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The map label for this gene is aes [H]

Identifier: 29143890

GI number: 29143890

Start: 3689573

End: 3690502

Strand: Direct

Name: aes [H]

Synonym: t3589

Alternate gene names: 29143890

Gene position: 3689573-3690502 (Clockwise)

Preceding gene: 29143887

Following gene: 29143891

Centisome position: 77.0

GC content: 55.48

Gene sequence:

>930_bases
ATGGCACTGGAAAGCGGGATTGCGCAGTTAGTGGAGGAATTTATCGCGGCGGGACGCCCCTCTTCTCGCGAACAGAATAT
TGATGACCGGAGGGCGGGCTACATCGCTAGCACGACATTGGCAGGAGAAACAGAAACCCGCGTCCAGGTTGAAGATATTG
AACTCGATGCCATGACGTTTCGCGTGGTTTCGCCGCTCAATGCCACAGGGAAATTACCCTGCATCATTTATTATCATGGT
GGCTGCTTTGTCAGCGGCGGTTTTGCCACTCACGATAACCAGTTAAGGCAGTTAGCTTTTTACAGCCGTTGTCGCGTGAT
TGCGGCACAGTACCGACTTGCACCAGAGCACACCTTCCCGGCCGCACATAACGATGCCGAAACCGGAGCCAATACTATCT
GGAAATATGCCCAGAAACTGGGGATAGATCGCGAGAACATTACGCTTGCTGGTGACAGCGCGGGTGGGCATCTGGCGCTG
GTCACCGCGCTACGCCTGAAAGCCGCCCGTCAATGGCAACCTGCACAGCTGATTCTGATCTACCCCATGCTGGACGCAAC
CGCCAGGTTTGCGAGCTACACCTGTAATGGGCTGGATTACATTATCACGCGTGACACGCTGCTTTCAGGCTACGAGATGT
ATATGCCGCGAACCGATCCACTGCATCCTGAAGCCAGTCCGCTCTGGCGTGAGGACTTCGCCGGTCTGCCATCCACGCAC
ATCATTACTGCCGAATTCGACCCACTGCGCGATGAGGGCGAGGCCCTGTATCAACGGTTCCAGGAACAGGGTGTGGAATG
CACTTGCCAGCGCTATCTCGGCGTTATCCACGGGTTTTTCCAATTGGGCGGAGTGAGCAAGACGGCGCGGGACGCAATAC
GCGACGTGGCCTGGCGTGTTGTAAGTCCATCAACAAGCAAAATGACCTAA

Upstream 100 bases:

>100_bases
AGCCGCTTTCCAGAATCGTACGCAGCCCAATTTTCCCATTTTCATTCGAACCAAATCGAGTTATAAAACTGTATATCCAT
ACAGATAAAGGCAATGAAAA

Downstream 100 bases:

>100_bases
AATTCAATTTTGTTTTCAACTTGAAAACTGGATAACGTTGCGCTAAATTGTTTTCAGGTTGAAAACTTTGTAATGGAACT
ACATTGTGCATGTTATCAGC

Product: lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MALESGIAQLVEEFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHG
GCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPEHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLAL
VTALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSKTARDAIRDVAWRVVSPSTSKMT

Sequences:

>Translated_309_residues
MALESGIAQLVEEFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHG
GCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPEHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLAL
VTALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSKTARDAIRDVAWRVVSPSTSKMT
>Mature_308_residues
ALESGIAQLVEEFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTFRVVSPLNATGKLPCIIYYHGG
CFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPEHTFPAAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALV
TALRLKAARQWQPAQLILIYPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTHI
ITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSKTARDAIRDVAWRVVSPSTSKMT

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.4436090225564, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786682, Length=226, Percent_Identity=32.7433628318584, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71996133, Length=128, Percent_Identity=38.28125, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17540028, Length=121, Percent_Identity=34.7107438016529, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=114, Percent_Identity=35.9649122807018, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17567059, Length=146, Percent_Identity=30.1369863013699, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34301; Mature: 34170

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALESGIAQLVEEFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTF
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEECCCCCCEEEEEEEEEEEEEE
RVVSPLNATGKLPCIIYYHGGCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPEHTFP
EEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALVTALRLKAARQWQPAQLILI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
EECHHHHHHHHHHCCCCEEEEEEHHHHHCCHHEECCCCCCCCCCCCCCHHHHHCCCCCCE
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSKTARDAIRDVAWRV
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHE
VSPSTSKMT
ECCCCCCCC
>Mature Secondary Structure 
ALESGIAQLVEEFIAAGRPSSREQNIDDRRAGYIASTTLAGETETRVQVEDIELDAMTF
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCEEEEEEECCCCCCEEEEEEEEEEEEEE
RVVSPLNATGKLPCIIYYHGGCFVSGGFATHDNQLRQLAFYSRCRVIAAQYRLAPEHTFP
EEECCCCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AAHNDAETGANTIWKYAQKLGIDRENITLAGDSAGGHLALVTALRLKAARQWQPAQLILI
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEE
YPMLDATARFASYTCNGLDYIITRDTLLSGYEMYMPRTDPLHPEASPLWREDFAGLPSTH
EECHHHHHHHHHHCCCCEEEEEEHHHHHCCHHEECCCCCCCCCCCCCCHHHHHCCCCCCE
IITAEFDPLRDEGEALYQRFQEQGVECTCQRYLGVIHGFFQLGGVSKTARDAIRDVAWRV
EEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHE
VSPSTSKMT
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA