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The map label for this gene is rsmD [H]

Identifier: 29144219

GI number: 29144219

Start: 4089701

End: 4090297

Strand: Reverse

Name: rsmD [H]

Synonym: t3950

Alternate gene names: 29144219

Gene position: 4090297-4089701 (Counterclockwise)

Preceding gene: 29144224

Following gene: 29144218

Centisome position: 85.36

GC content: 56.45

Gene sequence:

>597_bases
ATGAAAAAACCGACTCACTCTGGCAGCGGCCAAATCCGCATTATTGGCGGGCAATGGCGTGGCCGTAAATTACCGGTTCC
GGACAGCCCGGGTCTGCGTCCTACCACCGATCGCGTGCGCGAAACGTTATTTAACTGGCTGGCTCCGGTAATGGTAGACG
CCCATTGTCTGGATTGCTTTGCCGGCAGCGGCGCGCTGGGTCTGGAAGCGCTATCGCGTTATGCGGCCCAGGCAACGTTA
CTGGAAATGGATCGCGCCGTATCGCAGCAGTTGCAAAAAAATCTGGCGACGCTGAAAGCCAACAATGCTCGCGTCGTCAA
TACCAATACTCTGGCCTTCCTCAGCCAGCCGGGGACGCCGCATCACGTCGTCTTTGTCGACCCGCCGTTTCGTAAAGGAC
TGCTGGAAGAGACGTTACAATTGCTAGAAACCCAGGGATGGCTGGCCGACGACGCGCTAATCTACGTAGAAAGCGAGGTG
GAAAACGGTCTGCCGCCCGTTCCGGCGAACTGGGCATTGTATCGAGAAAAGGTGGCCGGACAGGTCGCTTATCGTCTGTA
TCAGCGCGACGCGCAAGGAGAAAATGATGTTGATTAA

Upstream 100 bases:

>100_bases
ATATACATGGAAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGGAATAACTTGCAAC
AGGCGGTTCGAGCAAGAGAA

Downstream 100 bases:

>100_bases
TCTGGGCCGCTTATTAATGCTCTTCGTGTGGGCATTTTTGATTCTTAATCTGGTGCATCCCTTCCCGCGCCCGCTCAATA
TCTTCGTCAACGTGGCGTTG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEALSRYAAQATL
LEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEV
ENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD

Sequences:

>Translated_198_residues
MKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEALSRYAAQATL
LEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEV
ENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD
>Mature_198_residues
MKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCFAGSGALGLEALSRYAAQATL
LEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTPHHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEV
ENGLPPVPANWALYREKVAGQVAYRLYQRDAQGENDVD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=198, Percent_Identity=86.3636363636364, Blast_Score=362, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21858; Mature: 21858

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCF
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGALGLEALSRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCC
HHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEVENGLPPVPANWALYREKVAG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHH
QVAYRLYQRDAQGENDVD
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKPTHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVMVDAHCLDCF
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGALGLEALSRYAAQATLLEMDRAVSQQLQKNLATLKANNARVVNTNTLAFLSQPGTP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECCCCC
HHVVFVDPPFRKGLLEETLQLLETQGWLADDALIYVESEVENGLPPVPANWALYREKVAG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHCCCCCCCCCHHHHHHHHHH
QVAYRLYQRDAQGENDVD
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]