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The map label for this gene is topB [H]

Identifier: 29144494

GI number: 29144494

Start: 4403378

End: 4405372

Strand: Direct

Name: topB [H]

Synonym: t4234

Alternate gene names: 29144494

Gene position: 4403378-4405372 (Clockwise)

Preceding gene: 29144493

Following gene: 29144495

Centisome position: 91.89

GC content: 52.88

Gene sequence:

>1995_bases
ATGCGTCTGGTGTTGTGTGAAAAACCTTCTCAGGGCAGAGATATTGCGAAGTTTCTTGGCGCCACTCAAAGAGGCGAAGG
ATTCCTGAATGGTCCGGGAGTTACAGTCACCTGGGCTCGCGGCCATCTGCTGGAGACAGCTGAACCGGAAGTATATGGCG
AACAGTATGGTAAGCCATGGCGCACCGATGTGCTTCCTTTATTACCACAGCAGTGGAAACTGGTAGTCAAAGCGGATGCA
AAAGCCCAATTCGCGGTAATTAACCGTCTGCTTAAACAGGTAGATGAGGTGGTAATTGCCACGGATGCTGACCGGGAAGG
TGAAGTAATCGCCCGTGAGTTGCTGGATTACTGCAAGTTTCAGGGGCGTGTATTCCGGTTGTGGATGTCAGCTCTGGATG
ATGCCAGTATCCGGTCAGCGTTAGCTGATTTGTGGCCGTCTTCCCGAACTGAATCGCTTTATTATGCTGGCGTGGGCAGG
AGCAGGGCTGACTGGCTGATCGGTATGAATCTGACTCGCCTGTTTACACTCCTGGGGCGTGAAGCGGGTATCGGTAACGT
ACTTTCAGTTGGCCGGGTACAAACGCCAACGCTGGCGATAGTGGTAAAGCGTGATCGTGAAATTGATAACTTCGTGTCGA
AACCGTACTTCGAAGTTATTGCCACGATGGCCGTGAATGGCGTGTTGTTTCCATCCAAATGGGTACCGGCTGCACAGTAT
TGCGATGAAGAGAAGCGTTGTATCCAGTCGGGGGTAGCGGCACAGGTGGTTCAGTTGTGTCGGCAAACGCCAACGGCTGC
GATAATCAATTGCCAGATCGAGAGGAAAAAACAATCAGCGCCGCTGGCTTTCAGCCTGAGCACACTACAACAGGCCTGCT
CCCGACACTGGGGGATGCCAGCCCAGACGGTACTTGATATCGCCCAGAAGTTATATGAAACCCACAAACTGACGACCTAT
CCCAGAACTGACTGTGGTTATCTGCCGTTATCGATGCGAGAAGAAATCCCACAGGTACTGTCTGCTGTGGTCGGAACTGA
TCCGGCGCTACAGCCGGTGGTCGCGAGTCTGGACACTCAGTTTGTGTCACGTATCTGGAATGATAAAAAAATTACCGCTC
ATCACGGTATCATTCCGACAAAACATACTGGTGATCTGAGCAAACTCAGTGATGCTGAGCGCAATGTCTATCAGCTGGTC
AGGTTGCACTACCTGGCCCAGTTCCTGCCCCAGATGGAAATTGACGTGACTGAGGCCACATTTAATATTGGCGGTCAGCT
TTTCCGTACCCGTGGTAACGTTTTGGTGAAACCCGGCTGGAAATCTCTGTTTGGCAAAATGTCCGAAGATGATGAGGATA
ATACTGGTGAAACCACACTGCCCGCCATGCAGGCGGGCCAGGTCTGCCAGGTGCAGGGCGCTGAGCAGAAGGTACTGCAG
ACAAAACCGCCTGCTCCGTACAACGATGGAACTCTTATTGCCGCGATGACCAATGCGGCGGCGTTCGTTACTGACGCCGC
GCTGAAAAAGGTGCTCAAAGAGAATGCGGGGATAGGAACTGAAGCAACACGGGCCGGGATAATCGACACGCTGTTGAAGC
GTGGGTTTCTGGTGCGTGAGAAAAAGACGCTTAAGTCAACACCTCTTGGACGTAACCTGGTGGATGTGTTGCCAGGAACG
TTAACGAATCCCGGACTTACGGCACTGTGGGAACAGATGCTGGATGAGGTAGCTGCAGGCAGGGTGAGCCTGGATGACTT
TATGGCAAAGCAAAGTCAGTGGGTCAGTCAGCTGGTCGCTCAGGGGAGATCACAGCCACTGACTATGCAGGCGCCACCTT
CACCACCCTGTCCTGTATGTGGCGGCAAGACGTTGCAGCGCAATGGAAAGAGTGGCTTGTTCTGGGGCTGCCTGAAATAT
CCGGAGTGCAAAGGTATCGTGAACAATGGTAACAAGGGCCCCAGAGGACGCAGACGAAAACCTTCGCCAGCCTAG

Upstream 100 bases:

>100_bases
AAGAACAGATTGTGGCTGACCCTTCTTTATTTGTCGCTGATTCGAAACGCATCGATGGTTAAGCCCGCAGAAAGGCTGGT
TTTCTGAAAGGAGTGTTATC

Downstream 100 bases:

>100_bases
AATACTAACGGGTTGTTTTCTTTCGCTGAATTCTGGTAGCCTTATATCGCTTTGATAAGAGCAGCACCCATGGCTGTGGA
ACGGTTCGAAAGCTTTGCAT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 664; Mature: 664

Protein sequence:

>664_residues
MRLVLCEKPSQGRDIAKFLGATQRGEGFLNGPGVTVTWARGHLLETAEPEVYGEQYGKPWRTDVLPLLPQQWKLVVKADA
KAQFAVINRLLKQVDEVVIATDADREGEVIARELLDYCKFQGRVFRLWMSALDDASIRSALADLWPSSRTESLYYAGVGR
SRADWLIGMNLTRLFTLLGREAGIGNVLSVGRVQTPTLAIVVKRDREIDNFVSKPYFEVIATMAVNGVLFPSKWVPAAQY
CDEEKRCIQSGVAAQVVQLCRQTPTAAIINCQIERKKQSAPLAFSLSTLQQACSRHWGMPAQTVLDIAQKLYETHKLTTY
PRTDCGYLPLSMREEIPQVLSAVVGTDPALQPVVASLDTQFVSRIWNDKKITAHHGIIPTKHTGDLSKLSDAERNVYQLV
RLHYLAQFLPQMEIDVTEATFNIGGQLFRTRGNVLVKPGWKSLFGKMSEDDEDNTGETTLPAMQAGQVCQVQGAEQKVLQ
TKPPAPYNDGTLIAAMTNAAAFVTDAALKKVLKENAGIGTEATRAGIIDTLLKRGFLVREKKTLKSTPLGRNLVDVLPGT
LTNPGLTALWEQMLDEVAAGRVSLDDFMAKQSQWVSQLVAQGRSQPLTMQAPPSPPCPVCGGKTLQRNGKSGLFWGCLKY
PECKGIVNNGNKGPRGRRRKPSPA

Sequences:

>Translated_664_residues
MRLVLCEKPSQGRDIAKFLGATQRGEGFLNGPGVTVTWARGHLLETAEPEVYGEQYGKPWRTDVLPLLPQQWKLVVKADA
KAQFAVINRLLKQVDEVVIATDADREGEVIARELLDYCKFQGRVFRLWMSALDDASIRSALADLWPSSRTESLYYAGVGR
SRADWLIGMNLTRLFTLLGREAGIGNVLSVGRVQTPTLAIVVKRDREIDNFVSKPYFEVIATMAVNGVLFPSKWVPAAQY
CDEEKRCIQSGVAAQVVQLCRQTPTAAIINCQIERKKQSAPLAFSLSTLQQACSRHWGMPAQTVLDIAQKLYETHKLTTY
PRTDCGYLPLSMREEIPQVLSAVVGTDPALQPVVASLDTQFVSRIWNDKKITAHHGIIPTKHTGDLSKLSDAERNVYQLV
RLHYLAQFLPQMEIDVTEATFNIGGQLFRTRGNVLVKPGWKSLFGKMSEDDEDNTGETTLPAMQAGQVCQVQGAEQKVLQ
TKPPAPYNDGTLIAAMTNAAAFVTDAALKKVLKENAGIGTEATRAGIIDTLLKRGFLVREKKTLKSTPLGRNLVDVLPGT
LTNPGLTALWEQMLDEVAAGRVSLDDFMAKQSQWVSQLVAQGRSQPLTMQAPPSPPCPVCGGKTLQRNGKSGLFWGCLKY
PECKGIVNNGNKGPRGRRRKPSPA
>Mature_664_residues
MRLVLCEKPSQGRDIAKFLGATQRGEGFLNGPGVTVTWARGHLLETAEPEVYGEQYGKPWRTDVLPLLPQQWKLVVKADA
KAQFAVINRLLKQVDEVVIATDADREGEVIARELLDYCKFQGRVFRLWMSALDDASIRSALADLWPSSRTESLYYAGVGR
SRADWLIGMNLTRLFTLLGREAGIGNVLSVGRVQTPTLAIVVKRDREIDNFVSKPYFEVIATMAVNGVLFPSKWVPAAQY
CDEEKRCIQSGVAAQVVQLCRQTPTAAIINCQIERKKQSAPLAFSLSTLQQACSRHWGMPAQTVLDIAQKLYETHKLTTY
PRTDCGYLPLSMREEIPQVLSAVVGTDPALQPVVASLDTQFVSRIWNDKKITAHHGIIPTKHTGDLSKLSDAERNVYQLV
RLHYLAQFLPQMEIDVTEATFNIGGQLFRTRGNVLVKPGWKSLFGKMSEDDEDNTGETTLPAMQAGQVCQVQGAEQKVLQ
TKPPAPYNDGTLIAAMTNAAAFVTDAALKKVLKENAGIGTEATRAGIIDTLLKRGFLVREKKTLKSTPLGRNLVDVLPGT
LTNPGLTALWEQMLDEVAAGRVSLDDFMAKQSQWVSQLVAQGRSQPLTMQAPPSPPCPVCGGKTLQRNGKSGLFWGCLKY
PECKGIVNNGNKGPRGRRRKPSPA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=703, Percent_Identity=26.0312944523471, Blast_Score=135, Evalue=2e-31,
Organism=Homo sapiens, GI4507635, Length=687, Percent_Identity=25.0363901018923, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=40.7108239095315, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1787529, Length=682, Percent_Identity=25.5131964809384, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=516, Percent_Identity=25.5813953488372, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=638, Percent_Identity=24.7648902821317, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=664, Percent_Identity=25.4518072289157, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585251, Length=724, Percent_Identity=24.4475138121547, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640096, Length=522, Percent_Identity=23.9463601532567, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 73108; Mature: 73108

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVLCEKPSQGRDIAKFLGATQRGEGFLNGPGVTVTWARGHLLETAEPEVYGEQYGKPW
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
RTDVLPLLPQQWKLVVKADAKAQFAVINRLLKQVDEVVIATDADREGEVIARELLDYCKF
CCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHH
QGRVFRLWMSALDDASIRSALADLWPSSRTESLYYAGVGRSRADWLIGMNLTRLFTLLGR
CCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHC
EAGIGNVLSVGRVQTPTLAIVVKRDREIDNFVSKPYFEVIATMAVNGVLFPSKWVPAAQY
CCCCCCHHHCCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHCCEECCCCCCCHHHH
CDEEKRCIQSGVAAQVVQLCRQTPTAAIINCQIERKKQSAPLAFSLSTLQQACSRHWGMP
CCHHHHHHHHCHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCEEEEHHHHHHHHHHHCCCC
AQTVLDIAQKLYETHKLTTYPRTDCGYLPLSMREEIPQVLSAVVGTDPALQPVVASLDTQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVSRIWNDKKITAHHGIIPTKHTGDLSKLSDAERNVYQLVRLHYLAQFLPQMEIDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
FNIGGQLFRTRGNVLVKPGWKSLFGKMSEDDEDNTGETTLPAMQAGQVCQVQGAEQKVLQ
HHCCCHHEEECCCEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCEEEECCCHHHHHH
TKPPAPYNDGTLIAAMTNAAAFVTDAALKKVLKENAGIGTEATRAGIIDTLLKRGFLVRE
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHH
KKTLKSTPLGRNLVDVLPGTLTNPGLTALWEQMLDEVAAGRVSLDDFMAKQSQWVSQLVA
HHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QGRSQPLTMQAPPSPPCPVCGGKTLQRNGKSGLFWGCLKYPECKGIVNNGNKGPRGRRRK
CCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PSPA
CCCC
>Mature Secondary Structure
MRLVLCEKPSQGRDIAKFLGATQRGEGFLNGPGVTVTWARGHLLETAEPEVYGEQYGKPW
CEEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCC
RTDVLPLLPQQWKLVVKADAKAQFAVINRLLKQVDEVVIATDADREGEVIARELLDYCKF
CCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHH
QGRVFRLWMSALDDASIRSALADLWPSSRTESLYYAGVGRSRADWLIGMNLTRLFTLLGR
CCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHC
EAGIGNVLSVGRVQTPTLAIVVKRDREIDNFVSKPYFEVIATMAVNGVLFPSKWVPAAQY
CCCCCCHHHCCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHCCEECCCCCCCHHHH
CDEEKRCIQSGVAAQVVQLCRQTPTAAIINCQIERKKQSAPLAFSLSTLQQACSRHWGMP
CCHHHHHHHHCHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCEEEEHHHHHHHHHHHCCCC
AQTVLDIAQKLYETHKLTTYPRTDCGYLPLSMREEIPQVLSAVVGTDPALQPVVASLDTQ
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FVSRIWNDKKITAHHGIIPTKHTGDLSKLSDAERNVYQLVRLHYLAQFLPQMEIDVTEAT
HHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHH
FNIGGQLFRTRGNVLVKPGWKSLFGKMSEDDEDNTGETTLPAMQAGQVCQVQGAEQKVLQ
HHCCCHHEEECCCEEECCCHHHHHCCCCCCCCCCCCCCCCCHHHCCCEEEECCCHHHHHH
TKPPAPYNDGTLIAAMTNAAAFVTDAALKKVLKENAGIGTEATRAGIIDTLLKRGFLVRE
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHH
KKTLKSTPLGRNLVDVLPGTLTNPGLTALWEQMLDEVAAGRVSLDDFMAKQSQWVSQLVA
HHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
QGRSQPLTMQAPPSPPCPVCGGKTLQRNGKSGLFWGCLKYPECKGIVNNGNKGPRGRRRK
CCCCCCEEEECCCCCCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
PSPA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]