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The map label for this gene is nucD [H]

Identifier: 29143745

GI number: 29143745

Start: 3519096

End: 3519569

Strand: Direct

Name: nucD [H]

Synonym: t3424

Alternate gene names: 29143745

Gene position: 3519096-3519569 (Clockwise)

Preceding gene: 29143744

Following gene: 29143746

Centisome position: 73.44

GC content: 58.44

Gene sequence:

>474_bases
GTGCTGGCTATTGCTGCCACGCTGCCGGGTTTTCAGCAGCTTCACACCTCCGTGGAGGGGCTGAAACTGATTGCCGATTA
CGAAGGTTGTCGTCTGCAGCCGTATCAGTGCAGCGCGGGTGTCTGGACCGACGGCATTGGTAATACGTCAGGCGTCATTC
CCGGCAAAACCATTACGGAGCGACAGGCAGCAGAAGGGCTGATCTCCAACGTGCTGCGTGTGGAGCGGGCGCTGGAAAGG
TGTGTGAAGCAACAGCCGCCGCAGAAGGTGTATGACGCTGCGGTGTCGTTTGCCTTCAACGTGGGAACGGGCAATGCCTG
CAGTTCCACACTGGTGAAATTACTCAATCAGCGGCGCTGGGCGGATGCGTGCCGACAGTTGCCGCGCTGGGTTTATGTGA
AAGGTGTTTTTAATCAGGGGCTGGATAACCGCCGTGCGCGGGAGATGGCCTGGTGCTTACAGGGAGCAAACTGA

Upstream 100 bases:

>100_bases
TGGTACTACAAACACAAAGCCTACCAGCTTCTGCGCGACGGGCAGATCTCGCGGGAGGACTATGAATCCATCAATCGTTA
AACGCTGCCTTGTCGGGGCC

Downstream 100 bases:

>100_bases
AATGAAAAAGAAATTAATCAGCGGACTGTTTCTGATGTTATGGATGGCGCTGTTAATCGCAGCAATGGTGTATCCGCAGG
GGATTTTTCCGGTACTGGCA

Product: lysozyme

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MLAIAATLPGFQQLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALER
CVKQQPPQKVYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAREMAWCLQGAN

Sequences:

>Translated_157_residues
MLAIAATLPGFQQLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALER
CVKQQPPQKVYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAREMAWCLQGAN
>Mature_157_residues
MLAIAATLPGFQQLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALER
CVKQQPPQKVYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAREMAWCLQGAN

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=129, Percent_Identity=37.2093023255814, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 17269; Mature: 17269

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAIAATLPGFQQLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVIPGKTITE
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQAAEGLISNVLRVERALERCVKQQPPQKVYDAAVSFAFNVGTGNACSSTLVKLLNQRRW
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ADACRQLPRWVYVKGVFNQGLDNRRAREMAWCLQGAN
HHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MLAIAATLPGFQQLHTSVEGLKLIADYEGCRLQPYQCSAGVWTDGIGNTSGVIPGKTITE
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
RQAAEGLISNVLRVERALERCVKQQPPQKVYDAAVSFAFNVGTGNACSSTLVKLLNQRRW
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
ADACRQLPRWVYVKGVFNQGLDNRRAREMAWCLQGAN
HHHHHHCCHHEEEHHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]