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The map label for this gene is gidB [H]

Identifier: 29143941

GI number: 29143941

Start: 3752919

End: 3753542

Strand: Direct

Name: gidB [H]

Synonym: t3646

Alternate gene names: 29143941

Gene position: 3752919-3753542 (Clockwise)

Preceding gene: 29143940

Following gene: 29143942

Centisome position: 78.32

GC content: 51.28

Gene sequence:

>624_bases
GTGCTCAACAAACTCTCTCGTCTGCTGGCTGATGCCGGTATTTCTCTTACCGATCACCAGAAAACGCTGCTGGTCGCCTA
CGTTGATATGCTGCACAAATGGAACAAAGCATACAACCTGACGTCAGTGCGGGATCCCAACGAAATGTTGGTACGCCATA
TTCTCGACAGCATAGTCGTTGCGCCGTATCTGCAGGGACAGCGTTTTATCGATGTAGGAACAGGACCGGGGCTGCCGGGC
ATCCCGCTGGCTATTGTTCTCCCTGACGCGCATTTCACCTTACTCGACAGCCTGGGCAAGCGGGTACGTTTTCTGCGTCA
GGTCCAGCACGAACTGAAACTGGAAAACATCACGCCGGTTCAAAGCCGCGTTGAAGCCTATCCCTCCGAACCGCCGTTTG
ATGGCGTTATCAGCCGCGCTTTCGCATCGTTGAATGATATGGTGAGCTGGTGTCACCATCTGCCGGGAGAGAAAGGACGT
TTTTATGCCTTAAAAGGGCAATTGCCAGGGGATGAAATTGCCTCACTACCTGACAATTTTAGCGTCGAATCTGTTGAAAA
ATTACGCGTTCCGCAACTGGAAGGCGAGCGGCATCTGGTGATTATTAAGTCAAACAAAGTTTAA

Upstream 100 bases:

>100_bases
ACACTAATTTGGCGGGGGCGCTTCGCTTGCCCGCCCTACAAATTTGTAGGCCCGATAAGCGAAAGCGCATCGGGCATTTG
ATTATTAACAGGTAATGACC

Downstream 100 bases:

>100_bases
TTTTTATCAAAAAAAATAGAAAAAACGACGCGGCTTGTGTTAAAAATCGACAGGTATAACGCATTTCAGAGTTGTTACAT
TTAACTCTGGATTTACGGTT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLNKLSRLLADAGISLTDHQKTLLVAYVDMLHKWNKAYNLTSVRDPNEMLVRHILDSIVVAPYLQGQRFIDVGTGPGLPG
IPLAIVLPDAHFTLLDSLGKRVRFLRQVQHELKLENITPVQSRVEAYPSEPPFDGVISRAFASLNDMVSWCHHLPGEKGR
FYALKGQLPGDEIASLPDNFSVESVEKLRVPQLEGERHLVIIKSNKV

Sequences:

>Translated_207_residues
MLNKLSRLLADAGISLTDHQKTLLVAYVDMLHKWNKAYNLTSVRDPNEMLVRHILDSIVVAPYLQGQRFIDVGTGPGLPG
IPLAIVLPDAHFTLLDSLGKRVRFLRQVQHELKLENITPVQSRVEAYPSEPPFDGVISRAFASLNDMVSWCHHLPGEKGR
FYALKGQLPGDEIASLPDNFSVESVEKLRVPQLEGERHLVIIKSNKV
>Mature_207_residues
MLNKLSRLLADAGISLTDHQKTLLVAYVDMLHKWNKAYNLTSVRDPNEMLVRHILDSIVVAPYLQGQRFIDVGTGPGLPG
IPLAIVLPDAHFTLLDSLGKRVRFLRQVQHELKLENITPVQSRVEAYPSEPPFDGVISRAFASLNDMVSWCHHLPGEKGR
FYALKGQLPGDEIASLPDNFSVESVEKLRVPQLEGERHLVIIKSNKV

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA [H]

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family [H]

Homologues:

Organism=Escherichia coli, GI1790179, Length=207, Percent_Identity=86.4734299516908, Blast_Score=375, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003682 [H]

Pfam domain/function: PF02527 GidB [H]

EC number: =2.1.1.170 [H]

Molecular weight: Translated: 23175; Mature: 23175

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKLSRLLADAGISLTDHQKTLLVAYVDMLHKWNKAYNLTSVRDPNEMLVRHILDSIVV
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APYLQGQRFIDVGTGPGLPGIPLAIVLPDAHFTLLDSLGKRVRFLRQVQHELKLENITPV
HHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QSRVEAYPSEPPFDGVISRAFASLNDMVSWCHHLPGEKGRFYALKGQLPGDEIASLPDNF
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCC
SVESVEKLRVPQLEGERHLVIIKSNKV
CHHHHHHHCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MLNKLSRLLADAGISLTDHQKTLLVAYVDMLHKWNKAYNLTSVRDPNEMLVRHILDSIVV
CHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
APYLQGQRFIDVGTGPGLPGIPLAIVLPDAHFTLLDSLGKRVRFLRQVQHELKLENITPV
HHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QSRVEAYPSEPPFDGVISRAFASLNDMVSWCHHLPGEKGRFYALKGQLPGDEIASLPDNF
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCC
SVESVEKLRVPQLEGERHLVIIKSNKV
CHHHHHHHCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA