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The map label for this gene is nepI

Identifier: 29144031

GI number: 29144031

Start: 3855707

End: 3856900

Strand: Direct

Name: nepI

Synonym: t3742

Alternate gene names: 29144031

Gene position: 3855707-3856900 (Clockwise)

Preceding gene: 29144029

Following gene: 29144034

Centisome position: 80.46

GC content: 56.78

Gene sequence:

>1194_bases
ATGAATGAAAATATCGCAGAAAAATTCCGCGCTGATGGGGTTGCCAGGCCTAACTGGTCAGCCGTTTTTGCCGTGGCGTT
TTGTGTCGCCTGCCTGATTACCGTTGAGTTTTTGCCCGTTAGTCTGTTGACGCCGATGGCGCAGGATCTGGGGATCTCCG
AGGGCGTCGCCGGTCAGTCGGTCACCGTTACGGCGTTTGTCGCGATGTTTTCCAGCCTGTTCATTACCCAGATTATTCAG
GCGACCGACAGGCGTTATATCGTTATTCTGTTCGCCGTTTTGCTGACGGCTTCTTGTCTGATGGTCTCCTTTGCCAACAG
CTTTACGCTGCTATTGCTGGGCCGCGCCTGTCTTGGGTTGGCGCTGGGCGGATTCTGGGCGATGTCGGCGTCGCTGACCA
TGCGACTGGTTCCCGCGCGTACCGTGCCGAAAGCGCTGTCGGTGATTTTTGGCGCGGTCTCCATCGCGTTAGTGATCGCC
GCGCCGCTGGGCAGTTTTTTGGGCGGTATTATTGGCTGGCGTAATGTCTTTAACGCCGCTGCGGTGATGGGCGTACTGTG
CGTCATCTGGGTGGTGAAATCACTGCCGTCGCTGCCGGGCGAACCTTCTCACCAGAAACAGAATATGTTTAGCCTGTTGC
AACGCCCTGGCGTGATGGCCGGGATGATCGCCATCTTTATGTCTTTTGCCGGGCAGTTCGCTTTCTTTACCTATATTCGC
CCGGTCTATATGAATCTGGCGGGCTTTGACGTTGATGGTCTGACGCTGGTGCTGCTAAGTTTTGGTATTGCCAGCTTCGT
TGGCACTTCTTTCTCCTCTTACGTCCTGAAACGTTCGGTAAAACTGGCGCTGGCCGGTGCGCCGCTGCTACTGGCGCTGA
GCGCGCTGACGCTCATTGTGTGGGGAAGCGACAAAACCGTGGCGGCGGCAATAGCGATTATCTGGGGACTGGCGTTTGCG
TTGGTGCCGGTGGGATGGTCAACGTGGATCACTCGTTCTCTTGCCGATCAGGCGGAAAAAGCCGGTTCCATCCAGGTCGC
GGTGATTCAACTGGCAAATACCTGTGGCGCGGCGGTGGGCGGTTATGCGCTCGACAATTTCGGGCTGCTTTCGCCGCTGG
CGCTTTCCGGCGGTCTGATGCTGTTGACGGCGTTAGTCGTGGCGGCGAAAGTCCGTATTACGCCAATGAGTTGA

Upstream 100 bases:

>100_bases
TGCAGCGCAACAATGTCAGTTGCTAAAACGTTAATCAGATCACATTTTGTAATGTTACACTGCGTGGATTTTCCGCACTA
AAACGACGCAGGTAACCACA

Downstream 100 bases:

>100_bases
TATTGCTGCCATTTGTAGGCCGGATACGACGTTAGTCGTTATCCGGCAATAATAAACGCTTAATCAAAACCGTTATGCTC
TGCGGTGGCGGCAAACCATT

Product: ribonucleoside transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNENIAEKFRADGVARPNWSAVFAVAFCVACLITVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFSSLFITQIIQ
ATDRRYIVILFAVLLTASCLMVSFANSFTLLLLGRACLGLALGGFWAMSASLTMRLVPARTVPKALSVIFGAVSIALVIA
APLGSFLGGIIGWRNVFNAAAVMGVLCVIWVVKSLPSLPGEPSHQKQNMFSLLQRPGVMAGMIAIFMSFAGQFAFFTYIR
PVYMNLAGFDVDGLTLVLLSFGIASFVGTSFSSYVLKRSVKLALAGAPLLLALSALTLIVWGSDKTVAAAIAIIWGLAFA
LVPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAVGGYALDNFGLLSPLALSGGLMLLTALVVAAKVRITPMS

Sequences:

>Translated_397_residues
MNENIAEKFRADGVARPNWSAVFAVAFCVACLITVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFSSLFITQIIQ
ATDRRYIVILFAVLLTASCLMVSFANSFTLLLLGRACLGLALGGFWAMSASLTMRLVPARTVPKALSVIFGAVSIALVIA
APLGSFLGGIIGWRNVFNAAAVMGVLCVIWVVKSLPSLPGEPSHQKQNMFSLLQRPGVMAGMIAIFMSFAGQFAFFTYIR
PVYMNLAGFDVDGLTLVLLSFGIASFVGTSFSSYVLKRSVKLALAGAPLLLALSALTLIVWGSDKTVAAAIAIIWGLAFA
LVPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAVGGYALDNFGLLSPLALSGGLMLLTALVVAAKVRITPMS
>Mature_397_residues
MNENIAEKFRADGVARPNWSAVFAVAFCVACLITVEFLPVSLLTPMAQDLGISEGVAGQSVTVTAFVAMFSSLFITQIIQ
ATDRRYIVILFAVLLTASCLMVSFANSFTLLLLGRACLGLALGGFWAMSASLTMRLVPARTVPKALSVIFGAVSIALVIA
APLGSFLGGIIGWRNVFNAAAVMGVLCVIWVVKSLPSLPGEPSHQKQNMFSLLQRPGVMAGMIAIFMSFAGQFAFFTYIR
PVYMNLAGFDVDGLTLVLLSFGIASFVGTSFSSYVLKRSVKLALAGAPLLLALSALTLIVWGSDKTVAAAIAIIWGLAFA
LVPVGWSTWITRSLADQAEKAGSIQVAVIQLANTCGAAVGGYALDNFGLLSPLALSGGLMLLTALVVAAKVRITPMS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily

Homologues:

Organism=Escherichia coli, GI145693198, Length=393, Percent_Identity=87.2773536895674, Blast_Score=684, Evalue=0.0,
Organism=Escherichia coli, GI1787808, Length=234, Percent_Identity=30.3418803418803, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787947, Length=291, Percent_Identity=26.1168384879725, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786595, Length=330, Percent_Identity=25.1515151515152, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NEPI_SALCH (P0C566)

Other databases:

- EMBL:   AE017220
- ProteinModelPortal:   P0C566
- GenomeReviews:   AE017220_GR
- HOGENOM:   HBG732715
- HAMAP:   MF_01189
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196

Pfam domain/function: PF07690 MFS_1; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 41690; Mature: 41690

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x11f7a418)-; HASH(0x1204b760)-; HASH(0x105b6fd4)-; HASH(0xfd20d8c)-; HASH(0x40e1c634)-; HASH(0x119c5e7c)-; HASH(0x123109ec)-; HASH(0x1230943c)-; HASH(0x1237a2c8)-; HASH(0x1128caf4)-; HASH(0x40e1ce98)-; HASH(0x126133a8)-;

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHEEECCH
>Mature Secondary Structure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CHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHEEECCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA