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The map label for this gene is yjbN

Identifier: 29144411

GI number: 29144411

Start: 4293734

End: 4294732

Strand: Direct

Name: yjbN

Synonym: t4149

Alternate gene names: 29144411

Gene position: 4293734-4294732 (Clockwise)

Preceding gene: 29144410

Following gene: 29144412

Centisome position: 89.6

GC content: 54.65

Gene sequence:

>999_bases
ATGCAGCCTGAAACCCAGTCCTCCGCTTTACCTGCTTATCGCTTCTCCATCGCGCCTATGCTCGACTGGACGGACAGACA
TTGCCGCTATTTCCTGCGTTTGCTGTCTCGCCAGACGCTGCTCTACACCGAAATGGTGACCACGGGCGCGATTATTCACG
GTAAGGGTGACTATCTGGCTTATAGCGAAGAAGAGCATCCGGTCGCTCTACAGCTTGGTGGAAGCGATCCGGCTCAGCTT
GCGCATTGTGCAAAGCTGGCGGAAGCGCGTGGCTACGATGAAATTAACCTCAACGTGGGGTGCCCTTCCGATCGCGTGCA
AAATGGTATGTTTGGCGCCTGTTTGATGGGCAATGCGCAACTGGTCGCCGATTGTGTTAAAGCCATGCGTGATGTCGTCT
CGATTCCGGTGACGGTAAAAACCCGCATTGGTATTGACGATCAGGACAGTTATGCGTTCCTGTGTGATTTCATTAATACG
GTTTCCGGTCAGGGCGAATGCGAGATGTTTATTATCCATGCGCGCAAAGCCTGGCTTTCTGGCTTAAGCCCGAAAGAAAA
TCGTGAGATCCCGCCGCTGGATTACCCGCGCGTCTATCAGCTAAAGCGGGATTTTCCGCACCTGACCATGTCCATTAACG
GCGGCATCAAATCGTTGGAAGAGGCGAAGGAACACTTGCGCCATATGGATGGCGTCATGGTTGGCCGCGAAGCTTATCAG
AATCCGGGTATACTGGCCGCGGTGGATCGGGAGATCTTTGGCGCCGATACCACCGATACCGACCCGGTTGCGGTGGTTCG
CGCGATGTATCCCTATATTGAGCGTGAATTGAGCCAGGGAGCGTATCTGGGGCATATCACTCGCCATATGCTGGGGTTGT
TCCAGGGTATCCCCGGCGCGCGACAGTGGCGTCGCTATCTGAGCGAAAACGCCCATAAAGCTGGCGCGGATGCTGCTGTA
CTGGAGCAGGCGCTAAAACTGATAGCAGACAAGCGTTAA

Upstream 100 bases:

>100_bases
AATGACATTCTGAGGTGCTTTCACCGGAGGCGCTACGCCTCCGGTTTAGCGTTATGCTATAGTAACGCCCCTTTTTTAAC
CGGATGCTTATTTATTCGCC

Downstream 100 bases:

>100_bases
AAGTTCGCCAAAAATTAGTCAATCTCACCACGCTCTGCGCAGTTTTGCAGGGCGTTTTCTTTATATATCAAAAACATAAA
TATTGGCATGATTTTTGTAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDTDPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADAAV
LEQALKLIADKR

Sequences:

>Translated_332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDTDPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADAAV
LEQALKLIADKR
>Mature_332_residues
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAQL
AHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINT
VSGQGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
NPGILAAVDREIFGADTTDTDPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADAAV
LEQALKLIADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=23.3766233766234, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=319, Percent_Identity=94.6708463949843, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=24.7011952191235, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.530612244898, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24580595, Length=313, Percent_Identity=23.3226837060703, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920448, Length=313, Percent_Identity=23.3226837060703, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_SALTI (Q8Z1T1)

Other databases:

- EMBL:   AL627282
- EMBL:   AE014613
- RefSeq:   NP_458541.1
- RefSeq:   NP_807753.1
- ProteinModelPortal:   Q8Z1T1
- SMR:   Q8Z1T1
- GeneID:   1069757
- GeneID:   1250656
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t4149
- KEGG:   sty:STY4439
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- BioCyc:   SENT209261:T4149-MONOMER
- BioCyc:   SENT220341:STY4439-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 36987; Mature: 36987

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLA
CCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQ
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEECCCHH
LVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINTVSGQGECEMFIIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHC
GLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHC
NPGILAAVDREIFGADTTDTDPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGA
CCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
RQWRRYLSENAHKAGADAAVLEQALKLIADKR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPETQSSALPAYRFSIAPMLDWTDRHCRYFLRLLSRQTLLYTEMVTTGAIIHGKGDYLA
CCCCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEE
YSEEEHPVALQLGGSDPAQLAHCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQ
ECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEECCCHH
LVADCVKAMRDVVSIPVTVKTRIGIDDQDSYAFLCDFINTVSGQGECEMFIIHARKAWLS
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEHHHHHC
GLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKEHLRHMDGVMVGREAYQ
CCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHC
NPGILAAVDREIFGADTTDTDPVAVVRAMYPYIERELSQGAYLGHITRHMLGLFQGIPGA
CCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
RQWRRYLSENAHKAGADAAVLEQALKLIADKR
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504