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The map label for this gene is ilvB [H]

Identifier: 29144013

GI number: 29144013

Start: 3836613

End: 3838301

Strand: Direct

Name: ilvB [H]

Synonym: t3722

Alternate gene names: 29144013

Gene position: 3836613-3838301 (Clockwise)

Preceding gene: 29144012

Following gene: 29144014

Centisome position: 80.06

GC content: 56.07

Gene sequence:

>1689_bases
ATGGCAAGTTCGGGCACAACATCTACACCTCAGCGCTTTACGGGCGCGGAATTTATAGTTCATTTTCTGGAGCGCCAGGG
GATTCGTATCGTCACCGGCATTCCCGGCGGTTCGATCCTCCCCGTTTACGACGCGTTAAGCCAAAGCACGCAAATCCGTC
ATATTCTGGCGCGCCACGAACAAGGCGCGGGGTTTATTGCGCAGGGGATGGCGCGGACGGAGAGTAAACCTGCTGTTTGT
ATGGCCTGTAGCGGGCCAGGCGCGACCAATCTGGTCACGGCTATTGCCGATGCGCGCCTGGATTCCATTCCGTTAATCTG
CATTACCGGGCAGGTTCCGGCGTCCATGATCGGTACTGACGCGTTTCAGGAAGTGGATACCTACGGTATCTCTATCCCCA
TCACCAAGCACAACTATCTGGTCAGAAATATCAATGAGCTGCCTCAGGTGATGAGCGATGCTTTTCGTCTGGCGCAGTCG
GGGCGTCCTGGCCCGGTGTGGATAGACGTTCCTAAAGATGTGCAGACGGCGACCATTGAGCTGGATGCGTTGCCGTCGCC
GGCGGAAAAATCACCCCCGCCGGAGTTTAGCGCGGAGAGTATCCGCGAGGCCGCCGCAATGATTAACGCGGCGAAGCGTC
CGGTGCTGTATCTGGGCGGCGGCGTGATCAATGCGCCGACACGCGTGCGCGAACTGGCGGAAAAAGCGCAACTGCCGACG
ACAATGACCTTAATGGCGCTGGGAATGCTGCCTAAGGCGCATCCGCTGTCGTTAGGAATGCTGGGAATGCACGGCGCGCG
CAGTACCAATTTTATTCTGCAAGAGGCCGATTTACTGATTGTTCTGGGCGCGCGTTTTGATGACCGGGCGATTGGCAAAA
CGGAGCAGTTTTGTCCGAACGCCAAAATCATCCATGTGGATATCGATCGCGCCGAGTTAGGCAAAATTAAGCAGCCGCAT
GTGGCTATTCAGGCGGATGTCGATGATGTACTGGCACAGTTAATTCCCCATATCGAGGCACAGCCGCGGGAAGCATGGCA
TCAGTTGGTGGCGGACTTGCAGCAAGAATTCCCTTGCGGTATTCCCCAGGAAAATAACCCGCTTAGTCACTATGGGCTGA
TTAACGCGGTCGCAGCCTGTGTGGACGACAACGCGATTATTACCACTGACGTAGGTCAGCATCAGATGTGGACCGCGCAG
GCTTATCCGCTCAACCGTCCGCGTCAGTGGCTGACTTCCGGCGGGCTGGGCACCATGGGGTTTGGTCTGCCCGCGGCGAT
TGGCGCGGCGCTGGCGAATCCGGGCAACAAGGTGCTGTGTTTTTCCGGCGACGGTAGTTTGATGATGAATATTCAGGAGA
TGGCTACCGCCAGCGAAAACCAGTTGGATGTGAAAATCATCCTGATGAATAACGACGCGCTGGGACTGGTGCATCAGCAA
CAGAGTCTGTTCTATAAACAGGGCGTTTTTGCGGCAACCTATCCGGGAAGCATCAACTTTATGCAGATTGCCGCCGGGTT
TGGTCTGGACACCTGTGATTTGAATAACGAAGCCGATCCACAGGCGGCGTTGCAGGCTATCATTCGTCGTCCTGGACCGG
CGTTGATCCACGTGCGCATTGATGCAGAAGAAAAAGTGTATCCGATGGTGCCGCCGGGTGCGGCAAATACAGAGATGGTG
GGGGAATAA

Upstream 100 bases:

>100_bases
GAACACACACGATTCCAAAACCCCGCCGGCGCAAACCGGGCGGGGTTTTTTGTTTAAGACACCTTCCCGGAACGTCGGCT
CAGAATAAAAGGAATGGAGC

Downstream 100 bases:

>100_bases
GCCATGCAGAAACAATCACATGATAACGTTATTCTGGAGCTGACCGTTCGCAACCATCCTGGCGTGATGACCCACGTCTG
CGGGCTGTTCGCCCGACGCG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MASSGTTSTPQRFTGAEFIVHFLERQGIRIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTESKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRNINELPQVMSDAFRLAQS
GRPGPVWIDVPKDVQTATIELDALPSPAEKSPPPEFSAESIREAAAMINAAKRPVLYLGGGVINAPTRVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPHIEAQPREAWHQLVADLQQEFPCGIPQENNPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPGNKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNDALGLVHQQ
QSLFYKQGVFAATYPGSINFMQIAAGFGLDTCDLNNEADPQAALQAIIRRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE

Sequences:

>Translated_562_residues
MASSGTTSTPQRFTGAEFIVHFLERQGIRIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTESKPAVC
MACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRNINELPQVMSDAFRLAQS
GRPGPVWIDVPKDVQTATIELDALPSPAEKSPPPEFSAESIREAAAMINAAKRPVLYLGGGVINAPTRVRELAEKAQLPT
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQADVDDVLAQLIPHIEAQPREAWHQLVADLQQEFPCGIPQENNPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPGNKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNDALGLVHQQ
QSLFYKQGVFAATYPGSINFMQIAAGFGLDTCDLNNEADPQAALQAIIRRPGPALIHVRIDAEEKVYPMVPPGAANTEMV
GE
>Mature_561_residues
ASSGTTSTPQRFTGAEFIVHFLERQGIRIVTGIPGGSILPVYDALSQSTQIRHILARHEQGAGFIAQGMARTESKPAVCM
ACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRNINELPQVMSDAFRLAQSG
RPGPVWIDVPKDVQTATIELDALPSPAEKSPPPEFSAESIREAAAMINAAKRPVLYLGGGVINAPTRVRELAEKAQLPTT
MTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHV
AIQADVDDVLAQLIPHIEAQPREAWHQLVADLQQEFPCGIPQENNPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQA
YPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPGNKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNDALGLVHQQQ
SLFYKQGVFAATYPGSINFMQIAAGFGLDTCDLNNEADPQAALQAIIRRPGPALIHVRIDAEEKVYPMVPPGAANTEMVG
E

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=25.9717314487633, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI21361361, Length=559, Percent_Identity=25.9391771019678, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=91.8149466192171, Blast_Score=1053, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=38.86925795053, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=481, Percent_Identity=35.1351351351351, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787096, Length=548, Percent_Identity=27.9197080291971, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1788716, Length=481, Percent_Identity=28.2744282744283, Blast_Score=152, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=561, Percent_Identity=25.3119429590018, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=25.3119429590018, Blast_Score=153, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=530, Percent_Identity=25.8490566037736, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=39.8963730569948, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320816, Length=498, Percent_Identity=24.8995983935743, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=480, Percent_Identity=20.8333333333333, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321524, Length=485, Percent_Identity=20, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922626, Length=477, Percent_Identity=28.9308176100629, Blast_Score=177, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60225; Mature: 60094

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00187 TPP_ENZYMES ; PS01047 HMA_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSGTTSTPQRFTGAEFIVHFLERQGIRIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTESKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRNINELPQVMSDAFRLAQSGRPGPVWIDVPKDVQTATIE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEE
LDALPSPAEKSPPPEFSAESIREAAAMINAAKRPVLYLGGGVINAPTRVRELAEKAQLPT
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPHIEAQPREAWHQLVADLQQEFPCG
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
IPQENNPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPGNKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNDALGLVHQQ
CCHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGSINFMQIAAGFGLDTCDLNNEADPQAALQAIIRRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSGTTSTPQRFTGAEFIVHFLERQGIRIVTGIPGGSILPVYDALSQSTQIRHILARHE
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCEEHHHHHHCHHHHHHHHHHHCC
QGAGFIAQGMARTESKPAVCMACSGPGATNLVTAIADARLDSIPLICITGQVPASMIGTD
CCCCHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRNINELPQVMSDAFRLAQSGRPGPVWIDVPKDVQTATIE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEE
LDALPSPAEKSPPPEFSAESIREAAAMINAAKRPVLYLGGGVINAPTRVRELAEKAQLPT
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHCCCH
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQLIPHIEAQPREAWHQLVADLQQEFPCG
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
IPQENNPLSHYGLINAVAACVDDNAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPGNKVLCFSGDGSLMMNIQEMATASENQLDVKIILMNNDALGLVHQQ
CCHHHHHHHHHCCCCCEEEEEECCCCEEEEHHHHHCCCCCCEEEEEEEECCCCEEEEEHH
QSLFYKQGVFAATYPGSINFMQIAAGFGLDTCDLNNEADPQAALQAIIRRPGPALIHVRI
HHHHHHCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
DAEEKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]