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The map label for this gene is aceK

Identifier: 29144375

GI number: 29144375

Start: 4252425

End: 4254206

Strand: Direct

Name: aceK

Synonym: t4113

Alternate gene names: 29144375

Gene position: 4252425-4254206 (Clockwise)

Preceding gene: 29144374

Following gene: 29144377

Centisome position: 88.74

GC content: 53.09

Gene sequence:

>1782_bases
ATGCCGCGTGGCCTGGAATTACTGATTGCTCAAACCATCCTGCAAGGCTTTGACGCGCAGTATGGTCGATTTCTGGAAGT
GACTTCCGGCGCGCAGCAGCGTTTTGAACAGGCCGACTGGCACGCCGTCCAGCAGGCGATGAAAAGCCGTATCCACCTCT
ATGATCATCATGTGGGTCTGGTGGTGGAGCAACTGCGCTGTATTACCGATGGCAAAAGTACCGACGCGGATTTCCTTTTA
CGCGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTCAACTCCGTTTA
TTGCCGGTTATTTGACCACCGCTCGTTAACACCCGAGCGGTTGTTTATTTTCAGTTCGCAACCGGAGCGTCGCTTCCGTA
CTATCCCGCGCCCGCTGGCCAAAGACTTCTTTCCCGATCACGGTTGGGAGCCGCTGTTGATGCGCATACTCAGTGACTTA
CCGCTGTGTCTACCCTGGCAGAATAAAAGCCGGGATATCCGCTATATTATCGCGCACCTGACTGAAACACTGGGCGAGGA
CGCGCTACCTCGCTGCCATGTCCAGGTGGCGAACGAGTTGTTTTACCGTAATAAAGCGGCCTGGCTGGTAGGTAAATTAA
CCACACCTGACGGTACGCTGCCGTTTTTATTGCCGATTCATCGCACTGATGAGGGGGAACTGTTTGTCGATACCTGCCTG
ACCACCACGGCAGAAGCGAGCATTGTGTTTGGTTTTGCCCGCTCTTATTTTATGGTGTATGCGCCGCTGCCTGCCGCGCT
GGTTGAGTGGTTGCGTGAAATCCTGCCGGGTAAAACCACTGCCGAACTGTATATGGCGATTGGCTGCCAGAAACATGCGA
AAACCGAAAGCTACCGCGAATATTTATGTTATCTGGCTGAAAGCGATGAGAAATTCATTGAGGCGCCTGGAATCCGCGGC
ATGGTGATGCTGGTATTTACCCTGCCAGGCTTCGACCGGGTATTTAAAATCATTAAAGACAAATTCGCCCCGCAGAAAGA
GATGTCTGCCGCCCATGTGCGTGCCTGTTATCAACTGGTAAAAGAGCATGATCGCGTCGGTCGTATGGCGGATACCCAGG
AGTTTGAGAATTTCGTACTGGATAAACGGCAAATCGATCCGGCGCTGATGGCGCTGTTACGACAAGAAGCGCCAGAGAAG
ATTACCGATCTTGGGGAACATATCGTCATTCGCCATCTCTATATTGAACGTCGCATGGTGCCGCTCAATATCTGGCTGGA
ACAGGTAGAAGGCCAGCAGTTGCGCGATGCGATAGAAGAATATGGTAATGCGATTCGCCAGCTTGCCGCCGCTAATATTT
TCCCCGGCGATATGTTGTTTAAAAACTTTGGCGTTACCCGTCATGGTCGCGTGGTGTTCTACGACTACGATGAAATTTGC
TACATGACGGAAGTGAATTTCCGCGATATTCCGCCAGCGCGTTATCCGGAAGACGAACTGGCCAGCGAACCCTGGTATAG
CGTCTCGCCGGGTGATGTTTTCCCGGAGGAGTTTCGCCACTGGCTGTGTGCCGATCCGCGTATCGGGCCATTGTTTGAAG
AGATGCATGCCGACCTGTTTCGCGCCGATTACTGGCGCGCGCTACAGACGCGTATTAAAGAAGGGCATGTAGAAGATGTG
TATGCCTATCGTCGCCGCCAGCGCTTTAGCGTGCGTTATGAAACCGACCGTAGGCCGGATAAGGCCTTTGCGCCGCCATC
CGGCAATGTACGCCGTGCCTGA

Upstream 100 bases:

>100_bases
TTGATGCCCGGTGGCGCTGCGCTTACCGGGCCTACAGATAGCGTAGGCCGGATAAGCGAACGCGCCATCCGGCATGATGT
GGTGCCGGATGAGGGAAAAA

Downstream 100 bases:

>100_bases
TGGCGCTGCGCTTATCAGGCCTACAAATCAGTTCCAACCCTTGCTTATCTAAACCCACCATAGGCCAGCGTCACCTCTTT
CGCCGCTTTAATCACCATGG

Product: bifunctional isocitrate dehydrogenase kinase/phosphatase protein

Products: NA

Alternate protein names: IDH kinase/phosphatase; IDHK/P

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITDGKSTDADFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDL
PLCLPWQNKSRDIRYIIAHLTETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLCYLAESDEKFIEAPGIRG
MVMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEK
ITDLGEHIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQTRIKEGHVEDV
YAYRRRQRFSVRYETDRRPDKAFAPPSGNVRRA

Sequences:

>Translated_593_residues
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITDGKSTDADFLL
RVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDL
PLCLPWQNKSRDIRYIIAHLTETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCL
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLCYLAESDEKFIEAPGIRG
MVMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEK
ITDLGEHIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
YMTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQTRIKEGHVEDV
YAYRRRQRFSVRYETDRRPDKAFAPPSGNVRRA
>Mature_592_residues
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGLVVEQLRCITDGKSTDADFLLR
VKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDLP
LCLPWQNKSRDIRYIIAHLTETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCLT
TTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYREYLCYLAESDEKFIEAPGIRGM
VMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEKI
TDLGEHIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICY
MTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQTRIKEGHVEDVY
AYRRRQRFSVRYETDRRPDKAFAPPSGNVRRA

Specific function: Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source

COG id: COG4579

COG function: function code T; Isocitrate dehydrogenase kinase/phosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AceK family

Homologues:

Organism=Escherichia coli, GI1790446, Length=573, Percent_Identity=92.8446771378709, Blast_Score=1105, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ACEK_SALTI (Q8Z1V8)

Other databases:

- EMBL:   AL627282
- EMBL:   AE014613
- RefSeq:   NP_458505.1
- RefSeq:   NP_807717.1
- GeneID:   1069836
- GeneID:   1250620
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t4113
- KEGG:   sty:STY4403
- HOGENOM:   HBG298593
- OMA:   RMTPLNI
- ProtClustDB:   PRK02946
- BioCyc:   SENT209261:T4113-MONOMER
- BioCyc:   SENT220341:STY4403-MONOMER
- BRENDA:   2.7.11.5
- GO:   GO:0005737
- HAMAP:   MF_00747
- InterPro:   IPR010452
- PIRSF:   PIRSF000719
- ProDom:   PD043552

Pfam domain/function: PF06315 AceK

EC number: =2.7.11.5

Molecular weight: Translated: 69250; Mature: 69119

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: ACT_SITE 371-371 BINDING 336-336

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGL
CCCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEQLRCITDGKSTDADFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDLPLCLPWQNKSRDIRYIIAHL
EEEECCCCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
TETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCL
HHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLCYLAESDEKFIEAPGIRGMVMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEKITDLGEHIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQTRIKEGHVEDVYAYRRRQRFSVRYETDRRPDKAFAPPSGNVRRA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQQAMKSRIHLYDHHVGL
CCCHHHHHHHHHHHHCCHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEQLRCITDGKSTDADFLLRVKEHYTRLLPDYPRFEIAESFFNSVYCRLFDHRSLTPER
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCE
LFIFSSQPERRFRTIPRPLAKDFFPDHGWEPLLMRILSDLPLCLPWQNKSRDIRYIIAHL
EEEECCCCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHH
TETLGEDALPRCHVQVANELFYRNKAAWLVGKLTTPDGTLPFLLPIHRTDEGELFVDTCL
HHHHCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEHHHH
TTTAEASIVFGFARSYFMVYAPLPAALVEWLREILPGKTTAELYMAIGCQKHAKTESYRE
HHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHH
YLCYLAESDEKFIEAPGIRGMVMLVFTLPGFDRVFKIIKDKFAPQKEMSAAHVRACYQLV
HHHHHHCCCHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KEHDRVGRMADTQEFENFVLDKRQIDPALMALLRQEAPEKITDLGEHIVIRHLYIERRMV
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
PLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEIC
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEECCCEE
YMTEVNFRDIPPARYPEDELASEPWYSVSPGDVFPEEFRHWLCADPRIGPLFEEMHADLF
EEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHH
RADYWRALQTRIKEGHVEDVYAYRRRQRFSVRYETDRRPDKAFAPPSGNVRRA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504