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The map label for this gene is dgkA [H]

Identifier: 29144404

GI number: 29144404

Start: 4288544

End: 4288912

Strand: Direct

Name: dgkA [H]

Synonym: t4142

Alternate gene names: 29144404

Gene position: 4288544-4288912 (Clockwise)

Preceding gene: 29144402

Following gene: 29144405

Centisome position: 89.49

GC content: 50.14

Gene sequence:

>369_bases
ATGGCCAATAATACCACTGGATTCACCAGAATTATCAAAGCGGCAGGATATTCCTGGAAAGGATTTCGCGCCGCATGGAC
TCACGAAGCGGCGTTTCGCCAGGAAAGCCTCGCGGTATTGCTGGGCGTCATCATCGCCTGCTGGCTGGATGTGGATGCGA
TTACCCGTGTGTTATTGATAGGTTCCGTTTTACTTATCATGATTGTTGAAATCCTCAACAGCGCTATTGAAGCGGTCGTT
GACCGCATCGGTTCTGAATACCATGAGCTTTCCGGCCGTGCGAAAGATATGGGATCGGCTGCGGTGCTACTGTCTATTTT
CGTTGCCCTGATGACCTGGGGCATCCTGTTATGGTCACATTTTCGATAA

Upstream 100 bases:

>100_bases
GTTGTATCCCGTCATACTTCGCGCTGCGTTACGGCAGTCCGAATCGTGCAGGGCAACCGTATAATGTCATTGACGTTAAT
AATGTAAAAAGGTTCTTTTG

Downstream 100 bases:

>100_bases
AGGTTCCAAATTTTATAAATCTCTTGTTTTTTGCGCCGTTTGTGGTTCCAAAATCACCTTTAACTGTATATACTCACAGC
ATGACTGTATATACACCCAG

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAGK; Diglyceride kinase; DGK [H]

Number of amino acids: Translated: 122; Mature: 121

Protein sequence:

>122_residues
MANNTTGFTRIIKAAGYSWKGFRAAWTHEAAFRQESLAVLLGVIIACWLDVDAITRVLLIGSVLLIMIVEILNSAIEAVV
DRIGSEYHELSGRAKDMGSAAVLLSIFVALMTWGILLWSHFR

Sequences:

>Translated_122_residues
MANNTTGFTRIIKAAGYSWKGFRAAWTHEAAFRQESLAVLLGVIIACWLDVDAITRVLLIGSVLLIMIVEILNSAIEAVV
DRIGSEYHELSGRAKDMGSAAVLLSIFVALMTWGILLWSHFR
>Mature_121_residues
ANNTTGFTRIIKAAGYSWKGFRAAWTHEAAFRQESLAVLLGVIIACWLDVDAITRVLLIGSVLLIMIVEILNSAIEAVVD
RIGSEYHELSGRAKDMGSAAVLLSIFVALMTWGILLWSHFR

Specific function: Recycling of diacylglycerol produced during the turnover of membrane phospholipid [H]

COG id: COG0818

COG function: function code M; Diacylglycerol kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial diacylglycerol kinase family [H]

Homologues:

Organism=Escherichia coli, GI1790475, Length=121, Percent_Identity=86.7768595041322, Blast_Score=213, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000829 [H]

Pfam domain/function: PF01219 DAGK_prokar [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 13435; Mature: 13304

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS01069 DAGK_PROKAR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANNTTGFTRIIKAAGYSWKGFRAAWTHEAAFRQESLAVLLGVIIACWLDVDAITRVLLI
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVLLIMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLLSIFVALMTWGILLWSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FR
CC
>Mature Secondary Structure 
ANNTTGFTRIIKAAGYSWKGFRAAWTHEAAFRQESLAVLLGVIIACWLDVDAITRVLLI
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVLLIMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAVLLSIFVALMTWGILLWSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]