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The map label for this gene is yheL

Identifier: 29144321

GI number: 29144321

Start: 4211028

End: 4211315

Strand: Direct

Name: yheL

Synonym: t4056

Alternate gene names: 29144321

Gene position: 4211028-4211315 (Clockwise)

Preceding gene: 29144320

Following gene: 29144322

Centisome position: 87.88

GC content: 52.78

Gene sequence:

>288_bases
ATGCTGCATACCTTACCTCATTGCGCTTCGGGCGTTGATTTTCCCGCTTTGCTGCGCCTGCTAAAAGAGGGCGATGCGCT
TTTGCTGCTCCAGGATGGCGTTACCGTCGCGATTGAAGGTAACCGCTTCCTTGAAAGTCTGCGCGATGCCCCCATAACGG
TCTATGCCTTGAAAGAAGACATTGACGCTCGCGGCCTGGGTGGTCAAATTTCAGACAGTGTCGTCAGGGTTGACTATACT
GAGTTTGTCAGACTCACGGTTAAGTATGCCAACCAGATGGCCTGGTGA

Upstream 100 bases:

>100_bases
GAAAACGTTAATTTTGTGGTAAACGCCACGCCGCTGGAACCCGTGGCGTTACGCCGCGAGCTTGGCAACTATGATGTCAT
CCTGAGATTCTGAGGCGTTT

Downstream 100 bases:

>100_bases
TGGCGGGATCGTTGTATATTTCTTGACACCTTTTCGACACCGCCCTAAAATTCGGCGTCCTCATATTGTGTGAGGGCGTT
TTATTACGTGTTTACGAAGC

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLHTLPHCASGVDFPALLRLLKEGDALLLLQDGVTVAIEGNRFLESLRDAPITVYALKEDIDARGLGGQISDSVVRVDYT
EFVRLTVKYANQMAW

Sequences:

>Translated_95_residues
MLHTLPHCASGVDFPALLRLLKEGDALLLLQDGVTVAIEGNRFLESLRDAPITVYALKEDIDARGLGGQISDSVVRVDYT
EFVRLTVKYANQMAW
>Mature_95_residues
MLHTLPHCASGVDFPALLRLLKEGDALLLLQDGVTVAIEGNRFLESLRDAPITVYALKEDIDARGLGGQISDSVVRVDYT
EFVRLTVKYANQMAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=68.4210526315789, Blast_Score=134, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_SALA4 (B5F8G1)

Other databases:

- EMBL:   CP001138
- RefSeq:   YP_002148368.1
- ProteinModelPortal:   B5F8G1
- SMR:   B5F8G1
- GeneID:   6796624
- GenomeReviews:   CP001138_GR
- KEGG:   sea:SeAg_B3646
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- ProtClustDB:   PRK13510
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10502; Mature: 10502

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTLPHCASGVDFPALLRLLKEGDALLLLQDGVTVAIEGNRFLESLRDAPITVYALKED
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCEEEEEECHHHHHHHCCCCEEEEEECCC
IDARGLGGQISDSVVRVDYTEFVRLTVKYANQMAW
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLHTLPHCASGVDFPALLRLLKEGDALLLLQDGVTVAIEGNRFLESLRDAPITVYALKED
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCEEEEEECHHHHHHHCCCCEEEEEECCC
IDARGLGGQISDSVVRVDYTEFVRLTVKYANQMAW
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA