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The map label for this gene is selB [H]

Identifier: 29144116

GI number: 29144116

Start: 3960899

End: 3962749

Strand: Direct

Name: selB [H]

Synonym: t3837

Alternate gene names: 29144116

Gene position: 3960899-3962749 (Clockwise)

Preceding gene: 29144115

Following gene: 29144118

Centisome position: 82.66

GC content: 56.89

Gene sequence:

>1851_bases
ATGATTATTGCAACTGCCGGACACGTAGACCACGGTAAAACGACGTTGCTCCAGGCGATTACCGGCATTAATGCCGATCG
CCTGCCGGAAGAGAAAAAACGCGGGATGACTATCGATCTGGGCTACGCCTATTGGCCGCAGCCGGATGGTCGTGTGCTGG
GCTTTATCGATGTGCCTGGGCATGAAAAATTCCTCTCTAACATGCTGGCGGGCGTAGGCGGTATCGATCACGCTTTGCTG
GTGGTCGCCTGTGATGACGGCGTTATGGCGCAAACGCGCGAGCATTTACAGATCCTACAGCTGACGGGCAATCTCCAGCT
CACCGTCGCGCTGACCAAAGCCGATCGCGTGGACGAGGCGCGTATCAGCGAAGTACGTGAAGAGGTGCTGGCGGCGCTCG
ACAACTATGGCTTCGCCGATACCGTTTTATTTGTCACCGCCGCCAATGAAGGACGCGGTATTGCAGAACTTCGCGCTCAT
TTACAGCAGCTTCCTGCACGTTTACACGCCGCGCAGCATCGCTTCCGTCTGGCTATCGACCGTGCGTTTACGGTGAAAGG
GGCGGGACTGGTGGTGACCGGAACGGCGTTGAGCGGAGAGGTAAACGTGGGCGATACGCTATGGTTGACCGGCGTTAATA
CGCCGATGCGCGTACGAAGTCTACACGCGCAAAATCAGCCTACCGACCATGCGTATGCCGGGCAACGTATTGCGCTCAAT
ATTGCGGGCGATGCGGAGAAGGAGCAGCTTAACCGCGGCGACTGGCTGCTTTCCGATGCGCCGGCGGGGGAGGCTTTTTC
CCGGGTGATCGTTTCTCTGGCGCTTCATGCGCCGCTTTCCCAATGGCAACCGCTACATATTCACCATGCCGCCAGCCATG
TGACCGGTCGCGTCTCTTTGCTGGAAGGCGCGCTGGCCGAGCTGATCTTTGATACGCCGTTGTGGCTGGCCGATAATGAC
AGACTGGTATTGCGTGATATTTCCGCACGCGCCACCCTTGCCGGCGCACGCGTGGTGACGCTTAACGCGCCGCGCCGCGG
TAAACGTAAGCCAGATTATTTGCACTGGCTTTCAACGCTTGCCGATGCGCAGGACGATAGCGCAGCGTTGGCCATTCATC
TGGAACGCGGGGCCGTCAACCTGCCGGATTTCGGTTGGGCGCGTCAGCTTAATCCTCTCGGTATGCGTCAGCTTATTGAG
CAACATGGTTTTATCCAGGCCGGAGACAATCTGTTAAGCGCGCCCGTCGCCGCCCGCTGGCAGCGTAAGATTCTTGATAC
GCTGGCGACCTACCACGAACAGCACCGCGATGAACCGGGGCCGGGACGCGAGCGTTTACGCCGCATGGCCCTGCCAATGG
AGGATGAAGCGCTGGTTCTGATGCTGATCGAGCGGATGCGCGACGAGGGCCTGATTCATAGCCATCACGGTTGGTTGCAT
CTGCCGGATCATAAAGCGGGATTTAGCGATGAGCAGCAGGCCGTCTGGCAAAAAGTCGAACCGCTATTTGGCGATGAGCC
CTGGTGGGTACGCGATCTGGCGAAAGAGACCGGAACGGAAGAACAATTGATGCGGCTGGTGCTGCGGCAGGCGGCACAGC
AAGGCATCATTACCGCGATCGTAAAAGATCGGTACTATCGTAACGATCGGATTGTCGCTTTCGCCAATATGATCCGCGAG
CTGGATCAAGAGAGAGGATCAACCTGTGCGGCGGATTTTCGCGACAGGCTGAATGTAGGGCGGAAACTGGCCATTCAGAT
TCTTGAATATTTTGACCGGATTGGATTTACACGACGTCGTGGGAATGATCATTTATTACGTGATGCGTTATTATTTCCGC
AAAAAGAATGA

Upstream 100 bases:

>100_bases
CGCTGCCTGTACCGGTCATTGGCCGAATTTATGATGGCCGTTTATGGCTGGATATGCGCTGTCTTGAAGACGAAAGCCGG
TTTATGGAGATGATGCTGAA

Downstream 100 bases:

>100_bases
AATGGTTAAATTAAATAACAAATGAAGCAATACAATTATTGCGTTGCTTTATAAAAAGCACATCAGCAAATGAACCTGAT
GTGCTTTTTATTTTTCTGGC

Product: selenocysteinyl-tRNA-specific translation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 616; Mature: 616

Protein sequence:

>616_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARISEVREEVLAALDNYGFADTVLFVTAANEGRGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNTPMRVRSLHAQNQPTDHAYAGQRIALN
IAGDAEKEQLNRGDWLLSDAPAGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGALAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLNAPRRGKRKPDYLHWLSTLADAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDEGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPQKE

Sequences:

>Translated_616_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARISEVREEVLAALDNYGFADTVLFVTAANEGRGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNTPMRVRSLHAQNQPTDHAYAGQRIALN
IAGDAEKEQLNRGDWLLSDAPAGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGALAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLNAPRRGKRKPDYLHWLSTLADAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDEGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPQKE
>Mature_616_residues
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPGHEKFLSNMLAGVGGIDHALL
VVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEARISEVREEVLAALDNYGFADTVLFVTAANEGRGIAELRAH
LQQLPARLHAAQHRFRLAIDRAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNTPMRVRSLHAQNQPTDHAYAGQRIALN
IAGDAEKEQLNRGDWLLSDAPAGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSLLEGALAELIFDTPLWLADND
RLVLRDISARATLAGARVVTLNAPRRGKRKPDYLHWLSTLADAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIE
QHGFIQAGDNLLSAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDEGLIHSHHGWLH
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAIVKDRYYRNDRIVAFANMIRE
LDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPQKE

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=280, Percent_Identity=32.5, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI54607086, Length=352, Percent_Identity=25.5681818181818, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI223555963, Length=374, Percent_Identity=25.9358288770053, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI5729864, Length=374, Percent_Identity=25.9358288770053, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI53729339, Length=250, Percent_Identity=29.6, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI53729337, Length=250, Percent_Identity=29.6, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI46094014, Length=381, Percent_Identity=23.6220472440945, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI194018522, Length=385, Percent_Identity=22.8571428571429, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI194097354, Length=381, Percent_Identity=23.0971128608924, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI194018520, Length=381, Percent_Identity=23.0971128608924, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI2367247, Length=615, Percent_Identity=85.0406504065041, Blast_Score=1066, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=367, Percent_Identity=27.7929155313352, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789737, Length=367, Percent_Identity=27.7929155313352, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1789108, Length=293, Percent_Identity=27.6450511945392, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1789559, Length=96, Percent_Identity=39.5833333333333, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=230, Percent_Identity=34.7826086956522, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17556456, Length=373, Percent_Identity=27.0777479892761, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17506081, Length=260, Percent_Identity=27.6923076923077, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI115532067, Length=326, Percent_Identity=25.7668711656442, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI115532065, Length=326, Percent_Identity=25.7668711656442, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17552884, Length=404, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17569207, Length=404, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=234, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=303, Percent_Identity=32.3432343234323, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325337, Length=409, Percent_Identity=23.960880195599, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319594, Length=409, Percent_Identity=23.960880195599, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324550, Length=128, Percent_Identity=32.03125, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320377, Length=281, Percent_Identity=24.1992882562278, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921738, Length=264, Percent_Identity=32.5757575757576, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI281363316, Length=279, Percent_Identity=31.1827956989247, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864358, Length=279, Percent_Identity=31.1827956989247, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922690, Length=260, Percent_Identity=32.3076923076923, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550900, Length=378, Percent_Identity=22.2222222222222, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45553807, Length=317, Percent_Identity=26.1829652996845, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45553816, Length=317, Percent_Identity=26.1829652996845, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24651721, Length=317, Percent_Identity=26.1829652996845, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864154, Length=317, Percent_Identity=26.1829652996845, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137380, Length=351, Percent_Identity=24.2165242165242, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652838, Length=406, Percent_Identity=23.6453201970443, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137572, Length=406, Percent_Identity=23.6453201970443, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=149, Percent_Identity=36.9127516778523, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 68752; Mature: 68752

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCEEEEEEEECCCCCHHH
RISEVREEVLAALDNYGFADTVLFVTAANEGRGIAELRAHLQQLPARLHAAQHRFRLAID
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
RAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNTPMRVRSLHAQNQPTDHAYAGQRIALN
HEEEEECCCEEEEECEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEE
IAGDAEKEQLNRGDWLLSDAPAGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSL
ECCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHCCCEEEEHHHHHHHHHHHH
LEGALAELIFDTPLWLADNDRLVLRDISARATLAGARVVTLNAPRRGKRKPDYLHWLSTL
HHHHHHHHHHCCCCEEECCCEEEEEECCCHHEECCCEEEEECCCCCCCCCCHHHHHHHHH
ADAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIEQHGFIQAGDNLLSAPVAARW
HCCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCEECCCCHHCCHHHHHH
QRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDEGLIHSHHGWLH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEE
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAI
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VKDRYYRNDRIVAFANMIRELDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRR
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
GNDHLLRDALLFPQKE
CCHHHHHHHHCCCCCC
>Mature Secondary Structure
MIIATAGHVDHGKTTLLQAITGINADRLPEEKKRGMTIDLGYAYWPQPDGRVLGFIDVPG
CEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECEEECCCCCCCEEEEEECCC
HEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLQILQLTGNLQLTVALTKADRVDEA
HHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHEEEEECCCEEEEEEEECCCCCHHH
RISEVREEVLAALDNYGFADTVLFVTAANEGRGIAELRAHLQQLPARLHAAQHRFRLAID
HHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEE
RAFTVKGAGLVVTGTALSGEVNVGDTLWLTGVNTPMRVRSLHAQNQPTDHAYAGQRIALN
HEEEEECCCEEEEECEECCCCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEE
IAGDAEKEQLNRGDWLLSDAPAGEAFSRVIVSLALHAPLSQWQPLHIHHAASHVTGRVSL
ECCCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHCCHHHCCCEEEEHHHHHHHHHHHH
LEGALAELIFDTPLWLADNDRLVLRDISARATLAGARVVTLNAPRRGKRKPDYLHWLSTL
HHHHHHHHHHCCCCEEECCCEEEEEECCCHHEECCCEEEEECCCCCCCCCCHHHHHHHHH
ADAQDDSAALAIHLERGAVNLPDFGWARQLNPLGMRQLIEQHGFIQAGDNLLSAPVAARW
HCCCCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCEECCCCHHCCHHHHHH
QRKILDTLATYHEQHRDEPGPGRERLRRMALPMEDEALVLMLIERMRDEGLIHSHHGWLH
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEECCCCEEE
LPDHKAGFSDEQQAVWQKVEPLFGDEPWWVRDLAKETGTEEQLMRLVLRQAAQQGIITAI
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHH
VKDRYYRNDRIVAFANMIRELDQERGSTCAADFRDRLNVGRKLAIQILEYFDRIGFTRRR
HHHHHHCCCCCHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
GNDHLLRDALLFPQKE
CCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2531290; 8041620; 9278503; 2140572 [H]