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The map label for this gene is php [H]

Identifier: 29144239

GI number: 29144239

Start: 4109906

End: 4110940

Strand: Reverse

Name: php [H]

Synonym: t3971

Alternate gene names: 29144239

Gene position: 4110940-4109906 (Counterclockwise)

Preceding gene: 29144240

Following gene: 29144237

Centisome position: 85.79

GC content: 50.63

Gene sequence:

>1035_bases
ATGAAAGGTTTCCTGCAAACCGTAACAGGCCCGGTGGCGCATACGGATATGGGGCTGACGCTACCGCATGAACATCTGTT
TAACGATCTGTCTTCGGTCGTAGACGAGCCACATTATGCTTTTTCACAGCAACTGGTGGGAAAGAAAGTCAGTGCTGACC
TGCAGTGGGGGCTGAAACACGACCCATACTGCTGCGCCGATAACATGGACAGAAAAGAGATTGATGATGTTATCTTCGAA
ATCAATAACTTTATGTCTTTGGGCGGCAGGACGATTGTCGACGCCACCGGTTCAGAATCCATTGGACGGGATGCCTCAGC
GCTACGAGAAGTTGCCTTAAAAACGGGTTTGAATATCGTTGCGTCATCCGGGCCTTATCTGGAGAAATTTGAAAGCACCC
GGATCCATAAACCTGTTGAGCTGTTAGCCTCACTCATTGATAAAGAACTGAATCAGGGTATTGGCGAGACGGATATTCGC
GCTGGCATGATCGGTGAAATCGGCGTATCTCCCGCGTTTACCCAAGCGGAGCGTAACAGCCTGCGGGCAGCGTCTCTGGC
ACAATGTAACAACCCCCACACGGCGATGAATATCCATATGCCAGGGTGGCTGCGACTGGGTGATGAGGTGCTGGATATCG
TGCTGGAAGAAATGAACGTCTCTCCGGCAAAAGTCTCGCTGGCGCATTCGGACCCTTCAGGAAAAGATGTCGTCTATCAA
CGTAAAATGCTCGACCGCGGCGTATGGCTTGAATTTGACATGATTGGTCTGGACATCACCTTTCCAAAAGAAGGTGTCGC
GCCGGGCGTTCAGGAGACCGCAGATGCAGTAGCGAATCTGATTGCACTGGGTTACGCCGACCAAATCGTTTTAAGCCATG
ATGTTTTTCTCAAACAGATGTGGGCGAAAAATGGCGGAAATGGCTGGGGGTTTGTCCCGAATGTTTTCCTCGCCTATTTG
GCTGCTCGCGGAGTAGACAACGATACTCTGCGCAAGCTTTGTATTGATAATCCAGCCCGATTACTCACGGCATAA

Upstream 100 bases:

>100_bases
TCACCATCATCGGTACGCTGTTCGCGTTAGTCGGTGCGATTGTGACCACCATCCCTGAATACAGAGCATCCAAAATGGCG
CAAAAAATGGAGGTGTCAGA

Downstream 100 bases:

>100_bases
TCTGCAAATAGCCCGGTGTCTGACACCGGGCCTGGAATTAAAAATCAGTATCCCGCCGTTAAATCATCCACGGAACGCGG
GTCAGACGCACCGTACAGTT

Product: phophotriesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKGFLQTVTGPVAHTDMGLTLPHEHLFNDLSSVVDEPHYAFSQQLVGKKVSADLQWGLKHDPYCCADNMDRKEIDDVIFE
INNFMSLGGRTIVDATGSESIGRDASALREVALKTGLNIVASSGPYLEKFESTRIHKPVELLASLIDKELNQGIGETDIR
AGMIGEIGVSPAFTQAERNSLRAASLAQCNNPHTAMNIHMPGWLRLGDEVLDIVLEEMNVSPAKVSLAHSDPSGKDVVYQ
RKMLDRGVWLEFDMIGLDITFPKEGVAPGVQETADAVANLIALGYADQIVLSHDVFLKQMWAKNGGNGWGFVPNVFLAYL
AARGVDNDTLRKLCIDNPARLLTA

Sequences:

>Translated_344_residues
MKGFLQTVTGPVAHTDMGLTLPHEHLFNDLSSVVDEPHYAFSQQLVGKKVSADLQWGLKHDPYCCADNMDRKEIDDVIFE
INNFMSLGGRTIVDATGSESIGRDASALREVALKTGLNIVASSGPYLEKFESTRIHKPVELLASLIDKELNQGIGETDIR
AGMIGEIGVSPAFTQAERNSLRAASLAQCNNPHTAMNIHMPGWLRLGDEVLDIVLEEMNVSPAKVSLAHSDPSGKDVVYQ
RKMLDRGVWLEFDMIGLDITFPKEGVAPGVQETADAVANLIALGYADQIVLSHDVFLKQMWAKNGGNGWGFVPNVFLAYL
AARGVDNDTLRKLCIDNPARLLTA
>Mature_344_residues
MKGFLQTVTGPVAHTDMGLTLPHEHLFNDLSSVVDEPHYAFSQQLVGKKVSADLQWGLKHDPYCCADNMDRKEIDDVIFE
INNFMSLGGRTIVDATGSESIGRDASALREVALKTGLNIVASSGPYLEKFESTRIHKPVELLASLIDKELNQGIGETDIR
AGMIGEIGVSPAFTQAERNSLRAASLAQCNNPHTAMNIHMPGWLRLGDEVLDIVLEEMNVSPAKVSLAHSDPSGKDVVYQ
RKMLDRGVWLEFDMIGLDITFPKEGVAPGVQETADAVANLIALGYADQIVLSHDVFLKQMWAKNGGNGWGFVPNVFLAYL
AARGVDNDTLRKLCIDNPARLLTA

Specific function: Its real enzymatic activity is not yet known. It was tested for general esterase, aminopeptidase, sulfatase, phosphatase, carbonic anhydrase, phosphodiesterase, and phosphotriesterase activities with the following substrates:P- nitrophenyl acetate, L-alan

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family [H]

Homologues:

Organism=Homo sapiens, GI20070186, Length=351, Percent_Identity=27.9202279202279, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI47933341, Length=351, Percent_Identity=27.9202279202279, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1789780, Length=331, Percent_Identity=27.4924471299094, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645325, Length=351, Percent_Identity=23.0769230769231, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017947
- InterPro:   IPR001559 [H]

Pfam domain/function: PF02126 PTE [H]

EC number: NA

Molecular weight: Translated: 37540; Mature: 37540

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01322 PHOSPHOTRIESTERASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFLQTVTGPVAHTDMGLTLPHEHLFNDLSSVVDEPHYAFSQQLVGKKVSADLQWGLKH
CCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCC
DPYCCADNMDRKEIDDVIFEINNFMSLGGRTIVDATGSESIGRDASALREVALKTGLNIV
CCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEE
ASSGPYLEKFESTRIHKPVELLASLIDKELNQGIGETDIRAGMIGEIGVSPAFTQAERNS
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHH
LRAASLAQCNNPHTAMNIHMPGWLRLGDEVLDIVLEEMNVSPAKVSLAHSDPSGKDVVYQ
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
RKMLDRGVWLEFDMIGLDITFPKEGVAPGVQETADAVANLIALGYADQIVLSHDVFLKQM
HHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WAKNGGNGWGFVPNVFLAYLAARGVDNDTLRKLCIDNPARLLTA
HHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MKGFLQTVTGPVAHTDMGLTLPHEHLFNDLSSVVDEPHYAFSQQLVGKKVSADLQWGLKH
CCCHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCC
DPYCCADNMDRKEIDDVIFEINNFMSLGGRTIVDATGSESIGRDASALREVALKTGLNIV
CCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCEEE
ASSGPYLEKFESTRIHKPVELLASLIDKELNQGIGETDIRAGMIGEIGVSPAFTQAERNS
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHH
LRAASLAQCNNPHTAMNIHMPGWLRLGDEVLDIVLEEMNVSPAKVSLAHSDPSGKDVVYQ
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
RKMLDRGVWLEFDMIGLDITFPKEGVAPGVQETADAVANLIALGYADQIVLSHDVFLKQM
HHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WAKNGGNGWGFVPNVFLAYLAARGVDNDTLRKLCIDNPARLLTA
HHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9548740 [H]