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The map label for this gene is nfi [H]

Identifier: 29143777

GI number: 29143777

Start: 3552303

End: 3552974

Strand: Reverse

Name: nfi [H]

Synonym: t3463

Alternate gene names: 29143777

Gene position: 3552974-3552303 (Counterclockwise)

Preceding gene: 29143778

Following gene: 29143776

Centisome position: 74.14

GC content: 58.63

Gene sequence:

>672_bases
ATGGATCTCGCGTCGCTACGCGCTCAACAGATAGAACTTGCTTCATCAGTTTGCCGCGAAGATCGCCTGGACAAAGATCC
GCCAGCGTTTATCGGCGGGGCGGACGTCGGTTTTGAGCAGGGCGGCGAGGTGACGCGGGCGGCGATGGTATTGCTGAAAT
ATCCGTCGCTTGAGCTAGTAGAGTACAAGGTGGCGCGTATTGCCACCACCATGCCGTATATCCCCGGCTTTCTCTCTTTT
CGTGAATATCCTGCGCTGCTGGCAGCGTGGGAACAGCTCTCGCAAAAGCCCGATTTGCTGTTTGTGGACGGTCACGGTAT
TTCGCATCCGCGTCGGCTTGGCGTCGCCAGTCATTTTGGACTGTTGGTGGACGTGCCGACCATCGGCGTGGCGAAAAAAC
GGCTATGCGGTAAATTTGAACCACTTTCCGCCGAACCTGGCGCACTTAGCCCCTTAATGGATAAAGGCGAGCAACTGGCG
TGGGTATGGCGCAGCAAGGCGCGCTGTAATCCGCTGTTTATCGCGACAGGGCACCGGGTCAGTACTGACAGCGCGCTGGC
GTGGGTACAGCGCTGTATGAAAGGGTATCGCCTGCCGGAGCCGACGCGCTGGGCGGATGCCGTCGCTTCCGGGCGTCCGG
CGTTTGTTCGTTGGCAAGAAATTCAGCGCTGA

Upstream 100 bases:

>100_bases
TGGACATGGCATCCATCAGGATGTGCCGCCAGAACATGCTGGCGCATTTGTGGAGGCAGTGCACCGACTTTCTGCGCAGT
ATCACAACTAAGGAGTTAGT

Downstream 100 bases:

>100_bases
TTCAGGTAAACTGCCGCTAATTTCCGATTCGAGATTCCATCATGTTACAAAACCCGATTCATTTGCGGCTGGAGCGGCTG
GAAAGTTGGCAGCACGTTAC

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MDLASLRAQQIELASSVCREDRLDKDPPAFIGGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEQLSQKPDLLFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSAEPGALSPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSTDSALAWVQRCMKGYRLPEPTRWADAVASGRPAFVRWQEIQR

Sequences:

>Translated_223_residues
MDLASLRAQQIELASSVCREDRLDKDPPAFIGGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEQLSQKPDLLFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSAEPGALSPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSTDSALAWVQRCMKGYRLPEPTRWADAVASGRPAFVRWQEIQR
>Mature_223_residues
MDLASLRAQQIELASSVCREDRLDKDPPAFIGGADVGFEQGGEVTRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSF
REYPALLAAWEQLSQKPDLLFVDGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSAEPGALSPLMDKGEQLA
WVWRSKARCNPLFIATGHRVSTDSALAWVQRCMKGYRLPEPTRWADAVASGRPAFVRWQEIQR

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=202, Percent_Identity=39.1089108910891, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI257467548, Length=197, Percent_Identity=31.4720812182741, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI257467550, Length=197, Percent_Identity=31.4720812182741, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI87082357, Length=222, Percent_Identity=93.6936936936937, Blast_Score=425, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 24746; Mature: 24746

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLASLRAQQIELASSVCREDRLDKDPPAFIGGADVGFEQGGEVTRAAMVLLKYPSLELV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
EYKVARIATTMPYIPGFLSFREYPALLAAWEQLSQKPDLLFVDGHGISHPRRLGVASHFG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCHHHHCC
LLVDVPTIGVAKKRLCGKFEPLSAEPGALSPLMDKGEQLAWVWRSKARCNPLFIATGHRV
EEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEECCCCC
STDSALAWVQRCMKGYRLPEPTRWADAVASGRPAFVRWQEIQR
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEHHHHCC
>Mature Secondary Structure
MDLASLRAQQIELASSVCREDRLDKDPPAFIGGADVGFEQGGEVTRAAMVLLKYPSLELV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
EYKVARIATTMPYIPGFLSFREYPALLAAWEQLSQKPDLLFVDGHGISHPRRLGVASHFG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHCHHHHCC
LLVDVPTIGVAKKRLCGKFEPLSAEPGALSPLMDKGEQLAWVWRSKARCNPLFIATGHRV
EEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHCCCCEEEEECCCCCCCEEEEECCCCC
STDSALAWVQRCMKGYRLPEPTRWADAVASGRPAFVRWQEIQR
CCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA