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The map label for this gene is dcuB [H]

Identifier: 29144470

GI number: 29144470

Start: 4376874

End: 4378214

Strand: Reverse

Name: dcuB [H]

Synonym: t4208

Alternate gene names: 29144470

Gene position: 4378214-4376874 (Counterclockwise)

Preceding gene: 29144471

Following gene: 29144469

Centisome position: 91.37

GC content: 53.77

Gene sequence:

>1341_bases
ATGTTATTTAGTATACAGCTTCTCATAATATTAATATGTCTGTTTTATGGTGCCCGAAAGGGCGGGATCGCGCTCGGGTT
GTTGGGCGGTATCGGTCTGGTCATTCTGGTGTTTGTTTTCCATCTCCAGCCAGGTAAACCGCCCGTTGACGTAATGCTGG
TCATTATCGCGGTAGTCGCCGCGTCGGCGACGTTGCAGGCGTCAGGCGGGCTGGATGTGATGTTGCAGATTGCCGAAAAG
CTGCTGCGCCGCAACCCCAAATACGTCTCTATTGTGGCGCCGTTCGTCACCTGTACCCTGACGATTCTGTGTGGGACAGG
CCACGTGGTTTACACCATTTTGCCGATTATCTATGACGTGGCGATCAAGAATAATATCCGTCCGGAACGTCCAATGGCGG
CCAGTTCTATCGGCGCGCAAATGGGTATCATCGCCAGTCCGGTTTCCGTCGCCGTGGTTTCTCTGGTAGCGATGCTGGGC
AACGTGACATTTGACGGAAAACATCTGGAGTTCCTCGATCTGCTGTCGATCACCATCCCGTCTACCCTGCTCGGTATCCT
GGCAATCGGTATTTTTAGTTGGTTCCGCGGTAAAGATCTGGATAAAGACGAAGCGTTTCAGAAATTTATCTCCGTACCGG
AAAACCGTCAGTACGTGTACGGCGATACCGCGACGCTGCTGGATAAAAAACTGCCGAAAAGCAACTGGCTGGCGATGTGG
ATCTTCCTGGCGGCGATTGCCGTGGTCGCTCTCCTGGGCGCGGACTCCGACTTACGTCCAACCTTCGGCGGCAAACCGTT
GTTGATGGTGCTGGTCATTCAGATGTTCATGCTGCTGACCGGGGCACTCATTATCATTCTGACCAAAACCAATCCTGCGT
CTATCTCAAAAAACGAAGTTTTTCGTTCCGGTATGATTGCGATTGTCGCGGTATACGGGATCGCCTGGATGGCGGAAACC
ATGTTCGGCGCGCATATGTCGGAAATTCAGGGCGTGCTGGGCGAAATGGTCAAAGAGTATCCGTGGGCCTACGCCATCGT
TCTGCTGCTGGTCTCCAAGTTTGTTAACTCCCAAGCAGCGGCGCTGGCGGCGATTGTTCCCGTCGCGCTGGCTATCGGTG
TCGATCCGGCGTATATCGTGGCCTCTGCGCCGGCATGTTATGGCTACTATATCCTGCCGACCTACCCAAGCGATCTGGCG
GCGATTCAGTTTGACCGTTCCGGCACAACCCGTATTGGTCGCTTCGTCATTAACCACAGCTTCATTCTGCCGGGTTTGAT
TGGCGTGAGCGTCTCCTGCGTCTTTGGCTGGATCTTTGCCGCAATGTACGGATTCCTGTAA

Upstream 100 bases:

>100_bases
TTGAATATTCGCCATTTCCTGATGCTCATTCACACAAATCAGAAGATGGCTGTGATCCATTTATCAAAATTGACTGGGTT
TATCGCGAGGATAAATAAAA

Downstream 100 bases:

>100_bases
TGGCCGCGCGCGCCGCCCGGCTATGCGCGGCGCGCGACTTAGAGCCTGGCCCATGAGGCTCTTAATACATTTTCTTACTA
TTAGGCTGGAAGCACATGTC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MLFSIQLLIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGKHLEFLDLLSITIPSTLLGILAIGIFSWFRGKDLDKDEAFQKFISVPENRQYVYGDTATLLDKKLPKSNWLAMW
IFLAAIAVVALLGADSDLRPTFGGKPLLMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTRIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL

Sequences:

>Translated_446_residues
MLFSIQLLIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGKHLEFLDLLSITIPSTLLGILAIGIFSWFRGKDLDKDEAFQKFISVPENRQYVYGDTATLLDKKLPKSNWLAMW
IFLAAIAVVALLGADSDLRPTFGGKPLLMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTRIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL
>Mature_446_residues
MLFSIQLLIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEK
LLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
NVTFDGKHLEFLDLLSITIPSTLLGILAIGIFSWFRGKDLDKDEAFQKFISVPENRQYVYGDTATLLDKKLPKSNWLAMW
IFLAAIAVVALLGADSDLRPTFGGKPLLMVLVIQMFMLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAET
MFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLA
AIQFDRSGTTRIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=446, Percent_Identity=97.5336322869955, Blast_Score=791, Evalue=0.0,
Organism=Escherichia coli, GI1790580, Length=441, Percent_Identity=36.734693877551, Blast_Score=267, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 47924; Mature: 47924

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFSIQLLIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGKHLEFLDLLSITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
STLLGILAIGIFSWFRGKDLDKDEAFQKFISVPENRQYVYGDTATLLDKKLPKSNWLAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCHHHHH
IFLAAIAVVALLGADSDLRPTFGGKPLLMVLVIQMFMLLTGALIIILTKTNPASISKNEV
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTRIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVSVSCVFGWIFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLFSIQLLIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQPGKPPVDVMLVIIAVVA
CEEHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTILCGTGHVVYTILPIIYDV
HHHHEECCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLGNVTFDGKHLEFLDLLSITIP
HHHCCCCCCCCCHHHHHCCHHCHHHCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
STLLGILAIGIFSWFRGKDLDKDEAFQKFISVPENRQYVYGDTATLLDKKLPKSNWLAMW
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHCCCCCCHHHHH
IFLAAIAVVALLGADSDLRPTFGGKPLLMVLVIQMFMLLTGALIIILTKTNPASISKNEV
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHH
FRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLGEMVKEYPWAYAIVLLLVSKFVNSQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHH
ALAAIVPVALAIGVDPAYIVASAPACYGYYILPTYPSDLAAIQFDRSGTTRIGRFVINHS
HHHHHHHHHHHHCCCCHHHEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHHHHHCC
FILPGLIGVSVSCVFGWIFAAMYGFL
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]