGTCTTGACCGTTGGCGTCTCGAACG
GATTCGTTGCAGTGCTGAGGGGCTGGAGACGGTGTACCAGCGGCAGCCCGGTGGGACGGCGGCAGGCTTTGCGGAGCGGG
TGAAGCAGGTTTTTAAGCGTACACCAGTATTCAACCTTGTTTCAGGTGGCAACGAAGGTGTGGTGTTTATTCCATGGGCG
AAATTCACATTACAGGATGAGACTGCGCCGGATGCGGGGACCCAGTTGATGCAGGCGGTGTCGTGGTTTCAGTCACGCCA
GGTCAGTTTTTCTCTGTCCGAAGTGAAAACTCCTCCCGCGATGCCCGGTGATGGTGCCGGCAACGATGCGCCCCAGCCGA
TACAGGACTGGCATGAATACACGTTCAGTATCACTGATAAACACATGCCTGAGTGGATTTTACAGGGACTCGATATGCAG
GGGGTACGGCTGAGTTCTGTGGGTTACACCCTGAGTCCTCAGGGACAGTTTACTTATCAGATTGAGGGGCATCTTTATGC
TTATGCCAAAAAATAG

Upstream 100 bases:

>100_bases
ACCTTTACCCCGGGTAATTTCCTGCTGGGTGGCGGTCGGGACGGCGATGATTCTCGTAGTACGCTGGTCATTATTATCAC
CCCGGAGCTGATGGGGAACG

Downstream 100 bases:

>100_bases
TGTTTTCATCAGTGGTGTTCTCCTGATGGCGTTCAGCGCAGGGGCATTTTCGCAGCCCACACTCGCAGTTCCTCCTTCCG
CAGAGGTGATTTCTGCAATG

Product: pilus assembly protein

Products: NA

Alternate protein names: Type IV Pilus Protein; PilO Type IV Pilus Biogenesis Outer Membrane Protein; PilO Protein; Pilus Assembly Protein; Type IV Pilus Biogenesis Outer Membrane Protein PilO; Type IV B Pilus Protein; Conjugal Transfer Protein; Type IV Secretion System; Type IV Pilus Accesory Protein

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MADMADIPLSGKPRTQVLRWQRQNWVAGLRWEEKQKSVRRAPVQRRGVLNVVLTVKQGKSRLTALGQIEAGWRHQKLYSL
AALFSQQCTPNGYGVYQLDDGRQVFLATVKGMPSPMADVIGTPDQIIRARELFLSFNPAPEDGWTIPSSPEFPLSWNTLV
GQVSSAQLRFARVAGVSNPLRPYGAGVLSVAALLGGAVWWSRLPEPPVAPVVQELPAPVQDVFKKTPEPVYLPHPWADMP
VARHFLNRCQRWRAAVPVSLDRWRLERIRCSAEGLETVYQRQPGGTAAGFAERVKQVFKRTPVFNLVSGGNEGVVFIPWA
KFTLQDETAPDAGTQLMQAVSWFQSRQVSFSLSEVKTPPAMPGDGAGNDAPQPIQDWHEYTFSITDKHMPEWILQGLDMQ
GVRLSSVGYTLSPQGQFTYQIEGHLYAYAKK

Sequences:

>Translated_431_residues
MADMADIPLSGKPRTQVLRWQRQNWVAGLRWEEKQKSVRRAPVQRRGVLNVVLTVKQGKSRLTALGQIEAGWRHQKLYSL
AALFSQQCTPNGYGVYQLDDGRQVFLATVKGMPSPMADVIGTPDQIIRARELFLSFNPAPEDGWTIPSSPEFPLSWNTLV
GQVSSAQLRFARVAGVSNPLRPYGAGVLSVAALLGGAVWWSRLPEPPVAPVVQELPAPVQDVFKKTPEPVYLPHPWADMP
VARHFLNRCQRWRAAVPVSLDRWRLERIRCSAEGLETVYQRQPGGTAAGFAERVKQVFKRTPVFNLVSGGNEGVVFIPWA
KFTLQDETAPDAGTQLMQAVSWFQSRQVSFSLSEVKTPPAMPGDGAGNDAPQPIQDWHEYTFSITDKHMPEWILQGLDMQ
GVRLSSVGYTLSPQGQFTYQIEGHLYAYAKK
>Mature_430_residues
ADMADIPLSGKPRTQVLRWQRQNWVAGLRWEEKQKSVRRAPVQRRGVLNVVLTVKQGKSRLTALGQIEAGWRHQKLYSLA
ALFSQQCTPNGYGVYQLDDGRQVFLATVKGMPSPMADVIGTPDQIIRARELFLSFNPAPEDGWTIPSSPEFPLSWNTLVG
QVSSAQLRFARVAGVSNPLRPYGAGVLSVAALLGGAVWWSRLPEPPVAPVVQELPAPVQDVFKKTPEPVYLPHPWADMPV
ARHFLNRCQRWRAAVPVSLDRWRLERIRCSAEGLETVYQRQPGGTAAGFAERVKQVFKRTPVFNLVSGGNEGVVFIPWAK
FTLQDETAPDAGTQLMQAVSWFQSRQVSFSLSEVKTPPAMPGDGAGNDAPQPIQDWHEYTFSITDKHMPEWILQGLDMQG
VRLSSVGYTLSPQGQFTYQIEGHLYAYAKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 48054; Mature: 47923

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADMADIPLSGKPRTQVLRWQRQNWVAGLRWEEKQKSVRRAPVQRRGVLNVVLTVKQGKS
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCEEEEEEEEECCCC
RLTALGQIEAGWRHQKLYSLAALFSQQCTPNGYGVYQLDDGRQVFLATVKGMPSPMADVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHC
GTPDQIIRARELFLSFNPAPEDGWTIPSSPEFPLSWNTLVGQVSSAQLRFARVAGVSNPL
CCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCC
RPYGAGVLSVAALLGGAVWWSRLPEPPVAPVVQELPAPVQDVFKKTPEPVYLPHPWADMP
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCCCCEECCCCCCCCH
VARHFLNRCQRWRAAVPVSLDRWRLERIRCSAEGLETVYQRQPGGTAAGFAERVKQVFKR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TPVFNLVSGGNEGVVFIPWAKFTLQDETAPDAGTQLMQAVSWFQSRQVSFSLSEVKTPPA
CCCEEEECCCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCC
MPGDGAGNDAPQPIQDWHEYTFSITDKHMPEWILQGLDMQGVRLSSVGYTLSPQGQFTYQ
CCCCCCCCCCCCHHHHHHHEEEEECCCCCHHHHHCCCCCCCEEEECCCEEECCCCCEEEE
IEGHLYAYAKK
ECCEEEEEECC
>Mature Secondary Structure 
ADMADIPLSGKPRTQVLRWQRQNWVAGLRWEEKQKSVRRAPVQRRGVLNVVLTVKQGKS
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHCCEEEEEEEEECCCC
RLTALGQIEAGWRHQKLYSLAALFSQQCTPNGYGVYQLDDGRQVFLATVKGMPSPMADVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCHHHHC
GTPDQIIRARELFLSFNPAPEDGWTIPSSPEFPLSWNTLVGQVSSAQLRFARVAGVSNPL
CCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCC
RPYGAGVLSVAALLGGAVWWSRLPEPPVAPVVQELPAPVQDVFKKTPEPVYLPHPWADMP
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHCCCCCEECCCCCCCCH
VARHFLNRCQRWRAAVPVSLDRWRLERIRCSAEGLETVYQRQPGGTAAGFAERVKQVFKR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TPVFNLVSGGNEGVVFIPWAKFTLQDETAPDAGTQLMQAVSWFQSRQVSFSLSEVKTPPA
CCCEEEECCCCCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEHHHCCCCCC
MPGDGAGNDAPQPIQDWHEYTFSITDKHMPEWILQGLDMQGVRLSSVGYTLSPQGQFTYQ
CCCCCCCCCCCCHHHHHHHEEEEECCCCCHHHHHCCCCCCCEEEECCCEEECCCCCEEEE
IEGHLYAYAKK
ECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA