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The map label for this gene is pitA [H]

Identifier: 29144198

GI number: 29144198

Start: 4064821

End: 4066317

Strand: Reverse

Name: pitA [H]

Synonym: t3927

Alternate gene names: 29144198

Gene position: 4066317-4064821 (Counterclockwise)

Preceding gene: 29144201

Following gene: 29144196

Centisome position: 84.86

GC content: 53.64

Gene sequence:

>1497_bases
ATGCTACATTTGTTTGCTGGCCTGGATTTACATACCGGGCTTTTATTATTGCTTGCTCTGGCTTTTGTGCTGTTCTACGA
AGCGATTAACGGTTTCCATGATACAGCCAATGCAGTCGCAACCGTGATTTACACCCGTGCGATGCGTTCGCAGCTGGCGG
TTGTCATGGCGGCAGTGTTTAACTTTTTTGGCGTTTTGCTGGGGGGTCTCAGCGTGGCGTATGCCATTGTGCATATGCTA
CCGACGGATCTCCTGCTCAACATGGGTTCCGCGCACGGCCTCGCTATGGTCTTTTCCATGTTGCTGGCGGCGATTATCTG
GAACCTCGGTACCTGGTATTTCGGGTTGCCGGCGTCCAGTTCCCACACGCTGATTGGCGCCATTATCGGCATCGGTTTAA
CCAATGCGATGATGACCGGCACGTCAGTGGTGGATGCGCTTAACATCCCGAAAGTTATCAATATTTTCGGTTCACTCATT
ATCTCCCCTATTGTCGGTCTGGTATTCGCCGGCGGTCTGATTTTCTTGCTGCGCCGCTACTGGAGCGGTACTAAGAAACG
CGCCCGTATCCACCTGACGCCAGCCGAACGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCATTCTGGACGCGTATCGCGC
TGATCCTCTCAGCTATCGGCGTGGCGTTTTCGCATGGCGCGAACGATGGTCAGAAAGGCATTGGCCTGGTCATGCTGGTG
CTGATTGGCGTCGCGCCTGCGGGTTTCGTGGTCAATATGAATGCCTCCAGCTACGAAATCACCCGTACGCGCGATGCTAT
CAACAATGTCGAAACGTACTTTGAGCAGCGTCCTGACCTGCTGAAAGCCGTCACCGGCGTTGACCAACTGATTCCCTCTC
CGGAACCGGGCGCGACTGAACCGACTGAATTCCACTGCCATCCGGCTAACACCATTAACGCGCTTAACCGTGCGAAAGGG
ATGCTGGCAAACGTGGAAAGCTACGACAAGTTAAGCGTTGAACAGCGTAGTCAGTTGCGTCGCATTATGCTGTGCATTTC
CGACACGACCGACAAAGTCGTGAAATTGCCTGGCGTAAGCAGCGATGACCAGCGCCTGCTGAAAAAGCTGAAGACCGATA
TGTTAAGCACCATTGAGTATGCGCCGGTGTGGATCATTATGGCAGTGGCGTTGGCGCTGGGTATTGGTACGATGATCGGC
TGGCGTCGCGTGGCGACCACTATCGGCGAGAAAATTGGTAAAAAAGGCATGACGTATGCGCAGGGAATGTCGGCGCAGAT
GACGGCGGCAGTCTCTATCGGTCTTGCCAGCTACACCGGTATGCCAGTTTCTACCACTCACGTACTCTCCTCTTCAGTGG
CGGGAACCATGGTCGTTGATGGCGGCGGGTTACAGCGTAAAACCGTGACCAGCATTCTGATGGCCTGGGTATTTACGTTA
CCGGCGGCGATTATTCTTTCCGGCGTGCTGTACTGGCTTTCCCTGAAGATTATTTAA

Upstream 100 bases:

>100_bases
CACGAAGTACATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCCAAAGTCACTGATAATGCGCCGCGTTCATGTCC
TCAAAATGGCGTAACGTCCT

Downstream 100 bases:

>100_bases
GCCAAATGGGCCGGATAGCGCTGTCGCTAATCCGGCCTGTCAGGATGAAACGACACAGGCCTGACAGGCCGCTACAAGCG
GCGTCATCCGGAGGCTTATC

Product: low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNAMMTGTSVVDALNIPKVINIFGSLI
ISPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASSYEITRTRDAINNVETYFEQRPDLLKAVTGVDQLIPSPEPGATEPTEFHCHPANTINALNRAKG
MLANVESYDKLSVEQRSQLRRIMLCISDTTDKVVKLPGVSSDDQRLLKKLKTDMLSTIEYAPVWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTL
PAAIILSGVLYWLSLKII

Sequences:

>Translated_498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNAMMTGTSVVDALNIPKVINIFGSLI
ISPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASSYEITRTRDAINNVETYFEQRPDLLKAVTGVDQLIPSPEPGATEPTEFHCHPANTINALNRAKG
MLANVESYDKLSVEQRSQLRRIMLCISDTTDKVVKLPGVSSDDQRLLKKLKTDMLSTIEYAPVWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTL
PAAIILSGVLYWLSLKII
>Mature_498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNAMMTGTSVVDALNIPKVINIFGSLI
ISPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASSYEITRTRDAINNVETYFEQRPDLLKAVTGVDQLIPSPEPGATEPTEFHCHPANTINALNRAKG
MLANVESYDKLSVEQRSQLRRIMLCISDTTDKVVKLPGVSSDDQRLLKKLKTDMLSTIEYAPVWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTL
PAAIILSGVLYWLSLKII

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=92.3847695390782, Blast_Score=906, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=81.1623246492986, Blast_Score=783, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53490; Mature: 53490

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNAMMTGTSVVDALNIPKVINIFGSLIISPIVGLVFAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASSYEITRTRDAINNVETYFEQRPDLLKAVTGVDQLIPSPEPGATE
HHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC
PTEFHCHPANTINALNRAKGMLANVESYDKLSVEQRSQLRRIMLCISDTTDKVVKLPGVS
CCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
SDDQRLLKKLKTDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
PAAIILSGVLYWLSLKII
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVVMAAVF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNAMMTGTSVVDALNIPKVINIFGSLIISPIVGLVFAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVAFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASSYEITRTRDAINNVETYFEQRPDLLKAVTGVDQLIPSPEPGATE
HHHCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCC
PTEFHCHPANTINALNRAKGMLANVESYDKLSVEQRSQLRRIMLCISDTTDKVVKLPGVS
CCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
SDDQRLLKKLKTDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHH
PAAIILSGVLYWLSLKII
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]