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The map label for this gene is gntR [H]

Identifier: 29144246

GI number: 29144246

Start: 4117396

End: 4118391

Strand: Direct

Name: gntR [H]

Synonym: t3978

Alternate gene names: 29144246

Gene position: 4117396-4118391 (Clockwise)

Preceding gene: 29144245

Following gene: 29144247

Centisome position: 85.92

GC content: 57.53

Gene sequence:

>996_bases
ATGAAAAAGAAAAGACCCGTACTTCAGGATGTAGCCGACCGCGTGGGCGTGACCAAAATGACGGTCAGCCGCTTTTTGCG
TAACCCGGAGCAGGTCTCCGTGGCGCTGCGGGGCAAAATTGCCGCTGCACTTGATGAGCTGGGTTATATTCCCAATCGCG
CTCCTGATATCCTTTCTAACGCCACCAGTCGCGCTATCGGCGTGCTGTTACCGTCTCTCACCAACCAGGTCTTTGCGGAA
GTGTTACGCGGCATTGAGGCCGTCACCGATGCCCACGGGTATCAGACCATGCTGGCGCACTACGGCTATAAACCGGAGAT
GGAGCAGGAGCGCCTGGAATCGATGCTCTCCTGGAATATCGACGGTCTTATCCTCACAGAACGTACCCATACGCCGCGCA
CCTTAAAAATGATCGAAGTCGCCGGGATTCCGGTGGTGGAACTGATGGACAGCCAGTCGCCGTGTCTCGATATTGCCGTT
GGTTTTGACAACTTCGAGGCCGCCCGTCAGATGACCGCCGCGATTATCGCGCGTGGTCATCGTCATATCGCCTATCTGGG
GGCGCGCCTCGACGAACGTACTATCATCAAGCAGAAGGGCTATGAACAGGCGATGCGGGACGCCGGCCTGGTTCCTTACA
GTGTGATGATGGAGCAATCTTCATCCTACTCTTCCGGTATCGAACTCATGCGCCAGGCGCGACGCGAATACCCACAGCTT
GACGGTATTTTTTGCACCAACGATGACCTGGCGGTGGGGGCGGCCTTCGAATGCCAGCGCCTGGGGCTAAAAATCCCGGA
CGACATGGCGATCGCCGGGTTCCACGGTCATGACATCGGCCAGGTGATGGAACCGCGTCTGGCAAGCGTCCTGACGCCGC
GCGAGCGAATGGGCAGCATTGGCGCGGAACGTCTGTTGGCCCGCATTCGCGGCGAAACGGTCACGCCGAAAATGTTAGAT
TTAGGTTTCACCTTGTCACCGGGCGGATCTATTTAG

Upstream 100 bases:

>100_bases
TTGTGTATTTCTCATAATAAAAACTCCTTTTTCTCTGCGAAGGTTCACGCACGTCCGTGGTAAACTGGGGAAATCTATCC
CTTTTTAACGCTTCAGGACG

Downstream 100 bases:

>100_bases
TCTGACAACTTTGAAGTAGCTCACACTTATTCACTTTTGGCACGAATGGTCATTGCGTCGTGAATGGTGCGGTTGGACAA
TGTTACCGATAACAGTTACC

Product: gluconate utilization operon repressor

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIEAVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMMEQSSSYSSGIELMRQARREYPQL
DGIFCTNDDLAVGAAFECQRLGLKIPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGETVTPKMLD
LGFTLSPGGSI

Sequences:

>Translated_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIEAVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMMEQSSSYSSGIELMRQARREYPQL
DGIFCTNDDLAVGAAFECQRLGLKIPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGETVTPKMLD
LGFTLSPGGSI
>Mature_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIEAVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
GFDNFEAARQMTAAIIARGHRHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMMEQSSSYSSGIELMRQARREYPQL
DGIFCTNDDLAVGAAFECQRLGLKIPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGETVTPKMLD
LGFTLSPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=97.2809667673716, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=45.7831325301205, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790369, Length=323, Percent_Identity=29.4117647058824, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=28.3536585365854, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=26.9592476489028, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=26.6666666666667, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788474, Length=306, Percent_Identity=25.8169934640523, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787580, Length=323, Percent_Identity=26.0061919504644, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=25.3125, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=325, Percent_Identity=25.8461538461538, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=21.8430034129693, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790689, Length=338, Percent_Identity=22.189349112426, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1786268, Length=335, Percent_Identity=24.4776119402985, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36512; Mature: 36512

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIEAVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTAAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMMEQSSSYSSGIELMRQARREYPQL
HHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DGIFCTNDDLAVGAAFECQRLGLKIPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGETVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC
>Mature Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIEAVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAVGFDNFEAARQMTAAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMRDAGLVPYSVMMEQSSSYSSGIELMRQARREYPQL
HHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCC
DGIFCTNDDLAVGAAFECQRLGLKIPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGETVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]